Four milliliters of venous blood (2 ml preoperative and 2 ml postoperative) were collected into an EDTA test tube from each study participant by Medical Laboratory Science professionals. Hemoglobin concentration was determined using UniCel DxH 800 (Beckman Coulter, USA) automated hematology analyzer by spectrophotometry method15 (link). Hemoglobin concentration was analyzed at the hospital hematology laboratory and recorded for each study participant using a laboratory result registration form.
Unicel dxh 800
Manufactured by Beckman Coulter
Sourced in United States
The UniCel DxH 800 is a compact, fully automated hematology analyzer developed by Beckman Coulter. It is designed to perform complete blood count (CBC) and white blood cell differential analysis. The instrument utilizes advanced technology to provide accurate and reliable results for clinical diagnostic purposes.
Automatically generated - may contain errors
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Au5800 analyzer, by Beckman Coulter (1 mentions)
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Au5800 chemistry analyzer, by Beckman Coulter (1 mentions)
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39 protocols using unicel dxh 800
1
Hemoglobin Concentration Measurement Protocol
2
Hematological Biomarkers at Diagnosis
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Blood values had been taken into consideration at the time of diagnosis before administration of any treatment when patients were admitted to the hospital. Venous blood specimens were drawn into sterile standard tubes containing ethylene diamine tetraacetic acid (EDTA) as an anticoagulant and evaluated within 1 h after venipuncture using a Beckman Coulter UniCel DxH800 hematology analyzer. PC, RDW, WBC, MPV, NLR, and PLR values were then obtained directly from the routine blood tests through the medical database.
3
Comprehensive Metabolic and Hormonal Profiling
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Fasting venous blood samples of the subjects were collected and centrifuged at 3500 rpm for 10 minutes at room temperature. The samples were stored at −80°C until being analyzed. Blood cell analysis was measured using an automatic blood analyzer (UniCelDxH 800, BECKMAN, USA). Serum levels of follicle-stimulating hormone, LH, estradiol, progesterone, total testosterone, dehydroepiandrosterone sulfate, thyroid-stimulating hormone, fasting insulin (FINS), fasting plasma glucose (FPG), and hs-CRP were measured using commercially available kits on an autoanalyzer by enzyme chemiluminescent immunoassay (ADVIA1800, Siemens, Germany). Insulin resistance was calculated by the homeostasis model assessment-insulin resistance (HOMA-IR; FPG [mmol/L] × FINS [μIU/mL]/22.5).
4
Automated Hematology Analyzer Evaluation
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UniCel DxH800 (Beckman Coulter) automates measured hemoglobin level, MCV, MRV, MSCV, delta (MCV‐MSCV), IRF (the count of reticulocytes with the highest RNA). The instruments were regularly controlled by internal and external quality controls, and their analytical variability was lower than 1% for all considered parameters.
5
Biomarker Analysis of Fasting Blood
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Fasting venous blood samples were collected from all subjects and centrifuged at 3500 rpm for 10 minutes at room temperature. Samples were stored at –80°C until analysis. Blood cell analysis was performed using an automatic blood analyzer (UniCelDxH 800, BECKMAN). Serum levels of HCY, CRP, and lipid indices were measured using commercially available kits on an autoanalyzer by enzyme chemiluminescent immunoassay (Siemens Healthcare Diagnostics, ADVIA1800, Siemens AG, Germany).
6
Comprehensive Blood Cell and Metabolic Analysis
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RDW measurement was performed with peripheral venous blood using an automated complete blood cell counter (UniCel DxH 800, Beckman Coulter Inc., Miami, FL, USA), which simultaneously provides values for hemoglobin, MCV, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count, and total white blood cell count. Other collected laboratory data are total cholesterol, triglyceride, glucose, and estimated glomerular filtration rate (eGFR). The eGFR was calculated using the abbreviated Modification of Diet in Renal Disease Study equation [186 x Serum Creatinine-1.154 x Age-0.203 x (0.742 if female)] [18 (link)].
7
Structured Questionnaire and Blood Analysis Protocol
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An interviewer-administered structured questionnaire, adapted from the WHO STEPS instrument,23 was used to collect socio-demographic profiles, behavioral related factors, and clinical data of eligible participants.
