On arrival at the scanning facility, sheep were sedated by intravenous injection of 0.5 mg/kg live weight diazepam (Troy Laboratories NZ Pty Ltd, Auckland, NZ) and 10 mg/kg live weight ketamine (Phoenix Pharm Distributors Ltd, Auckland, NZ) prior to endotracheal intubation and maintenance on inhalation anaesthesia (isoflurane in oxygen, 1.5–3% v/v to effect).
Structural imaging was performed to measure tissue volumes using a T1-weighted magnetization-prepared rapid gradient-echo sequence: echo time/repetition time = 2.02/2500 ms, inversion time = 900 ms, flip angle = 12deg, Field of view = 192 mm, slice thickness = 0.75 mm, matrix = 256 × 256, voxel size = 0.75 × 0.75 × 0.75 mm3, NEX = 4, scan time =25:32 min and slice acceleration GRAPPA = 2.