Hb 201

Manufactured by HemoCue

Sourced in Sweden, United States, Germany

The HemoCue Hb 201 is a portable hemoglobin analyzer that provides quick and accurate measurements of hemoglobin levels in whole blood samples. The device uses a microcuvette system to analyze the blood sample and displays the hemoglobin concentration.

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Lab products found in correlation

Osmomat 030, by Gonotec (2 mentions) Glucose 201, by HemoCue (2 mentions) Whatman, by Cytiva (2 mentions) Elisas, by R&D Systems (1 mentions) 3d3 osmometer, by Advanced Instruments (1 mentions) Advanced 3d3 osmometer, by Advanced Instruments (1 mentions) Dash 2000, by GE Healthcare (1 mentions) Cobas c501, by Roche (1 mentions) Vitros immuno diagnostic kit, by Ortho Clinical Diagnostics (1 mentions) Onyx 9590, by Nonin Medical (1 mentions) Pal 10s, by Atago (1 mentions) Tim trio system, by Siemens (1 mentions) G6888, by Trinity Biotech (1 mentions) G5888, by Trinity Biotech (1 mentions) Whatman 903, by GE Healthcare (1 mentions) Microcuvette, by HemoCue (1 mentions) Advia 2120i hematology system, by Siemens (1 mentions) Vacutainer blood collection tube, by BD (1 mentions) Bc 6800plus, by Mindray (1 mentions) Xn 3000, by Sysmex (1 mentions)

Close spelling variants of this product

HemoCue® Hb 201+ System (29 citations) HemoCue Hb 201+ analyzer (27 citations) HemoCue Hb 201+ analyser (9 citations) Hb 201+ model (7 citations) HemoCue Hb 201 microcuvette (6 citations) HemoCue Hb 201+ machine (4 citations) HemoCue® Hb 201+ photometer (4 citations) Hb 201 DM (2 citations)

62 protocols using hb 201

Evaluating Intestinal and Inflammatory Markers Post-Exercise

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Posture-controlled venous blood (~20 mL) was collected without stasis before exercise; and 20 min, 60 min and 240 min after exercise. Samples were drawn into sterile syringes and immediately transferred to chilled citrate (5 mL, Sarstedt, Leicester, UK), ethylenediaminetetraacetic acid (EDTA, 5mL; Sarsetdt, Leicester, UK) or pre-warmed (37°C)
Accepted version International Journal of Sports Nutrition and Exercise Metabolism, 31/01/2022 8 EDTA tubes (10 mL). Haematocrit was determined from microcapillary tubes that were loaded in triplicate, and haemoglobin was assayed using microcuvettes (Hb 201, Hemocue®, Äbgelholm, Sweden) and a Hemocue photometer (Hb201+, Hemocue®, Äbgelholm, Sweden). Plasma volume changes were calculated from hematocrit and haemoglobin (Dill & Costill, 1974) , and circulating measures of IFABP, LBP, sCD14, IL6, IL-10, and IL-1RA adjusted accordingly. I-FABP concentrations were measured as a marker of intestinal damage using a commercially available ELISA (Hycult Biotech, USA). LBP, sCD14, IL-6, IL-10 and IL-1RA were analysed in duplicate using ELISAs from R&D Systems. Inter and intra assay coefficient of variations were below 5%, except for the inter-assay CV for IL-6 (6.1%). All samples were assayed in duplicate.

Lee B.J., Flood T.R., Hiles A.M., Walker E.F., Wheeler L.E.V., Ashdown K.M., Willems M.E.T., Costello R., Greisler L.D., Romano P.A., Hill G.W, & Kuennen M.R. (2022). Anthocyanin-Rich Blackcurrant Extract Preserves Gastrointestinal Barrier Permeability and Reduces Enterocyte Damage but Has No Effect on Microbial Translocation and Inflammation After Exertional Heat Stress. International journal of sport nutrition and exercise metabolism, 32(4).

