Goat anti rabbit igg h l hrp kit

Hematoxylin and eosin sections representing the tumor of patients diagnosed with gastric adenocarcinoma were re-examined. The best paraffin block was selected for immunohistochemistry staining. Sections with 4-micron thickness were taken from paraffin blocks and transferred onto an adhesive coated slide system. The following method was used for immunohistochemical staining with streptavidin-biotin. Sections were incubated at 56 °C for 12 h for deparaffinization. Three percent hydrogen peroxide was used to block endogenous peroxidase. Antigen retrieval was performed in a microwave oven for 20 min using 0.01 mol/L Tris/EDTA buffer pH 9.0. Sections were coated with primary antibodies including CLDN18.2 (rabbit monoclonal antibody, Clone EPR19202, at 1:500 dilution, Abcam, United Kingdom) and incubated at room temperature for 2 h. Sections were incubated for another 20 min at room temperature after the addition of binding (secondary) antibody (Goat Anti-Rabbit IgG H&L (HRP) kit, Abcam, United Kingdom). The streptavidin-biotin complex was added. 3,3′-Diaminobenzidine was used as chromogen for visualization. CLDN18.2 non-tumor gastric tissues were used as positive controls for each staining session.