Intracellular levels of ATP were determined using the CellTitre-Glo 2.0 Cell Viability assay (Promega). Cells were treated with the CuSO4 inducer for 36 hours before measuring ATP. 10,000 cells per treatment condition were used to assess ATP levels, following the manufacturer’s protocol. Luminescence was read using the SpectraMax i3x plate reader (Molecular Devices). The experiment was performed with six technical and three biological replicates for each cell type tested. All values were normalized to cell number.
Celltitre glo 2.0 cell viability assay
Manufactured by Promega
CellTitre-Glo 2.0 Cell Viability Assay is a luminescent cell-based assay that quantifies the amount of ATP present, which indicates the number of metabolically active cells. The assay is designed to measure the viability of cells in culture.
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3 protocols using celltitre glo 2.0 cell viability assay
1
Intracellular ATP Quantification in Cells
2
Cell Viability Assay Using CellTiter-Glo 2.0
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Cell viability after siRNA transfection or 24 h compound incubation was measured with the CellTitre Glo 2.0 cell viability assay (Promega, cat. G9241), scaling the manufacturer’s protocol to 384-well plates and measuring luminescence with an Envision plate reader (Perkin Elmer). Results are shown in Figure S6 of the Supporting Information.
3
Intracellular ATP Quantification Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Intracellular levels of ATP were determined using the CellTitre-Glo 2.0 Cell Viability assay (Promega). Cells were treated with the CuSO4 inducer for 36 hours before measuring ATP. 10,000 cells per treatment condition were used to assess ATP levels, following the manufacturer's protocol. Luminescence was read using the SpectraMax i3x plate reader (Molecular Devices). The experiment was performed with six technical and three biological replicates for each cell type tested. All values were normalized to cell number.
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