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Assessing Respiratory and Hemodynamic Parameters

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VC (in ml; high precision digital electronic spirometer, Donghuateng Sports Apparatus Ltd, Beijing, CN) and hemoglobin concentration [Hb] (in mg per l; HemoCue Hb 201; HemoCue AB, Angelholm, Sweden) were diagnosed by medical personnel of the Shanghai University of Sport. The typical error for the expiratory VC measurement is 1.7% [46 (link)]. Arterialized blood samples were taken from a hyperemized earlobe to determine the [Hb]. [Hb] was assessed photometrically (HemoCue® HB201+, HemoCue AB, Sweden). The typical error for [Hb] measurement with this device is as accurate as CV < 1.0% (limits of agreement –1.28 to 0.20 gr/dL; [47 (link)]). The HR at rest was recorded in all five periodical measurements for 2 min immediately after awakening in the morning by positioning an H7 Bluetooth HR strap (Polar Electro, Kempele, Finland) on the chest.

Zhao K., Hohmann A., Faber I., Chang Y, & Gao B. (2020). A 2-year longitudinal follow-up of performance characteristics in Chinese male elite youth athletes from swimming and racket sports. PLoS ONE, 15(10), e0239155.

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Blood Hemoglobin Measurement Using HemoCue

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Blood hemoglobin level was measured using HemoCue Hb201⁺. To measure blood hemoglobin level, the patient's forefinger was prepared by wiping the tip with alcohol to sterilize the area and then allowed to dry. The sterilized area was then pricked with a lancet and a drop of blood collected into a microcuvette. The microcuvette filled with the blood was then inserted into the HemoCue Hb201⁺ for the hemoglobin measurement. The measured hemoglobin was classified as normal (Hb > 13 g/dL for men and Hb > 12 g/dL for women), mild anemia (Hb: 11.0–12.9 g/dL for men versus Hb: 11.0–11.9 g/dL for women), moderate anemia (Hb: 8.0–10.9 g/dL), and severe anemia (Hb < 8 g/dL).

, & Anderson A.K. (2017). Prevalence of Anemia, Overweight/Obesity, and Undiagnosed Hypertension and Diabetes among Residents of Selected Communities in Ghana. International Journal of Chronic Diseases, 2017, 7836019.

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Quantification of Cerebral Metabolic Rate of Oxygen

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Quantification of CMRO₂ is based on conservation of mass (Fick’s Principle): CMRO₂ = Ca · BFR/mass · (SaO₂ − SvO₂). Ca represents the oxygen carrying capacity of arterial blood and is defined as Ca = C_RBC · hematocrit, with C_RBC as the oxygen carrying capacity of red blood cells (C_RBC = 22.02 μmol O₂/mL RBC (15 (link))). Hematocrit was determined via a capillary blood sample (Hb 201+, Hemocue, Angelholm, Sweden). Brain volume was calculated from T₁-weighted MPRAGE images (16 (link)) using SPM12 (Wellcome Centre for Human Neuroimaging, University College London, UK) and converted to brain mass by assuming a tissue density of 1.05g/mL (17 ). SaO₂ was assumed to be 98%, since all subjects were healthy participants.

Deshpande R.S., Langham M.C., Cheng C.C, & Wehrli F.W. (2022). Metabolism of Oxygen via T2 and Interleaved Velocity Encoding (MOTIVE): a rapid method to quantify whole-brain cerebral metabolic rate of oxygen. Magnetic resonance in medicine, 88(3), 1229-1243.

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Arsenic Determination in Drinking Water

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Nitric acid, perchloric acid, sodium borohydride, potassium iodide, sodium hydroxide, hydrochloric acid, and ascorbic acid were purchased from J.T Baker (Avantor Performance Materials, Ecatepec, Estado de Mexico, Mexico) and Fermont (Rye S.A de C.V, Tlalnepantla, Estado de Mexico, Mexico). The microcuvettes for hemoglobin determination (Hb-201) were purchased from HemoCue (HemoCue, Ciudad de Mexico, Mexico). The TBARS assay kit (MDA) was purchased from Cayman Chemical (Cayman Chemical, Ann Arbor, MI, USA). The arsenic standard was purchased from Agilent (Agilent, Santa Clara, CA, USA). The quality control for As (ClinChek^®-urine control lyophilised for trace elements, level II) was purchased from Iris Tech (IRIS Technologies International GmbH, Olathe, KS, USA). The concentration of arsenic (As) in drinking water with Arsenator^®-Digital Arsenic Test Kit from Wagtech WTD, Palintest, CO, USA). All solutions were prepared using deionized water.

