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Digital imaging system

Manufactured by Sony
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Sourced in Japan

The Digital imaging system is a lab equipment product that captures and processes digital images. It is capable of converting optical images into digital data for storage, analysis, and further processing.

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Lab products found in correlation

Trc 50 9 camera, by Topcon (7 mentions) Fluorescent microscopy, by Nikon (1 mentions) Confocal microscopy, by Leica (1 mentions) Zonula occludens 1, by Thermo Fisher Scientific (1 mentions) Anti nlrp3, by Abcam (1 mentions) Vinculin, by Merck Group (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Caspase 1, by Thermo Fisher Scientific (1 mentions)

7 protocols using digital imaging system

1

Subretinal siRNA Delivery in Mice

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Subretinal injections of SINE RNAs in mice were performed as previously described (14 (link)). 1 μl of cholesterol-conjugated siRNA (2 μg/μl) targeting mouse Ddx17 or Luc were delivered as intravitreous injections three days prior to Alu RNA administration. The eye used for active versus control injection was chosen randomly. 7 days after SINE RNA injection, fundus photographs of dilated mouse eyes were captured by TRC-50 IX camera (Topcon) linked to a digital imaging system (Sony).
Wang S.B., Narendran S., Hirahara S., Varshney A., Pereira F., Apicella I., Ambati M., Ambati V.L., Yerramothu P., Ambati K., Nagasaka Y., Argyle D., Huang P., Baker K.L., Marion K.M., Gupta K., Liu B., Hinton D.R., Canna S.W., Sallam T., Sadda S.R., Kerur N., Gelfand B.D, & Ambati J. (2021). DDX17 is an essential mediator of sterile NLRC4 inflammasome activation by retrotransposon RNAs. Science immunology, 6(66), eabi4493.
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2

Fundus Imaging of Dilated Eyes

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Fundus imaging of dilated mouse and rat eyes was performed using a TRC-50 IX camera (Topcon) linked to a digital imaging system (Sony).
Fukuda S., Narendran S., Varshney A., Nagasaka Y., Wang S.B., Ambati K., Apicella I., Pereira F., Fowler B.J., Yasuma T., Hirahara S., Yasuma R., Huang P., Yerramothu P., Makin R.D., Wang M., Baker K.L., Marion K.M., Huang X., Baghdasaryan E., Ambati M., Ambati V.L., Banerjee D., Bonilha V.L., Tolstonog G.V., Held U., Ogura Y., Terasaki H., Oshika T., Bhattarai D., Kim K.B., Feldman S.H., Aguirre J.I., Hinton D.R., Kerur N., Sadda S.R., Schumann G.G., Gelfand B.D, & Ambati J. (2021). Alu complementary DNA is enriched in atrophic macular degeneration and triggers retinal pigmented epithelium toxicity via cytosolic innate immunity. Science Advances, 7(40), eabj3658.
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3

Fundus Imaging in Dilated Mouse Eyes

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A TRC-50 IX camera (Topcon) linked to a digital imaging system (Sony) was used for fundus photographs of dilated mouse eyes.
Kerur N., Fukuda S., Banerjee D., Kim Y., Fu D., Apicella I., Varshney A., Yasuma R., Fowler B.J., Baghdasaryan E., Marion K.M., Huang X., Yasuma T., Hirano Y., Serbulea V., Ambati M., Ambati V.L., Kajiwara Y., Ambati K., Bastos-Carvalho A., Ogura Y., Terasaki H., Oshika T., Kim K.B., Hinton D.R., Leitinger N., Cambier J.C., Buxbaum J.D., Kenney M.C., Jazwinski S.M., Nagai H., Hara I., West A.P., Fitzgerald K.A., Sadda S.R., Gelfand B.D, & Ambati J. (2017). cGAS drives non-canonical inflammasome activation in age-related macular degeneration. Nature medicine, 24(1), 50-61.
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4

