The levels of cTnI, IL-1β and IL-18 in peripheral serum were measured with ELISAs (Elabscience, China) strictly following the instructions of the kit.
Elisas
Manufactured by Elabscience
Sourced in China
ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used analytical biochemistry assay that employs antibodies and color change to identify and quantify specific substances, typically proteins, peptides, hormones, or antibodies. The core function of an ELISA is to detect and measure the presence and/or concentration of a target analyte in a liquid sample.
Automatically generated - may contain errors
6 protocols using elisas
1
Measuring Serum Inflammatory Markers
2
Quantifying Membrane Proteins and Biomarkers
3
Venous Blood Sampling for Catecholamine Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood samples were collected from the jugular vein at baseline and 1 hour, and were centrifuged at 1000 g for 15 minutes. Supernatants were stored at −80°C for later analyses. ELISAs were used to measure the plasma levels of norepinephrine, according to the manufacturer's instruction (Elabscience).
Arterial blood for blood gas analysis was drawn from the femoral arterial sheath via an anaerobic heparinized syringe at baseline and 1.5 minutes after the last simulated OSA event. PaO2, PaCO2, and pH values were calculated using a VetScan i‐STAT1 Analyzer (Abbott Point of Care Inc) within 5 minutes of collection.
Arterial blood for blood gas analysis was drawn from the femoral arterial sheath via an anaerobic heparinized syringe at baseline and 1.5 minutes after the last simulated OSA event. PaO2, PaCO2, and pH values were calculated using a VetScan i‐STAT1 Analyzer (Abbott Point of Care Inc) within 5 minutes of collection.
4
Endothelial Glycocalyx and HMGB-1 Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Levels of circulating syndecan‐1, a component of the endothelial glycocalyx, and high mobility group protein B1 (HGMB‐1),26 damage marker released from predominantly platelets, were measured with ELISAs according to manufacturerʼs guidelines (eLabscience, USA).
5
Characterizing Inflammatory Cytokines in BAL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Additional details on the BAL processing can be found in Additional file 1 . Samples were analyzed using a FACSort flow cytometer. We also measured the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β using enzyme-linked immunosorbent assays (ELISAs) (Elabscience, China) according to the manufacturer's protocol.
6
Nestin and Cytokine Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA from the peritoneum samples collected from different week-old Nestin-GFP mice was extracted using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer's instructions. Real-time PCR for Nestin and β-actin mRNA expression was performed using the FastStart Universal SYBR Green Master (ROX) kit (Roche, Basel, Switzerland) with an ABI Prism 7900HT Sequence Detection System (Life Technology, Carlsbad, CA, USA) as previously described [21] .
The mRNA expression levels of Nestin were measured using β-actin as an internal reference gene. The primers included the following: mouse-Nestin forward 5¢-TCG CCA GGG AGG AGG CCA TT-3¢ and reverse 5¢-CTC CCC AGC CCT CCC CAG AC-3¢; mouse-β-actin forward 5¢-AGA GGG AAA TCG TGC GTG AC-3¢ and reverse 5¢-CAA TAG TGA TGA CCT GGC CGT-3¢. Relative expression was calculated using the 2 -ΔΔCT method.
Cytokine assays IL-33 concentrations were determined in cell culture supernatants and PDS by ELISAs following the manufacturer's instructions (Elabscience Biotechnology, Wuhan, China).
The mRNA expression levels of Nestin were measured using β-actin as an internal reference gene. The primers included the following: mouse-Nestin forward 5¢-TCG CCA GGG AGG AGG CCA TT-3¢ and reverse 5¢-CTC CCC AGC CCT CCC CAG AC-3¢; mouse-β-actin forward 5¢-AGA GGG AAA TCG TGC GTG AC-3¢ and reverse 5¢-CAA TAG TGA TGA CCT GGC CGT-3¢. Relative expression was calculated using the 2 -ΔΔCT method.
Cytokine assays IL-33 concentrations were determined in cell culture supernatants and PDS by ELISAs following the manufacturer's instructions (Elabscience Biotechnology, Wuhan, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!