Santiago-Saenz Y.O., Monroy-Torres R., Rocha-Amador D.O, & Hernández-Fuentes A.D. (2019). Effect of a Supplementation with Two Quelites on Urinary Excretion of Arsenic in Adolescents Exposed to Water Contaminated with the Metalloid in a Community in the State of Guanajuato, Mexico. Nutrients, 12(1), 98.

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Fluid and Electrolyte Regulation in Aging

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Participants laid in the supine position for ≥20 minutes prior to venous blood sample collection. Paired resting biochemical data are reported from all seven older adults and five of eight young participants due to difficulties collecting blood samples. Venous blood samples and 24‐hour urine samples were analyzed for serum and urine electrolyte concentrations (EasyElectrolyte Analyzer; Medica, Bedford, MA, USA), as well as plasma and urine osmolality (3D3 Osmometer; Advanced Instruments, Norwood, MA). Venous blood samples were also analyzed for Hb (Hb 201+; HemoCue, Lake Forest, CA, USA) and Hct (Pre‐calibrated Clay Adams, Readacrit Centrifuge; Becton Dickinson, Sparks, MD, USA). Change in plasma volume (expressed as a percentage) was calculated using the following equation (Dill & Costill, 1974):

Plasma volume (%) = ((100 \times ({Hb}_{CON} / {Hb}_{WD})) \times (1 ‐ ({Hct}_{WD} / 100))) / ((1 ‐ ({Hct}_{CON} / 100)) ‐ 100) .

Urine specific gravity was determined from the spot and 24‐hour urine samples. Young female participants’ spot urine samples were also used to confirm that they were not pregnant (hCG cassettes, Moore Medical).

Watso J.C., Robinson A.T., Babcock M.C., Migdal K.U., Witman M.A., Wenner M.M., Stocker S.D, & Farquhar W.B. (2020). Short‐term water deprivation attenuates the exercise pressor reflex in older female adults. Physiological Reports, 8(18), e14581.

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Fasting Biomarker Assessment Protocol

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Experimental visits were scheduled in the morning (7 am–10 am) of Day 10 and at the same time for both conditions. Participants were asked to fast for at least 9 h, avoid caffeine and alcohol for at least 12 h, and not exercise for 24 h. Upon arrival, body mass was measured. After 5 min of rest, seated BP was assessed in triplicate and averaged (dominant arm; Dash 2000, GE medical systems, Chicago, IL). A venous blood sample was collected to assess hematocrit (Thermo Sorvall), hemoglobin (Hb 201+, HemoCue, Angelholm, Sweden), serum electrolytes (EasyElectrolyte Analyzer, Medica, Bedford, MA), plasma osmolality (Advanced 3D3 Osmometer, Advanced Instruments, ON, Canada), and the lipid profile (LabCorp).

Ramos Gonzalez M., Axler M.R., Kaseman K.E., Lobene A.J., Farquhar W.B., Witman M.A., Kirkman D.L, & Lennon S.L. (2023). Melatonin supplementation does not alter vascular function or oxidative stress in healthy normotensive adults on a high sodium diet. Physiological Reports, 11(24), e15896.

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Plasma Volume and Hormonal Analysis

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Whole-blood hemoglobin (Hb) (Hb201+, Hemocue AB, Ängelholm, Sweden), and hematocrit (HCT) (centrifuged capillary tubes, Propper, Long Island City, USA) were used to calculate changes in plasma volume (Pv) relative to baseline, and to correct plasma variables (Dill & Costill, 1974) . Remaining blood samples were centrifuged at 4000 RPM and 4 o C for 10 min, within 15 min of collection. Plasma was aliquoted into 1.7 mL microstorage tubes and frozen at -80 °C until analysis, except for 100 μL (2 x 50 μL) that was used to determine plasma osmolality (POsm), in duplicate (CV 0.8%), by freeze-point osmometry (Osmomat 030; Gonotec, Berlin, Germany). Plasma [Na + ] was determined using ion selective electrodes (Cobas c 501, Roche Diagnostics, Risch-Rotkreuz, Switzerland) and analysed by local pathology services (Cabrini Pathology, Malvern, Victoria, Australia).
Plasma aldosterone (DE5298; Demeditec Diagnostics GmbH, Kiel, Germany) and cortisol (RE52061; IBL International, Hamburg, Germany) were determined by ELISA. All variables were analysed as per manufacturer's instructions on the same day, with standards and controls on each plate, and each participant's samples on the same plate. Aldosterone and cortisol CV's were 3.9% and 5.8%, respectively.

