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2 protocols using hydrogen peroxide 35

1

Synthesis of 4-Nitrophenyl Acetate (4-NPA)

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4-NPA was synthesized as previously reported32 (link). Anhydrous sodium carbonate P.A. (Vetec), Hydrogen Peroxide 35% (CRQ), MO (Sigma-Aldrich), Montmorillonite KSF (Sigma-Aldrich), 4-nitrophenol (Fluka Analytical), Palladium on carbon 9.7% (Sigma-Aldrich), Potassium hydroxide (Neon), Rhodamine B (Sigma-Aldrich), Sodium acetate P.A. trihydrate (Vetec), Sodium borohydride P.S. (Vetec) and Titanium (IV) oxide anatase (Sigma-Aldrich) were purchased with a high grade of purity.
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2

Synthetic Reagents for Protein Modification

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Hydrogen peroxide 35% (H2O2), 2-mercaptoethanol, 4-toluenesulfonyl chloride, potassium
bicarbonate, N-butylamine, triethylamine (TEA), deuterated
solvents, ethyl acetate, cyclohexane, acetonitrile, dimethyl sulfoxide
(DMSO), hydrochloric acid (HCl), sodium bicarbonate (NaHCO3), 2-bromoethanol, N,N-dimethylacrylamide (DMA),
2,2′-azobis(2-methylpropionitrile) (AIBN), bis(pentafluorophenyl)
carbonate, 1,8-diazabicyclo[5.4.0]undec-7-en (DBU), and 1,1′-carbonyldiimidazol
(CDI) were purchased from Sigma-Aldrich. Potassium sulfite, sulfur,
and fluorescamine were purchased from Acros Organics and Alfa Aesar,
respectively. N,N-Dimethylformamide
(DMF), chloroform, dichloromethane (DCM), and the BCA (bicinchoninic
acid) protein assay kit were obtained from Thermo Fisher Scientific
Inc. Methanol (MeOH) and acetone were from Honeywell International.
Vivaspin 500 centrifugal concentrators MWCO 10 kDa were purchased
from Sartorius.
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