Ms agilent 1100 series lc msd trap mass spectrometer

All chemicals (reagent grade) used were purchased from Sino Pharm Chemical Reagent Co., Ltd. (Shanghai, China). Reaction progress was monitored using analytical thin layer chromatography (TLC) on precoated silica gel GF254 (Qingdao Haiyang Chemical Plant, Qing-Dao, China) plates and the spots were detected under UV light (254 nm). Melting point was measured on an XT-4 micromelting point instrument and uncorrected. IR (KBr-disc) spectra were recorded by Bruker Tensor 27 spectrometer (Billerica, MA).
¹H NMR and 13C NMR spectra were measured on a BRUKER AVANCE III spectrometer (Billerica, MA) at 25 °C and referenced to TMS. Chemical shifts are reported in ppm (δ) using the residual solvent line as internal standard. Splitting patterns are designed as s, singlet; d, doublet; t, triplet; m, multiplet. The purity of all compounds was confirmed to be higher than 95% through analytical HPLC performed with Agilent 1200 HPLC System (Santa Clara, CA). Mass spectra were obtained on a MS Agilent 1100 Series LC/MSD Trap mass spectrometer (ESI-MS) (Santa Clara, CA).

All chemicals (reagent grade) used were purchased from Sino Pharm Chemical Reagent Co., Ltd. (Shanghai, China). Reaction progress was monitored using analytical thin layer chromatography (TLC) on pre-coated silica gel GF254 (Qingdao Haiyang Chemical Plant, Qingdao, China) plates, and spots were detected under UV light (254 nm). Melting point was measured on an XT-4 micromelting point instrument and uncorrected. IR (KBr-disc) spectra were recorded by a Bruker Tensor 27 spectrometer (Bruker, German). ¹H-NMR and ¹³C-NMR spectra were measured on a BRUKER AVANCE III spectrometer at 25 °C and referenced to tetramethyl silane (TMS) (Bruker, German). Mass spectra were obtained on an MS Agilent 1100 Series LC/MSD Trap mass spectrometer (Agilent, Santa Clara, CA, USA). UV-vis spectra were recorded on an Agilent 8454 UV-vis spectrometer (Agilent). Fluorescence spectra measurements were recorded on an Agilent G9800A fluorescence spectrophotometer (Agilent). Fluorescence images were obtained on a Leica TCS-SP8 multiphoton; a confocal microscope and a 63× oil-immersion objective lens was used (Leica, Switzerland). High-resolution EI mass spectra were recorded on an Agilent 6460 triple quad LC-MS mass spectrometer (Agilent).