McCubbin A.J., Lopez M.B., Cox G.R., Caldwell Odgers J.N, & Costa R.J.S. (2019). Impact of 3-day high and low dietary sodium intake on sodium status in response to exertional-heat stress: a double-blind randomized control trial. European journal of applied physiology, 119(9).

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Enrollment Criteria for Child Malaria Study

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The average number of children ≤ 5 years of age in each cluster was 68 (57–79). From individual households in study clusters, one child, aged ≥ 6 months to ≤ 5 years at the time of recruitment, was provided the opportunity to enroll as a subject in the study. Following informed consent, medical screening consisted of physical examination by a study physician and a qualitative nicotinamide adenine dinucleotide phosphate (NADPH) spot test for G6PD deficiency (Trinity Biotech qualitative glucose-6-phosphate dehydrogenase (G6PD) assay, ref 345-UV, Trinity Biotech, St. Louis, MO).^{38 (link)} In addition to being of G6PD normal status, eligibility requirements included bodyweight ≥ 40 kg, hemoglobin > 5 mg/dL (Hb201+, HemoCue AB, Angelholm, Sweden),^{39 (link)} no severe acute illness/infection on the day of inclusion, temperature ≤ 38°C, participant acknowledging sleeping in the village > 90% of nights during any given month, not participating in another clinical trial, and no intensions for extended travel during the study period.

Syafruddin D., Asih P.B., Rozi I.E., Permana D.H., Nur Hidayati A.P., Syahrani L., Zubaidah S., Sidik D., Bangs M.J., Bøgh C., Liu F., Eugenio E.C., Hendrickson J., Burton T., Baird J.K., Collins F., Grieco J.P., Lobo N.F, & Achee N.L. (2020). Efficacy of a Spatial Repellent for Control of Malaria in Indonesia: A Cluster-Randomized Controlled Trial. The American Journal of Tropical Medicine and Hygiene, 103(1), 344-358.

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Biomarkers in High Altitude Miners

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Blood samples were drawn from participants on any day of their respective shift (day or night) at the mine health facility in their workplace, under fasting conditions and standardized procedures, described in the first year of the follow-up study (23 ). The serum was obtained by centrifugation and kept at 4 °C for transporting to the Laboratory of Universidad de Chile Clinical Hospital (Santiago city). Then, all samples were stored at −80 °C before analysis.
High altitude biomarkers such as the hemoglobin oxygen saturation (SaO₂) and hemoglobin (Hb) were measured in situ by pulse oximeter Onyx 9590 (Nonin Medical Inc, Minneapolis, USA) and hemoglobin meter HB201 (HemoCue AB, Angelholm, Sweden), respectively. Levels of PSA and testosterone were analyzed by VITROS immunodiagnostic kit (Ortho Clinical Diagnostics, Bridgend, UK). The symptoms of acute mountain sickness were also asked through the Lake Louise score, with a cut-off above 3 points (24 (link)). These symptoms (25 ) are frequent on the first day of the high-altitude shift, after returning from low-altitude rest days.

Alcantara-Zapata D.E., Bangdiwala S.I., Jimenez D., Kogevinas M., Marchetti N, & Nazal C.N. (2021). EFFECTS OF CHRONIC INTERMITTENT HYPOBARIC HYPOXIA ON PROSTATE-SPECIFIC ANTIGEN (PSA) LEVELS IN CHILEAN MINERS. Occupational and environmental medicine, 78(10), 753-760.

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