Ugt2b4

Manufactured by Corning

Sourced in United States

UGT2B4 is a laboratory equipment product manufactured by Corning. It functions as an enzyme that catalyzes the conjugation of various lipophilic molecules such as steroids, bilirubin, hormones, and xenobiotics with glucuronic acid. This process is an important step in the detoxification and elimination of these compounds from the body.

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Lab products found in correlation

2 protocols using ugt2b4

In Vitro Enzymatic Stereoselective Metabolism Study

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DIC, MEF, ZOM, FLU, KET, NAP, and protease inhibitor cocktail were purchased from Sigma-Aldrich Co., LLC. Software (St. Louis, MO, USA). The FLU enantiomers (R- and S-FLU), KET enantiomers (R- and S-KET), NAP enantiomers (R- and S-NAP), and DIC-d4 were obtained from Toronto Research Chemicals Inc. (Toronto, Canada). Corning Supersomes Human UGT1A1, UGT1A9, UGT2B4, and UGT2B7 were purchased from Corning, Inc. (Corning, NY, USA). The Pierce BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). RIPA lysis buffer, including protease inhibitors, was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). All other chemicals and solvents were of MS grade or higher and commercially available.

Kawase A., Yamashita R., Yoshizato T., Yoshikawa M., Shimada H, & Iwaki M. (2022). Stereoselective Covalent Adduct Formation of Acyl Glucuronide Metabolite of Nonsteroidal Anti-Inflammatory Drugs with UDP-Glucuronosyltransferase. International Journal of Molecular Sciences, 23(9), 4724.

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In Vitro Licochalcone A Metabolism

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Licochalcone A (E-97%, Z-3% by NMR, Electronic Supplemental Material S1) and β-nicotinamide adenine dinucleotide phosphate, reduced sodium salt (NADPH), uridine 5’-diphosphoglucuronic acid triammonium salt (UDPGA), sulfatase, β-glucuronidase, sulfur trioxide-pyridine complex, and glutathione (GSH) were purchased from Sigma-Aldrich (St. Louis, MO). Pooled human liver microsomes (20 mg/mL, 150 men and women donors), cDNA-expressed human cytochrome P450 1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1, and CYP3A4 (0.5 nmol of P450 in 0.5 mL) and cDNA-expressed recombinant human UGTs including UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT1A10, UGT2B4, UGT2B7, UGT2B15, and UGT2B17 (5 mg protein/mL), were purchased from Corning Life Sciences (Tewksbury, MA). HPLC-grade solvents were purchased from Thermo Fisher (Pittsburgh, PA). Cryopreserved human hepatocytes (50 donors pooled) and manufacturer-specified cell culture media were purchased from Thermo Fisher (Waltham, MA).

Huang L., Nikolic D, & van Breemen R.B. (2017). Hepatic metabolism of licochalcone A, a potential chemopreventive chalcone from licorice (Glycyrrhiza inflata), determined using liquid chromatography-tandem mass spectrometry. Analytical and bioanalytical chemistry, 409(30), 6937-6948.

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