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Bead recovery reagents kit

Manufactured by Roche
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Sourced in United States

The Bead Recovery Reagents Kit is a collection of reagents designed to facilitate the recovery of magnetic beads from a sample. The kit includes the necessary components to efficiently separate and isolate the magnetic beads from the surrounding matrix, enabling their subsequent use in various downstream applications.

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Lab products found in correlation

Oil and breaking kit, by Roche (3 mentions) Gs junior titanium empcr kit, by Roche (2 mentions) Gs junior bead counter, by Roche (2 mentions) Picotiterplate, by Roche (2 mentions) Gs titanium sequencing kit, by Roche (2 mentions) Gs junior device, by Roche (2 mentions) Magnetic particle collector, by Thermo Fisher Scientific (2 mentions) Gs junior system, by Roche (2 mentions) Gs junior titanium empcr kit lib l, by Roche (1 mentions) Lib l kit, by Roche (1 mentions)

3 protocols using bead recovery reagents kit

1

Emulsion PCR and Bead Enrichment Protocol

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Emulsion PCR, oil breaking, and bead enrichment were performed using the GS Junior Titanium emPCR Kit (Lib-A), emPCR Reagents Lib-A kit, Oil and Breaking Kit, and the Bead Recovery Reagents Kit according to the manufacturer’s instructions (Roche/454 Life Sciences, Branford, USA). Sequencing was carried out using the GS Junior Titanium Sequencing Kit and the GS Junior system (Roche/454 Life science, Branford, USA) following the manufacturer’s instructions without modifications.
Pithukpakorn M., Roothumnong E., Angkasekwinai N., Suktitipat B., Assawamakin A., Luangwedchakarn V., Umrod P., Thongnoppakhun W., Foongladda S, & Suputtamongkol Y. (2015). HLA-DRB1 and HLA-DQB1 Are Associated with Adult-Onset Immunodeficiency with Acquired Anti-Interferon-Gamma Autoantibodies. PLoS ONE, 10(5), e0128481.
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2

Pyrosequencing with GS Junior System

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Pyrosequencing was carried out according to the manufacturer's protocol for amplicons using the GS Junior System (Roche Diagnostics). Emulsion PCR, breaking, and bead enrichment was carried out using the GS Junior Titanium emPCR Kit Lib-L, emPCR Reagents Lib-L kit, Oil and Breaking kit, and the Bead Recovery Reagents kit according to the supplier's instructions (Roche Diagnostics). For emulsion PCR, we used a copy-per-bead ratio of 0.5. Enrichment of DNA-carrying beads was done with magnetic beads and a magnetic particle collector (Invitrogen, Carlsbad, CA, USA). To evaluate the amount of enriched beads, counting was performed using the GS Junior Bead Counter (Roche Diagnostics). Finally, we loaded 100,000-500,000 beads onto the PicoTiterPlate (Roche Diagnostics). Sequencing was carried out according to standard Roche/454 protocols using the GS Titanium Sequencing kit (Roche Diagnostics) and the GS Junior device.
Lee B., Lee B., Han G., Kwon M.J., Han J, & Choi Y.L. (2014). KRAS Mutation Detection in Non-small Cell Lung Cancer Using a Peptide Nucleic Acid-Mediated Polymerase Chain Reaction Clamping Method and Comparative Validation with Next-Generation Sequencing. Korean Journal of Pathology, 48(2), 100-107.
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3

Pyrosequencing of Amplicons using GS Junior

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Pyrosequencing of the amplicons was performed according to the manufacturer's protocol using the GS Junior System (Roche Diagnostics). Emulsion PCR, breaking, and bead enrichment were conducted using the GS Junior Titanium emPCR Kit, Lib-L emPCR Reagents, Lib-L Kit, Oil and Breaking Kit, and the Bead Recovery Reagents Kit according to the supplier's instructions (Roche Diagnostics). For emulsion PCR, we used a copy-per-bead ratio of 0.5. Enrichment of the DNA-carrying magnetic beads was accomplished using a magnetic particle collector (Invitrogen, Life Technologies). The quantity of the enriched beads was determined with the GS Junior Bead Counter (Roche Diagnostics). Finally, we loaded 100,000 to 500,000 beads onto the PicoTiterPlate (Roche Diagnostics). Sequencing was carried out according to standard Roche/454 protocols using the GS Titanium Sequencing Kit (Roche Diagnostics) and the GS Junior device.
Kang S., Kim B.G., Han H.H., Lee J.H., Kim J.E., Shim H.S, & Cho N.H. (2015). Targeted sequencing with enrichment PCR: a novel diagnostic method for the detection of EGFR mutations. Oncotarget, 6(15), 13742-13749.
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