Caco-2 cells (DS Pharma Biomedical Co., Ltd., Tokyo, Japan) were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin G potassium, 100 μg/mL streptomycin, and 1% nonessential amino acids [50 (link)]. Cells were plated on a 24-well Transwell plate or 6-well Transwell plate at a density of 8 × 104 cells/cm2 and incubated in a CO2 incubator at 37 °C for 21 days. Experiments were performed using cells that had previously been passaged 50 to 55 times. The integrity of the monolayer was measured by determining the TEER with an Epithelial Voltohmmeter (EVOM2, World Precision Instruments, Sarasota, FL, USA) [51 (link)].
Caco 2 cells
Manufactured by Sumitomo Pharma
Sourced in Japan
Caco-2 cells are a human epithelial colorectal adenocarcinoma cell line commonly used in pharmaceutical research. They are a well-established in vitro model for studying intestinal drug absorption and permeability.
Automatically generated - may contain errors
Lab products found in correlation
Epithelial volt ohm meter evom2, by World Precision Instruments (1 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions)
Mem non essential amino acids, by Fujifilm (1 mentions)
L alanyl l glutamine, by Fujifilm (1 mentions)
293ft cell, by Thermo Fisher Scientific (1 mentions)
Recombinant human insulin, by Fujifilm (1 mentions)
Capryol pgmc, by Gattefosse (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
2 4 2 hydroxyethyl 1 piperazinyl ethanesulfonic acid hepes, by Dojindo Laboratories (1 mentions)
Ldh cytotoxicity test, by Fujifilm (1 mentions)
Triton x 100, by Nacalai Tesque (1 mentions)
Glucose c2 test, by Fujifilm (1 mentions)
Antibiotic antimycotic mixed stock solution, by Nacalai Tesque (1 mentions)
Lauroglycol 90, by Gattefosse (1 mentions)
Amphotericin b, by Nacalai Tesque (1 mentions)
Maisine 35 1, by Gattefosse (1 mentions)
Fetal bovine serum (fbs), by Equitech-Bio (1 mentions)
Lauroglycol fcc, by Gattefosse (1 mentions)
Labrafil m 2125 cs, by Gattefosse (1 mentions)
4 protocols using caco 2 cells
1
Caco-2 cell monolayer transepithelial transport
2
Culturing Human Colon and Kidney Cells
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Cell Culture Human colon cancer Caco-2 cells (passages 35-70) were obtained from DS Pharma Biomedical (Osaka, Japan) and grown with Dulbecco's modified Eagle's medium (DMEM) (Wako Pure Chemical Industries, Ltd., Osaka, Japan) supplemented with heat-inactivated 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 µg/mL streptomycin in a humidified atmosphere of 5% CO 2 /air at 37°C.
Human embryotic kidney 293FT cells (passages 6-10) (Thermo Fisher Scientific, Waltham, MA, U.S.A.) were grown with DMEM supplemented with 10% FBS, 100 U/mL penicillin, 100 µg/mL streptomycin, 500 µg/mL G418 (Wako), 2 mM L-alanyl-L-glutamine (Wako), and 1% (v/v) MEM non-essential amino acids (Wako) in a humidified atmosphere of 5% CO 2 /air at 37°C.
Human embryotic kidney 293FT cells (passages 6-10) (Thermo Fisher Scientific, Waltham, MA, U.S.A.) were grown with DMEM supplemented with 10% FBS, 100 U/mL penicillin, 100 µg/mL streptomycin, 500 µg/mL G418 (Wako), 2 mM L-alanyl-L-glutamine (Wako), and 1% (v/v) MEM non-essential amino acids (Wako) in a humidified atmosphere of 5% CO 2 /air at 37°C.
3
Caco-2 Cell Culture and Transport Assay
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Caco-2 cells from Dainippon Sumitomo Pharma (Osaka, Japan) were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum, 1% antibiotic-antimycotic, 1% nonessential amino acids, and 2 mM l -glutamine [31 (link)]. Cells were maintained at 37 °C in 5% CO2/95% air and passaged upon reaching approximately 80% confluence using 0.25% trypsin/1 mM EDTA solution. For transport experiments, Caco-2 cells were grown on a polyethylene terephthalate insert (0.4 μm pore size, 12 mm diameter) at 1.0 × 105 cells/well, and cultured for 14–21 days with the replacement of the medium once every 2 days. Caco-2 cells used in this study were within the passage range of 55 through 70.
4
Caco-2 Cell Lipid Solubility Assay
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Male Wistar rats (220–270 g, 8 weeks of age) were obtained from SLC, Inc. (Hamamatsu, Shizuoka, Japan). The Glucose C2 Test, LDH Cytotoxicity Test and recombinant human insulin were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Labrasol®, Capryol 90, Capryol PGMC, Lauroglycol 90, Lauroglycol FCC, Labrafil M 2125 CS, and Maisine 35-1 were obtained from Gattefossé (Saint-Priest, France). Hydrogenated castor oil-60 (HCO-60) was obtained from Nikko Chemical Co., Ltd. (Osaka, Japan). Triton X-100, high glucose Dulbecco’s modified Eagle’s medium (DMEM), MEM non-essential amino acid solution (MEM-NEAA), and antibiotic–antimycotic mixed stock solution (10,000 IU/mL penicillin, 10 mg/mL streptomycin, and 25 μg/mL amphotericin B) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Fetal bovine serum (FBS) was obtained from Equitech-Bio, Inc. (Kerrville, TX, USA). Hank’s balanced salt (HBS) was obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). 2-[4-(2-Hydroxyethyl)-1-piperazinyl] ethanesulfonic acid (HEPES) was prepared by Dojindo Laboratories (Kumamoto, Japan). Caco-2 cells were bought from Dainippon Sumitomo Pharma Co., Ltd. (Osaka, Japan).
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