In this work, various materials were used. These materials are 50 polypropylene conical tubes (Baxter, Toronto, ON, Canada); Sterile cell strainer: 40 μm nylon mesh (Millipore, Burlington, MA, USA); 11 μm nylon filters (Millipore, Burlington, MA, USA); 1.5 mL Eppendorf tubes (Fisherscience, Saint-Laurent, QC, Canada); 0.9% irrigation-grade sodium chloride solution (Baxter, Toronto, ON, Canada). We also employed several instruments, including an incubator (Heracell 150i, Thermo Fisher Scientific, Waltham, MA, USA); Lab Centrifuge (Sorvall ST 8, Thermo Scientific, Waltham, MA, USA); Hemocytometer (BLAUBRAND® Neubauer Millipore Sigma, Burlington, MA, USA); Inverted and phase contract Microscope (isherbrandTM Inverted Infinity, Phase contrast 10× and 20×, light splitter (100% or 20/80%), Fisher Scientific, Hampton, NH, USA).
Lab centrifuge
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
A lab centrifuge is a device used to separate different components of a liquid mixture based on their densities. It works by spinning the sample at high speeds, which creates a centrifugal force that causes the denser components to move to the bottom of the container while the lighter components remain at the top. This process allows for the efficient separation and isolation of various substances, such as cells, proteins, or other biomolecules, for further analysis or processing.
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Lab products found in correlation
2 protocols using lab centrifuge
1
Polypropylene Tube Cell Isolation Protocol
2
Solubility Assessment of Piroxicam-β-Cyclodextrin
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Solubility studies were performed using different solvents. Piroxicam-β-cyclodextrin was added in sufficient amount to 50 mL of various solvents. Then, these solutions were stirred for 24 hours at 37 ± 0.5°C. After stirring, the samples were centrifuged (heraeus megafugr 8R; Thermofisher Scientific Lab Centrifuge, Zweigniederiassung Osterode, Osterode am Harz, Germany) for 10 minutes at 4193 × g to eliminate the extra quantity of piroxicam-β-cyclodextrin. The supernatant layer was filtered and properly diluted with respective solvents. The concentration of piroxicam-β-cyclodextrin was determined spectrophotometrically (O.R.1 3000, UV–visible spectrophotometer, Iba, Göttingen, Germany) at 207 nm using specific solvent as a blank [20 ].
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