Ft ir 6600

Fourier Transform Infrared spectroscopy (FTIR) measurements were performed using a JASCO FT/IR 6600 equipment in two different operation modes: (a) with a specular reflectance standard module RF-81S, scan range 1–1.7 μm with 1 cm⁻¹ resolution using a TGS detector and aperture of 3.5 mm and (b) with an integrated sphere using an InGaAs detector, scan range 0.9–1.7 μm with 4 cm⁻¹ resolution.
Reflection measurements at different incidence angles were performed using a variable angle spectroscopic ellipsometer (Woollam V-VASE). The R_s and R_p data, corresponding to s- and p-polarized incidence waves, were acquired from 0.3 to 1.35 μm with a 0.01 μm step, at three different angles: 35°, 60° and 75°.

X-ray diffraction (XRD) was conducted on a D2 PHASER (Bruker, Germany) and patterns were obtained using CuKα radiation. Fourier transform infrared (FTIR) spectroscopy was conducted by using a Jasco FT/IR-6600. The Brunauer-Emmett-Teller (BET) surface area analysis was conducted on a TriStar II (Micromeritics, GA, USA) was obtained from the N₂ adsorption/desorption isotherm using a fully automatic physisorption analyzer. Scanning electron microscopy (SEM) was conducted on a Nova NanoSEM 450 (FEI, OR, USA) at an acceleration voltage of 5 kV. SEM-energy dispersive spectroscopy (EDS) mapping was performed using a QuantaX200 (Bruker). X-ray photoelectron spectroscopy (XPS, Thermo Scientific, K-Alpha) was conducted using an Al X-ray source. The UV-vis analysis was performed on a V770 spectrophotometer (JASCO). The pH of the solution was measured by using a JENWAY 3510 pH Meter.

In this research, the ability of coumarins to initiate the photopolymerization of (meth)acrylate functions (FRP) was studied using two and three-component photoinitiating systems based on Coum/Iod salt (or NPG) (0.1%/1% w/w) and Coum/Iod/NPG (0.1%/1%/1% w/w/w). The percentage of the different chemical compounds is calculated according to the monomer weight. Kinetic study, as well as the reactive function conversion, were monitored by the evolution of the double bond vs. time. In fact, the polymerization experiments were performed in both thick (1.4 mm) and thin (25 µm) samples, they were obtained by deposition of the formulation into the mold (1.4 mm) or between two propylene films in order to reduce O₂ inhibition, respectively. In addition, excellent solubility of all coumarin derivatives (excluding the CoumE) were observed. For the thick and thin samples, the evolution of the (meth)acrylate functions for TMPTA or Mix-MA were followed by RT-FTIR spectroscopy (JASCO FTIR 6600) at about 6150 cm⁻¹ and 1630 cm⁻¹, respectively. The procedure used to monitor the photopolymerization profile was described in detail in [30 (link),31 (link)].

UV/vis absorption and fluorescence emission measurements were performed using a filter-based multimode microplate reader (Infinite F500; TECAN, Ltd, Männedorf, Switzerland). Transmission electron microscopy (TEM) analysis was carried out using a TEM (JEM-2100F; JEOL, Ltd., Tokyo, Japan) operated at 100 kV. Zeta potential and hydrodynamic particle size were measured by dynamic light scattering (DLS) using a Zetasizer Nano series (Malvern Inst. Ltd., Malvern, UK). Powder X-ray diffraction (PXRD) measurement was carried out using a RINT ULTIMA XRD (Rigaku Co., Tokyo, Japan) with a Ni filter and Cu-Kα source. Data were collected over 2theta = 5–60° at a scan rate of 0.01°/step and 10 s/point. Conjugation of the Ab to the NPs was confirmed via an ELISA and read through a plate reader from Bio-Rad (model 680, Hercules, USA). Fourier transform infrared spectroscopy was achieved using the FT/IR-6600 (JASCO, Tokyo, Japan).