Human prostate cancer cell lines (LNCaP, PC-3, and DU145) and human embryonic kidney cell line (HEK-293) purchased from the Korean Cell Line Bank (Seoul, South Korea) were cultured in DMEM/High glucose (DU145) or RPMI1640 (PC-3 and LNCaP) or MEM (HEK-293) supplemented with 10% fetal bovine serum, and maintained in a humidi ed incubator at 37°C in a 5% CO 2 atmosphere.
Hek293
Manufactured by Korean Cell Line Bank
Sourced in United States
HEK293 is a cell line derived from human embryonic kidney cells. It is a commonly used cell line in various research applications, including the study of virus-host interactions, gene expression, and protein production.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (3 mentions)
Du145, by Korean Cell Line Bank (2 mentions)
Hek293, by Welgene (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions)
U87mg, by Korean Cell Line Bank (2 mentions)
Lncap, by Korean Cell Line Bank (1 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)
Ht 29, by Korean Cell Line Bank (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Aspc 1, by Korean Cell Line Bank (1 mentions)
Panc 1, by Korean Cell Line Bank (1 mentions)
Akt and pakt antibodies, by Santa Cruz Biotechnology (1 mentions)
Minimum essential medium (mem), by Welgene (1 mentions)
Hepg2, by Korean Cell Line Bank (1 mentions)
Dulbecco s modified eagle s medium, by Lonza (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
24 protocols using hek293
1
Culturing Human Cell Lines
2
Differentiation and Maintenance of 3T3-L1 and HEK293 Cells
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3T3-L1 and human embryonic kidney 293 (HEK293) cells were purchased from the Korea Cell Line Bank (Seoul, Korea). 3T3-L1 cells were maintained and differentiated as previously described36 (link),37 . Briefly, the cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, Korea) supplemented with 10% calf serum and 1% antibiotics at 37 °C in 5% CO2. Confluent 3T3-L1 cells were incubated for 48 h. Then, the medium was replaced with DMEM supplemented with 10% fetal bovine serum (FBS), dexamethasone (1 µM), insulin (1 µg/ml), and isobutylmethylxanthine (520 µM). After 48 h, the medium was replaced with DMEM supplemented with 10% FBS and insulin (1 µg/ml), and after an additional 48 h, the medium was replaced with DMEM supplemented with 10% FBS. HEK293 cells were maintained in DMEM supplemented with 10% FBS and 1% antibiotics at 37 °C in 5% CO2.
3
Cell Culture of Human CRC Lines
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The human CRC cell lines HT29 and HEK293 were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea), and KM12SM-luc was provided by The University of Texas MD Anderson Cancer Center. HT29, KM12SM-luc, and HEK293 were maintained in DMEM (Welgene, Gyeongsan, Korea) with 10% fetal bovine serum (RMBIO, Missoula, MT, USA) and a 1% antibiotic–antimycotic solution (Thermo Fisher Scientific, Waltham, MA, USA).
4
Cancer Cell Line Characterization
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AGS (gastric cancer), MKN1 (gastric cancer), SNU1197 (colon cancer), AsPC1 (pancreatic cancer), PANC1 (pancreatic cancer), and Hek293 (normal kidney) cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea). Antibiotics (penicillin/streptomycin), fetal bovine serum (FBS), and Roswell Park Memorial Institute (RPMI)-1640 cell culture growth medium were purchased from Gibco (Thermo Fisher Scientific). Bio-Rad Laboratories Inc. Provided the chemicals and materials used for electrophoresis. EZ-CYTOX cell viability, proliferation, and cytotoxicity assay kits were purchased from Do Gen Bio (Korea). Antibodies specific for ERK, pERK, S6, pS6, PARP, and KRAS were acquired from Cell Signaling Technology (Danvers, MA, USA). Santa Cruz provided the AKT and pAKT antibodies and β-actin.
5
Hydrogel Cytotoxicity Evaluation in HEK-293 Cells
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The cytotoxicity of the hydrogels was evaluated using WST-8 assays using human embryonic kidney 239 cells (HEK-293, Korean Cell Line Bank, Korea). For direct cytotoxicity tests, HEK-293 cells were seeded into 24-well culture plates at a concentration of 3 × 104 cells per well with minimum essential medium (MEM, WELGENE, Gyeongsan-si, Korea) containing 10% fetal bovine serum and 1% penicillin/streptomycin; then, 5 mg of hydrogel sample was added to the wells and plates were incubated at 37 °C in an atmosphere containing 5% CO2. MEM medium was used as a negative control, and 10% (v/v) dimethylsulfoxide (DMSO) dissolved in the MEM medium was used as a positive control. After a 48-hour incubation, WST-8 assay reagent (QuantiMax, BIOMAX, Seoul, Korea) was added to each well and absorbance was measured at 450 nm. Cell viability was determined using the following formula:
All assays were repeated in triplicate for each sample.
All assays were repeated in triplicate for each sample.
6
Cell Lines and Animal Models for Drug Metabolism Studies
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Human liver cells HepG2 (KCLB 88065, Korea) and human kidney cells HEK293 (KCLB 21573) were bought from Korean Cell Line Bank (KCLB; Seoul, Korea) and Dulbecco's Modified Eagle's medium (Lonza; Walkersville, MD, USA) containing 10% fetal bovine serum and 1% penicillin-streptomycin (10,000 U penicillin/mL and 10,000 μg streptomycin/mL) was used as culture medium. OAT1- and OAT3-expressing human recombinant CHO-K1 cells and MDR1-expressing Mardin-Darby canine kidney type II (MDR1-MDCKII) cells were cultured in Hanks' balanced salt solution (HBSS)-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH 7.4).
Four-wk-old mice (C57BL/6N, male) were purchased from Orient Bio, Seongnam, Korea. Animal experiments were performed according to the guidelines of the Korean Animal Protection Law.
Four-wk-old mice (C57BL/6N, male) were purchased from Orient Bio, Seongnam, Korea. Animal experiments were performed according to the guidelines of the Korean Animal Protection Law.
7
Characterization of Gastric Cancer Cell Lines and Primary Tissues
8
Cell Culture Conditions for HEK293, A549, and 16HBE14o-
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All cell lines were grown in a 37°C, 5% CO2 environment. Human embryonic kidney 293 cell line (HEK293) and human lung cancer cell line (A549) were obtained from the Korean Cell Line Bank. The human bronchial epithelial cell line (16HBE14o-) was purchased from Merck Millipore. The cells were cultured in Roswell Park Memorial Institute-1640 medium with 0.5 μg/ml puromycin in 10% fetal bovine serum and 1% penicillin/streptomycin.
9
Culturing Human Embryonic Kidney Cells
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The human embryonic kidney cell line (HEK293) was obtained from Korean Cell Line Bank (KCLB, Seoul, Korea). Cells were cultured in Dulbecco’s modified Eagle’s media (Hyclone, Logan, UT, USA) supplemented with 10% fetal bovine serum (Hyclone) and 1% penicillin-streptomycin (Gibco, New York, NY, USA). Cell lines were kept in humidified air containing 5% CO2 at 37 °C.
10
Cell culture protocol for MM and HEK293
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Human MM (RPMI8226, U266) and HEK293 cell lines were obtained from Korea cell line bank (KCLB) and the American Type Culture Collection (ATCC) (Manassas, VA, USA). Cells were cultured in RPMI-1640, DMEM (Gibco BRL, Grand Island, NY, USA) medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco BRL), and antibiotics (100 μg/mL penicillin–streptomycin) (Gibco BRL). Cells were grown and maintained at 37 °C, in a humidified atmosphere of 95% air and 5% CO2.
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