Lncap

A human monocyte cell line, THP-1, was purchased from the Korean Cell Line Bank (KCLB) (Seoul, South Korea) and cultured in RPMI medium containing 10% fetal bovine serum and 1% antibiotics (Welgene Inc., Daegu, South Korea) in a humidified atmosphere at 37 °C and 5% CO₂. Murine macrophage RAW 264.7 and the human immortalized keratinocyte cell line HaCaT were purchased from KCLB (Seoul, South Korea) and cultured in DMEM containing 10% fetal bovine serum and 1% antibiotics (Welgene Inc., Daegu, South Korea) in a humidified atmosphere at 37 °C and 5% CO₂. A human immortalized sebocyte cell line, SEB-1, was purchased from Celprogen (Torrance, CA, USA) and cultured in EpiLife Medium (Invitrogen-Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum, 1% antibiotics (Welgene Inc., Daegu, South Korea), and 5 ng/mL epidermal growth factor in a humidified atmosphere at 37 °C and 5% CO₂. A human prostate cancer cell line, LNCaP, was purchased from the ATCC. The LNCaP cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% antibiotics (Welgene Inc., Daegu, Republic of Korea) in a humidified atmosphere at 37 °C and 5% CO₂.

Human prostate cancer cells DU145, PC3, LNCaP and human normal prostate epithelial RWPE1 cells were bought from American Type Culture Collection (ATCC). DU145, PC3 and LNCaP cells were maintained in RPMI1640 with 10% FBS and 1% antibiotic (Welgene, Gyeongsan-si, Korea). RWPE-1 cells were cultivated using Keratinocyte Serum-Free Medium supplemented with 0.05 mg/mL bovine pituitary extract, 5 ng/mL recombinant human epidermal growth factor (EGF) and 1% antibiotic (Invitrogen, Grand Island, NY, USA).

Human prostate cells lines, PC-3, LNCaP and RWPE-1, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Prostate cancer PC-3 cells and LNCaP cells were cultured in Dulbecco’s modified Eagle’s minimal medium (DMEM, WelGENE, Daejon, South Korea) and Roswell Park Memorial Institute (RPMI) 1640 (WelGENE, South Korea) supplemented with 10% FBS and 1% penicillin-streptomycin solution with 5% CO₂ at 37°C, respectively. Normal prostate epithelial RWPE-1 cells were cultured in keratinocyte serum-free media (K-SFM) containing 2.5 μg of epidermal growth factor (EGF), 25 mg of bovine pituitary extract (BPE, Invitrogen, Carlsbad, CA, USA) and 1% penicillin-streptomycin solution with 5% CO₂ at 37°C.

Human prostate cell lines, PC-3, LNCaP and RWPE-1 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Prostate cancer PC-3 and LNCaP cells were cultured in Dulbecco’s modified Eagle’s minimal medium (DMEM, WelGENE Inc., Korea) and Roswell Park Memorial Institute (RPMI) 1640 (WelGENE Inc., Korea) supplemented with 10 % FBS and 1 % penicillin-streptomycin solution with 5 % CO₂ at 37 °C, respectively. Prostate normal RWPE-1 cells were cultured in keratinocyte serum-free media (K-SFM) containing 2.5 μg of epidermal growth factor (EGF), 25 mg of bovine pituitary extract (BPE, GIBCO) and 1 % penicillin-streptomycin solution with 5 % CO₂ at 37 °C.