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Mouse anti gad67

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Mouse anti-GAD67 is a primary antibody that recognizes the glutamic acid decarboxylase 67 (GAD67) protein, a key enzyme involved in the synthesis of the neurotransmitter gamma-aminobutyric acid (GABA) in the central nervous system.

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Lab products found in correlation

Las af, by Leica (2 mentions) Mouse anti neun, by Merck Group (2 mentions) Anti rabbit cy3, by Jackson ImmunoResearch (2 mentions) Anti mouse alexa 488, by Jackson ImmunoResearch (2 mentions) Anti mouse alexa 546, by Jackson ImmunoResearch (2 mentions) Draq5, by Enzo Life Sciences (2 mentions) Anti h3k9me3, by Active Motif (2 mentions)

Spelling variants of this product

GAD67 (13 citations) Anti-GAD67 (7 citations) Mouse anti (2 citations)

2 protocols using mouse anti gad67

1

Quantification of H3K9me3 in Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostainings were performed as described in SM on 10 μm coronal brain sections using rabbit anti-H3K9me3 (1:500, Active Motif), mouse anti-GAD67 (1:500, Abcam), and mouse anti-NeuN (1:500, Millipore) antibodies followed by anti-rabbit CY3 (1:500, Jackson laboratories) or anti-mouse Alexa-488 (1:800, Jackson Laboratories) or anti-mouse Alexa-546 (1:500, Jackson Laboratories) antibodies. Nuclei were counterstained with Draq5 (1:500, Enzo Life Science). Images were taken at the confocal (DMRE; SP5 Leica) and quantified using LAS AF (Leica) in 3 regions of interest (ROI) of 145x145 μm/image (in Fig. 2f n=16-18 images/mouse 3mice/genotype; in Fig. 3f n=14 images/mouse 3mice/genotype). Data represent ratios of pixels fluorescence/Draq5 per ROIs.
Brami-Cherrier K., Anzalone A., Ramos M., Forne I., Macciardi F., Imhof A, & Borrelli E. (2014). Epigenetic Reprogramming of Cortical Neurons through Alteration of Dopaminergic Circuits. Molecular psychiatry, 19(11), 1193-1200.
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2

Quantification of H3K9me3 in Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostainings were performed as described in SM on 10 μm coronal brain sections using rabbit anti-H3K9me3 (1:500, Active Motif), mouse anti-GAD67 (1:500, Abcam), and mouse anti-NeuN (1:500, Millipore) antibodies followed by anti-rabbit CY3 (1:500, Jackson laboratories) or anti-mouse Alexa-488 (1:800, Jackson Laboratories) or anti-mouse Alexa-546 (1:500, Jackson Laboratories) antibodies. Nuclei were counterstained with Draq5 (1:500, Enzo Life Science). Images were taken at the confocal (DMRE; SP5 Leica) and quantified using LAS AF (Leica) in 3 regions of interest (ROI) of 145x145 μm/image (in Fig. 2f n=16-18 images/mouse 3mice/genotype; in Fig. 3f n=14 images/mouse 3mice/genotype). Data represent ratios of pixels fluorescence/Draq5 per ROIs.
Brami-Cherrier K., Anzalone A., Ramos M., Forne I., Macciardi F., Imhof A, & Borrelli E. (2014). Epigenetic Reprogramming of Cortical Neurons through Alteration of Dopaminergic Circuits. Molecular psychiatry, 19(11), 1193-1200.
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