Prism 8 for windows

Estimations of FM, FFM, ALM, and tFM from the validation sample and by the three BIA methods were contrasted with those of DXA as the reference standard by means of Pearson correlation, linear regression, and Lin’s concordance coefficients.
The Bland-Altman method [13 (link)] was used to assess agreement between each BIA method with DXA as the reference standard. The differences of BC values estimated by each BIA device, minus the values estimated using DXA (y-axis), were plotted against the average of said two measurements (x-axis). Differences and limits of agreement were calculated. A linear regression analysis with differences as the dependent variable and the average of measurements as the independent variable was carried out for each BIA method in order to detect if there was a proportional difference [13 (link)].
Statistical analyses were performed using SPSS for Windows version 21.0 (SPSS Inc., Chicago, IL, USA), and Prism 8 for Windows (GraphPad Software, Inc., San Diego, CA, USA). Prediction equations were carried out using R software, version 3.0.1 (The R Foundation for Statistical Computing, Vienna, Austria). Statistical significance was set at p < 0.05 for all analyses.

The results are presented as percentage (e.g., hatchability, recovery of probiotic, mortality etc.) and means with standard errors of mean (SEM) (cfu, body weight). For normalizing cfu counts, log10 transformation was applied. For recovery of probiotic, Prism 8 for Windows (version 8.4.0, GraphPad Software, Inc.) was applied for statistical analysis. Hellinger transformation and subsequent unrestricted principal component analysis of OTU compositions, as well as Kaplan–Meier plots of survival curves followed by comparison by log-rank was done in RStudio Version 1.4.1103 running R version 4.03 (2020–10-10), using the packages: survival (3.2–10) [30 ], survminer (0.4.9) (Kassambara et al., 2021), labdsv (2.0–1) [31 ] and readxl (1.3.1) [32 ]. An alpha level of 0.05 was set. A null-hypothesis stating that groups did not statistically significantly differ was abandoned at P-values < 0.05.

All statistical analyses were performed using GraphPad Prism 8 for Windows, Version 8.2.1 Software (San Diego, CA, USA). Regarding the plasma concentration–time curve, two-way ANOVA followed by Sidak’s multiple comparisons test was conducted to compare each mean with the others at all time points and assess the significance between groups, while a two-tailed unpaired t-test was used to assess the pharmacokinetic parameters of the formulations. Results with p < 0.05 were considered significant.