Fundus Imaging in Dilated Mouse Eyes

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A TRC-50 IX camera (Topcon) linked to a digital imaging system (Sony) was used for fundus photographs of dilated mouse eyes.
Kerur N., Fukuda S., Banerjee D., Kim Y., Fu D., Apicella I., Varshney A., Yasuma R., Fowler B.J., Baghdasaryan E., Marion K.M., Huang X., Yasuma T., Hirano Y., Serbulea V., Ambati M., Ambati V.L., Kajiwara Y., Ambati K., Bastos-Carvalho A., Ogura Y., Terasaki H., Oshika T., Kim K.B., Hinton D.R., Leitinger N., Cambier J.C., Buxbaum J.D., Kenney M.C., Jazwinski S.M., Nagai H., Hara I., West A.P., Fitzgerald K.A., Sadda S.R., Gelfand B.D, & Ambati J. (2017). cGAS drives non-canonical inflammasome activation in age-related macular degeneration. Nature medicine, 24(1), 50-61.
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5

Retinal Imaging and Immunohistochemistry

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Subretinal injections and intravitreous injections (1 μL each) were performed with a 35-gauge Exmire microsyringe (Ito Corporation). Fundus imaging was performed on a TRC-50 IX camera (Topcon) linked to a digital imaging system (Sony). RPE flat mounts were immunolabeled using antibodies against Zonula Occludens-1 (Invitrogen)and visualized by confocal microscopy (Leica). Cryosections of 4% PFA fixed retinal cross sections were immuno-labeled with anti-Nlrp3 (Abcam) and visualized by fluorescent microscopy (Nikon).
Gelfand B.D., Wright C.B., Kim Y., Yasuma T., Yasuma R., Li S., Fowler B.J., Bastos-Carvalho A., Kerur N., Uittenbogaard A., Han Y.S., Lou D., Kleinman M.E., McDonald W.H., Núñez G., Georgel P., Dunaief J.L, & Ambati J. (2015). Iron toxicity in the retina requires Alu RNA and the NLRP3 inflammasome. Cell reports, 11(11), 1686-1693.
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6

Subretinal Injection Mouse Model for ZO-1 and Caspase-1 Analysis

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Fundus photographs of dilated mouse eyes were taken seven days after subretinal injection of ESAT-6, with TRC-50IX camera (Topcon, Tokyo, Japan) linked to a digital imaging system (Sony Corp., Tokyo, Japan). Following fundus photography, mice were sacrificed and whole mounts of RPE/ choroid were prepared that were fixed with 4% paraformaldehyde, and stained with rabbit polyclonal antibodies against mouse zonula occludens-1 (ZO-1, 1:100, Invitrogen, Carlsbad, CA), and visualized on immunofluorescence microscopy (SP-5, Leica, Exton, PA) with Alexa 594 (Invitrogen, Carlsbad, CA). Additional WT and Nlrp3 -/-mice were euthanized, and retina and RPE were collected separately in RIPA buffer with proteinase inhibitor, and homogenized by sonication. Protein abundance was tested by western blot analysis, with antibodies against caspase-1 (1:1000, Invitrogen) and vinculin (1:1000, Sigma-Aldrich, St. Louis, MO)
Basu S., Fowler B.J., Kerur N., Arnvig K.B, & Rao N.A. (2018). NLRP3 inflammasome activation by mycobacterial ESAT-6 and dsRNA in intraocular tuberculosis. Microbial pathogenesis, 114.
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7

Dilated Mouse Eye Fundus Imaging

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Fundus imaging of dilated mouse eyes was performed using a TRC-50 IX camera (Topcon) linked to a digital imaging system (Sony).
Narendran S., Pereira F., Yerramothu P., Apicella I., Wang S.B., Ambati K., Hirahara S., Kim Y., Ambati M., Ambati V.L., Huang P., Varshney A., Nagasaka Y., Fukuda S., Baker K.L., Marion K.M., Deussing J.M., Sadda S.R., Gelfand B.D, & Ambati J. (2021). Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration. Signal Transduction and Targeted Therapy, 6, 149.
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