The measurement of proton nuclear magnetic resonance (1H NMR) of the polymers were conducted on a JEOL ECA-500 spectrometer operating at 500 MHz. Fourier transform infrared (FT-IR) spectra were obtained using a JASCO FT-IR-4600 spectrometer by diffusing the samples in KBr pellets. GPC analyses of c-S-DTB and c-S were conducted by TOSOH HLC-8220 apparatus, using DMF solution containing 10.0 mM of LiBr and 10.0 mM of H3PO4 as eluent. The polymersome and its silica hybrids were observed using a HITACHI SU8010 scanning electron microscope (SEM) at 5 kV after samples were sputtered with Pt particles. The TEM observation was performed on a JEOL JEM-2010 instrument with an acceleration voltage of 200 kV. The samples for the TEM were prepared by dropping a suspension sample self-assembled onto a commercial copper micro grid coated with a polymer film. The samples on grid were thoroughly dried in vacuum at room temperature for 24 h prior to observation. The content of silica hybrids (c-iEP@SiO2) were investigated using a Seiko Instruments EXSTER 6000 thermogravimetric analysis (TGA) at 30–900 °C and a heating rate of 10 °C min−1 under air atmosphere.
Hlc 8220
Manufactured by Tosoh
Sourced in Japan
The HLC-8220 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a compact, modular design and provides consistent, reliable performance. The HLC-8220 is capable of handling a wide range of sample types and separation techniques.
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Lab products found in correlation
5 protocols using hlc 8220
1
Characterization of Polymersome Silica Hybrids
2
Characterization of Poly(ester amide)s
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1H NMR spectra were recorded on a Varian System 500 spectrometer (Agilent, Santa Clara, CA, USA) (500 MHz for 1H nucleus). Chemical shifts of 1H NMR spectra were calibrated by using residual proton in CDCl3 (δ = 7.26 ppm) and in DMSO-d6 (δ = 2.50 ppm). Molecular weights of poly(ester amide)s were determined using a GPC system (HLC-8220, Tosoh, Tokyo, Japan) at AIST. Poly(methyl methacrylate)s were used as standard substances. A column (TSK gel Super HM-N, Tosoh, Tokyo, Japan) was used with HFIP as an eluent (0.2 mL/min, 40 °C). The Tm and Tg of the poly(ester amide)s were measured by DSC (DSC-6220, Seiko Instruments, Tokyo, Japan). TG was performed using TG/DTA-6300 (Seiko Instruments, Tokyo, Japan) in a nitrogen stream.
3
Characterization of Water-Dispersed Biodegradable PU
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The viscosity, average particle size, solid content, and molecular weight of water-dispersed biodegradable PU samples were determined. Namely, the viscosity was measured by a single cylindrical rotational viscometer, Bismetron (Shibaura System Co., Ltd., Tokyo, Japan), under conditions of 50% relative humidity at 25 °C. The average particle size was measured by a light scattering photometer, ELSZ-2000 (Otsuka Electronics Co., Ltd., Osaka, Japan) under conditions of 50% relative humidity at 25 °C, and the data were analyzed by cumulant method. The concentration of the aqueous solution was 1.7 wt%. The solid content was obtained from the difference in weight before and after drying. The molecular weight was determined by gel permeation chromatography, HLC-8220 (Tosoh Co., Ltd., Tokyo, Japan) using column TSK gel (Tosoh Co., Ltd., Tokyo Japan). The interpretation of the results was based on the conventional calibration of columns with polystyrene standards.
4
Bipyridine-based Polymer Synthesis
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Bipyridine precursor was synthesized according to the reported procedure [29 (link)]. A mixture of bipyridine (1.5 g, 8.0 mmol) and tetraethylene glycol di(p-toluenesulfonate) (4.0 g, 8.0 mmol) was polymerized by stirring at 60 °C for 25 min, 110 °C for 70 min, and 120 °C for 120 min. The product was extracted by chloroform, washed by water, and reprecipitated to acetone several times. Finally, counter ion was exchanged by pouring the polymer solution into an excess amount of LiTFSI aqueous solution [3 (link)] to obtain 2 as a brown viscous solid (92% yield).
1H NMR (500 MHz; DMSO-d6): δ 9.24 (s, 2H, Ph), 9.07 (d, 2H, Ph), 8.15 (d, 2H, Ph), 4.84 (br, 4H, N+–CH2–), 4.00 (t, 4H, –O–CH2–), 3.62 (t, 4H, –O–CH2–), 3.52 (t, 4H, –O–CH2–), 2.21 (s, 6H, CH3). Mn = 3900. Mw/Mn = 1.3. Molecular weight was measured by size-exclusion chromatography (SEC, TOSOH HLC-8220) using methanol/acetic acid = 7/3 (vol/vol) containing 0.5 M sodium acetate as eluent. Polyethylene oxide was used as standard.
1H NMR (500 MHz; DMSO-d6): δ 9.24 (s, 2H, Ph), 9.07 (d, 2H, Ph), 8.15 (d, 2H, Ph), 4.84 (br, 4H, N+–CH2–), 4.00 (t, 4H, –O–CH2–), 3.62 (t, 4H, –O–CH2–), 3.52 (t, 4H, –O–CH2–), 2.21 (s, 6H, CH3). Mn = 3900. Mw/Mn = 1.3. Molecular weight was measured by size-exclusion chromatography (SEC, TOSOH HLC-8220) using methanol/acetic acid = 7/3 (vol/vol) containing 0.5 M sodium acetate as eluent. Polyethylene oxide was used as standard.
5
Gel Permeation Chromatography of PR
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Gel permeation chromatography (GPC) measurements to measure the molecular weight of the obtained PR were performed on HLC-8220 (TOSOH) with 10 mmol L−1 LiBr/DMSO solution as eluent. The PR–hpCD and PR–CD solutions were filtered using a hydrophobic filter (pore size: 0.2 μm). The molecular weight of the PR was estimated using the elution time of PEG standards.
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