By using tetramethyl silane as the internal standard and DMSO‐d6 as deuterium solvent, the 1H NMR and 13C nuclear magnetic resonance (NMR) spectra were collected through a high‐resolution 400 MHz NMR spectrometer (Burker, Germany). The mass spectra were measured with a Q Exactive‐type four‐stage rod Orbitrap high‐resolution mass spectrometer (HRMS, UHPLC‐Q‐Orbitrap‐HRMS, Thermo Fisher Scientific, USA) and an Agilent Technologies 6530 TOF LC/MS (Agilent, Japan). The fluorescence spectra were collected on an Edinburgh FLS1000 fluorescence spectrophotometer (Edinburgh Instruments, UK). The colorimetric and fluorescent images were obtained by the iPhone 13 mini (Apple Inc., USA) or captured by an industrial camera (Vision Datum Mars 5000S‐20gc). Field‐emission scanning electron microscopy (FE‐SEM, JEOL JSM‐7610 F Plus, Japan) with a voltage of 4.0–6.0 kV was used for the morphology characterization of sensing substrate. Attenuated total reflection Flourier‐transformed infrared (ATR‐FTIR) spectra and Fourier‐transform infrared (FT‐IR) spectra were obtained by a PerkinElmer Frontier with a universal ATR sampling accessory from PerkinElmer (PerkinElmer, Japan). The RGB values were extracted using the software of Adobe Photoshop 2022.
Uhplc q orbitrap hrms
Manufactured by Thermo Fisher Scientific
Sourced in United States
The UHPLC-Q-Orbitrap HRMS is a high-performance liquid chromatography and mass spectrometry system developed by Thermo Fisher Scientific. It combines the capabilities of ultra-high-performance liquid chromatography (UHPLC) and high-resolution Orbitrap mass spectrometry to provide precise, accurate, and sensitive analysis of complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Jsm 7610fplus, by JEOL (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Universal atr sampling accessory, by PerkinElmer (1 mentions)
Q exactive, by Thermo Fisher Scientific (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Frontier ft ir spectrometer, by PerkinElmer (1 mentions)
Jem f200, by JEOL (1 mentions)
D8 advance, by Bruker (1 mentions)
Fls1000 fluorescence spectrophotometer, by Edinburgh Instruments (1 mentions)
Xcalibur 4, by Thermo Fisher Scientific (1 mentions)
4 protocols using uhplc q orbitrap hrms
1
Characterization of Molecular Compounds
2
Comprehensive Characterization of UCNPs
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1H NMR and 13C NMR spectra were obtained on a Varian 400‐NMR spectrometer with CDCl3 or DMSO‐d6 as deuterated solvent. FT‐IR spectra were measured with a PerkinElmer Frontier FT‐IR spectrometer. Mass spectra were obtained on a Q Exactive‐type four stage rod‐Orbitrap high‐resolution mass spectrometer (HRMS, UHPLC‐Q‐Orbitrap‐HRMS, Thermo Fisher Scientific). Fluorescence spectra, UCL spectra and luminescence lifetime of UCNPs were determined by the Edinburgh FLS1000 fluorescence spectrophotometer. UV‐vis absorption spectra were determined with the Hitachi UV‐3900 ultraviolet‐visible spectrophotometer. TEM (JEOL JEM‐F200, Japan) and FE‐SEM (JEOL JSM‐7610F Plus) equipped with an energy‐dispersive X‐ray spectrometer were used for morphology and composition characterization of the UCNPs. The XRD data were measured on the Bruker D8 Advance, with Cu‐Kα radiation operating at 40 kV and 40 mA. XPS spectra were measured on the Thermo Scientific Escalab 250Xi, equipped with an Al Kα (1486.6 eV) X‐ray source. Zeta potential was measured on the Zeta potential analyzer (Malvern Zetasizer Nano ZS90). The optical photographs were taken on a Realme GT Master Explore smartphone, under natural light, 468 nm (FC‐980‐30 W, CNIlaser) and 980 nm (FC‐980‐30 W, CNIlaser) excitation, respectively.
3
Phenolic Profiling of Lyophilized Samples
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To determine phenolic profile and total polyphenols (total phenolic acid derivatives and total flavonoid derivates), 100 mg of each lyophilized sample was mixed with 5.0 mL of methanol/water (60:40 v/v) and sonicated for 30 min at room temperature (Kyriacou et al., 2019). The extraction protocol and the equipment used for the analysis of phenolic compounds was previously described by Kyriacou et al. [4 (link)]. The limit of detection (LOD), limit of quantification (LOQ), linearity of calibration curves, recovery percentage (%), and intra- and inter-day precision of the equipment are presented in Supplementary Material Table S1 .
The phenolic profile was determined using ultrahigh-performance liquid chromatography-quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS; Thermo Fisher Scientific, Waltham, MA, USA) according to the protocol previously described by Petropoulos et al. [37 (link)] and the results are presented as µg g−1 DW.
The phenolic profile was determined using ultrahigh-performance liquid chromatography-quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS; Thermo Fisher Scientific, Waltham, MA, USA) according to the protocol previously described by Petropoulos et al. [37 (link)] and the results are presented as µg g−1 DW.
4
Intracellular Metabolite Extraction and Analysis
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Sample preparation. The mycelia of 5 mL fermentation broth were collected and washed in precooled phosphate-buffered saline (PBS) (KH2PO4 (0.24 g/L), Na2HPO4 (1.44 g/L), NaCl (8 g/L), KCl (0.2 g/L), dissolved in deionized water, pH 7.2). Wet mycelia (1.0 g) were treated with methanol/acetonitrile/H2O (1.5 mL; 40:40:20 v/v/v) at −20 °C for 60 min to extract intracellular metabolites. After freezing and thawing in liquid nitrogen at least five times, extract was centrifuged at 5000× g at 4 °C for 10 min and supernatant was retained to determine metabolite profiles.
Data acquisition and processing. Metabolite determination was carried out using ultra-high-performance liquid chromatography-Q exactive hybrid quadrupole orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap HRMS), as described previously with negative ion mode [36 (link)] in Shanghai Applied Protein Technology Co., Ltd. Xcalibur 4.0 software (Thermo Fisher, Waltham, MA, USA) was used for data acquisition and processing. Identification of metabolites was achieved by high-resolution mass and retention-time matching to authentic standards. Metabolite abundances were normalized to wet mycelium weight. The metabolomics experiment was biologically repeated twice.
Data acquisition and processing. Metabolite determination was carried out using ultra-high-performance liquid chromatography-Q exactive hybrid quadrupole orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap HRMS), as described previously with negative ion mode [36 (link)] in Shanghai Applied Protein Technology Co., Ltd. Xcalibur 4.0 software (Thermo Fisher, Waltham, MA, USA) was used for data acquisition and processing. Identification of metabolites was achieved by high-resolution mass and retention-time matching to authentic standards. Metabolite abundances were normalized to wet mycelium weight. The metabolomics experiment was biologically repeated twice.
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