Triisopropylsilane tips

Ultrapure water (Mili-Q, r > 18 MΩ·cm) was used in all the experiments. Acetonitrile (ACN), acetic acid (AcOH), dicholoromethane (DCM), diethyl ether (Et₂O), dimethylformamide (DMF), chloroform (CHCl₃), and methanol (MeOH) were obtained from Sigma-Aldrich (Saint-Quentin Fallavier, France) and were used without further purification.
The synthesis of c(RGDfC) peptide required N,N,N′,N′-tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU) (Iris Biotech GmbH, Germany) and acetic anhydride (Sigma-Aldrich, Saint-Quentin Fallavier, France), and N–methylmorpholine (NMM), N–methylpyrrolidinone (NMP), N,N-diisopropylethylamine (DIPEA), piperidine and tetrakis(triphenylphosphine)palladium(0) (Pd(PPh₃)₄) (Alfa Aesar, Haverhill, MA, USA) as reagents. Fmoc–L–Asp–OAll, Fmoc–Gly–OH, Fmoc–L–Arg(Pbf)–OH, Fmoc–L–Cys (Trt)–OH, Fmoc–D–Phe–OH and 2CTC resin (2-chlorotrityl chloride resin, 100–200 mesh, 1.0–2.0 mmol/g loading) were obtained from Iris Biotech GmbH (Marktredwitz, Germany). Trifluoroacetic acid (TFA) was purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France) and triisopropylsilane (TIPS) from Alfa Aesar (Haverhill, MA, USA).
The synthesis of DSPE-PEG₅₀₀₀-c(RGDfC) required DSPE-PEG₅₀₀₀-Mal (≥95%, Biochempeg Scientific Inc., Watertown, MA, USA), and phosphate-buffered saline (pH 7.4) and 1 kDa dialysis membrane (Sigma-Aldrich, Saint-Quentin Fallavier, France).

𝛽-LG, tris (hydroxymethyl) aminomethane (Tris) hydrochloride, TC, QC, BR, CUR, resazurin, agar, methanol (MeOH), ethanol (EtOH), sodium dodecyl sulfate (SDS), hydrochloric acid (HCl), Mueller—Hinton broth (MHB), and dimethylsulfoxide (DMSO) were purchased from Merck (Castle Hill, NSW, Australia). Propylene glycol liquid (USP) was purchased from the Melbourne Food Ingredient Depot (Melbourne, VIC, Australia). Triton X-100, CaCl₂ and NaCl were purchased from Chem-Supply (Gillman, SA, Australia). Triisopropylsilane (TIPS) was purchased from Alfa Aesar (Tewksbury, MA, USA). The SrtA_ΔN59 enzyme was expressed and purified as previously described [4 (link)]. Dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Thermo Fisher Scientific (Scoresby, VIC, Australia). PEX, INDO, MASTO, the quenched fluorescent resonance energy transfer (FRET) peptide (Abz-LPETGK(Dnp)-NH₂), and Gly₃-amide peptides were synthesized as previously described [9 (link)]. The bacterial library used herein consisted of S. aureus (ATCC25923), MRSA (ATCC43300), E. coli (ATCC25922), and P. aeruginosa (ATCC27853).

The bacterial strains (methicillin-sensitive S. aureus (MSSA) ATCC 25923, methicillin-resistant Staphylococcus aureus (MRSA) ATCC43300, E. coli ATCC 25922, and P. aeruginosa ATCC 27853) were obtained from the American Type Culture Collection (Manassas, VA, USA). All amino acids and Rink amide-4-methylbenzhydrylamine (MBHA) resin (0.54 mmol/g) were obtained from Mimotopes (Melbourne, VIC, Australia). Ethyl (hydroxyimino)cyanoacetate (Oxyma Pure) and N,N′-diisopropylcarbodiimide (DIC) were obtained from Chem-Impex International (Wood Dale, IL, USA). Triisopropylsilane (TIPS) was obtained from Alfa Aesar (Massachusetts, VA, USA). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were obtained from Thermo Fisher Scientific (Scoresby, VIC, Australia). Acetonitrile (MeCN), cetyltrimethylammonium bromide (CTAB, 99%), N,N-dimethylformamide (DMF; peptide synthesis grade), dimethyl sulfoxide (DMSO), ethanol (EtOH), methanol (MeOH), Mueller–Hinton broth (MHB), tetraethyl orthosilicate (TEOS, reagent grade 98%), trifluoroacetic acid (TFA), 3-(trihydroxysilyl)propyl methylphosphonate (THMP, 90%), and all other reagents were purchased from Merck (Castle Hill, NSW, Australia).