Materials were obtained from the following suppliers: antibodies against XBP‐1, GAPDH, and CD31 were obtained from Santa Cruz biotechnology Inc. (Santa Cruz, CA); BrdU cell proliferation ELISA kit was purchased from Roche (Penzberg, Germany); CCK‐8 kit was obtained from Beyotime Institute of Biotechnology (Nantong, China); pCMV6‐XL5‐XBP‐1s plasmid was purchased from Origene (Rockville, MD); Inhibitor, mimic, and non‐specific negative control oligonucleotides for hsa‐miR‐214, specific siRNA against human XBP‐1 were obtained from RiboBio (Guangzhou, China). All other chemicals and reagents were purchased from Sigma–Aldrich (Shanghai, China) unless otherwise specified.
Pcmv6 xl5 xbp 1s plasmid
Manufactured by OriGene
Sourced in China
PCMV6-XL5-XBP-1s plasmid is a laboratory tool for studying the expression of the XBP-1s gene. It is a plasmid vector that contains the coding sequence for the spliced form of the X-box binding protein 1 (XBP-1s) under the control of a cytomegalovirus (CMV) promoter.
Automatically generated - may contain errors
2 protocols using pcmv6 xl5 xbp 1s plasmid
1
Evaluating XBP-1 Regulation in Cell Proliferation
2
Exploring XBP-1 Signaling Pathways
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