3 3 5 5 tetramethyl benzidine tmb

CTAC, TEA, xanthine, and xanthine oxidase (XO) were purchased from Sigma-Aldrich. TEOS and NaOH were obtained from Shanghai Lingfeng Chemical Reagent Co., Ltd. Fe(acac)₃ was provided by J&K Scientific. Urea, RhB, iron chloride hexahydrate (FeCl₃·6H₂O) and 3,3′,5,5′-tetramethyl-benzidine (TMB) were bought from Sinopharm Chemical Reagents Co., Ltd. Methoxy PEG silane was purchased from Shanghai Yare Biotech, Co., Ltd. Gallic acid was acquired from Macklin. Dulbecco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin, and PBS were provided by Gibco. FITC, 4′, 6-diamidino-2-phenylindole (DAPI), DCFH-DA, CCK-8 assay, calcein-AM, PI, annexin V-FITC, H&E, paraformaldehyde (PFA) and TUNEL apoptosis assay kit were acquired from Beyotime. Primary antibody against GPX4 and a biotinylated secondary antibody (anti-rabbit IgG, HRP-linked antibody) were purchased from Cell Signaling Technology.

Sodium hydroxide, hydrogen peroxide, disodium hydrogen phosphate dodecahydrate, sodium chloride and 3,3′,5,5′-tetramethylbenzidine (TMB) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Horseradish peroxidase was purchased from Hubei Xinxin Belle Biotechnology Co., Ltd. (Wuhan, China). Potassium tetrachloroaurate (III) was purchased from Sahn Chemical Technology Co., Ltd. (Shanghai, China). Cucurbit[6]uril and cucurbit[7]uril were synthesized by our group [14 (link),17 (link)].