After the interview and detailed review of the medical record, 5mL of the venous blood sample was collected from each eligible study participant using 5-cc sterile syringes, through the aseptic technique. About 2.5 mL of blood was dispensed into a labeled test tube containing ethylene-diamine-tetra acetic acid (EDTA) anticoagulant for testing of RBC parameters. The remaining 2.5 mL of blood was collected in a separate EDTA test tube for the analysis of HbA1c. The specimen was then transported to JMC laboratory unit for analysis on the same date of specimen collection to prevent whole blood hemolysis. RBC parameters were analyzed by a fully automated hematology analyzer, UniCel DxH 800 (Beckman Coulter, USA). HbA1c was determined by a fully automated Cobas® 6000 chemistry analyzer (Roche Diagnostics, Germany).
After the interview and detailed review of the medical record, 5mL of the venous blood sample was collected from each eligible study participant using 5-cc sterile syringes, through the aseptic technique. About 2.5 mL of blood was dispensed into a labeled test tube containing ethylene-diamine-tetra acetic acid (EDTA) anticoagulant for testing of RBC parameters. The remaining 2.5 mL of blood was collected in a separate EDTA test tube for the analysis of HbA1c. The specimen was then transported to JMC laboratory unit for analysis on the same date of specimen collection to prevent whole blood hemolysis. RBC parameters were analyzed by a fully automated hematology analyzer, UniCel DxH 800 (Beckman Coulter, USA). HbA1c was determined by a fully automated Cobas® 6000 chemistry analyzer (Roche Diagnostics, Germany).
8
Anthropometric and Hematological Evaluation of Diabetic Patients
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Socio-demographic data like age, sex, residence, occupation, and educational status were collected by a pretested structured questionnaire. Duration of diabetes and fasting blood sugar levels of the previous two months were tracked review of the patient’s medical records. The anthropometric data such as height, weight, and waist circumference (WC) were collected by following the anthropometric measurement protocol. Body mass index (BMI) was determined as weight in kilograms divided by height in meter squared.
Five milliliters of venous blood sample (2mL in a serum separator tube and 3mL in a K2EDTA tube) were collected using a vacutainer blood collection system from each diabetic patient. Again, three milliliters of venous blood were collected into a K2EDTA test tube from each control group. Fasting blood glucose was estimated from a serum separator tube by glucose oxidase method,25 using Biosystems A25 (Costa Brava, Spain) clinical chemistry analyzer. Red blood cell parameters were determined by UniCel DxH 800 (Beckman Coulter, USA) hematology analyzer by following the electrical impedance and spectrophotometry principle.26
Five milliliters of venous blood sample (2mL in a serum separator tube and 3mL in a K2EDTA tube) were collected using a vacutainer blood collection system from each diabetic patient. Again, three milliliters of venous blood were collected into a K2EDTA test tube from each control group. Fasting blood glucose was estimated from a serum separator tube by glucose oxidase method,25 using Biosystems A25 (Costa Brava, Spain) clinical chemistry analyzer. Red blood cell parameters were determined by UniCel DxH 800 (Beckman Coulter, USA) hematology analyzer by following the electrical impedance and spectrophotometry principle.26
9
Plasma Analyte Profiling Protocol
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Blood samples were centrifuged at 800xg at 4°C for 10 min in a bench centrifuge. Supernatant (plasma) was aliquoted and stored on dry ice until all samples were frozen at -80°C. Sodium and potassium plasma concentrations were determined using ion selective electrodes with an ionized sodium/potassium analyzer KNA1 (Radiometer, Copenhagen, Denmark). Creatinine and urea were measured using an AU5800 analyzer (Beckman Coulter, Hospitalet de Llobregat, Spain). C-reactive protein was determined using the AU-5800 Chemistry Analyzer (Bekman Coulter, Miami, FL, USA). Complete blood counts were performed with the Unicel DxH800 automated hematology analyzer (Beckman Coulter, Miami, FL, USA).
10
Comprehensive Cardiovascular Biomarker Analysis
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The complete blood counts were performed on the Unicel DxH800 automated hematology analyzer (Beckman Coulter, Miami, FL, USA). N-terminal pro-B-type natriuretic peptide (NT-proBNP) was quantified in whole EDTA blood using the AQT90 FLEX immunoassay (Radiometer Medical, Copenhagen, Denmark). Serum creatine kinase (CK) and C-reactive protein (CRP) were determined using the AU-5800 Chemistry Analyzer (Beckman Coulter). Troponin T was measured from serum, using a High Sensitive Troponin-T assay in a Cobas e601 platform (Roche Diagnostics, Barcelona, Spain). ST2 was measured from serum samples using a high-sensitivity sandwich monoclonal immunoassay (Pressage® ST2 assay, Critical Diagnostics, San Diego, CA, USA).
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