Cpg c odn m362

TLR ligands: Pam₃CSK4, PGN, LTA, Poly(I:C), CL075, CL097, LPS, Flagellin (FLA-ST), Flagellin (FLA-ST ultrapure grade), R848, CpG-A (ODN2216), CpG-B (ODN2006) and CpG-C (ODN M362) were purchased from Invivogen. Poly (G3) was purchased from Sigma. Mycobacterium tuberculosis (MT) killed bacteria and Bordetella pertussis (BP) inactivated bacteria were purchased from BD Diagnostic Systems. Yellow fever vaccine (YFVAX) was a generous gift from Dr. B. Pullendran, Emory Vaccine Center at Yerkes National Primate Research Center, Emory University, Atlanta, Georgia, USA. ELISA kits for GM-CSF, IL-2, and IL-12 were purchased from R&D systems. Fetal calf serum and human serum were purchased from Gemini. Carboxyfluorescein succinimidyl ester (CFSE) was purchased from Invitrogen. Mouse fibroblast L cell line (L-cell) and OX40 ligand expressing L cells (L-cell/OX40L) generated in our laboratory were maintained with RPMI cultured medium containing 10% fetal calf serum, 1% Glutamax, and 1% penicillin-streptomycin. FL-specific T-cell lines and clones generated from intratumoral T cells were described previously.^{29 (link)}

TLR ligands: Pam₃CSK4, PGN, LTA, poly(I:C), CL075, CL097, LPS-SM, flagellin, CpG-A (ODN2216), CpG-B (ODN2006) and CpG-C (ODN M362) were purchased from Invivogen (San Diego, CA, USA). Phorbol myristate acetate (PMA), ionomycin (ION) and brefeldin A (BFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Elisa kits for IL-2, IL-10, TNF-α and IL-6 and neutralizing antibodies against IL-2 and IL-6, and normal goat IgG control were purchased from R&D Systems (Minneapolis, MN, USA). Fetal calf serum and human serum were purchased from Gemini (Manhattan, New Jersey, USA). Carboxyfluorescein succinimidyl ester (CFSE) was purchased from Invitrogen (Grand Island, NY, USA).