Ab51317

Manufactured by Abcam

Sourced in United States

Ab51317 is a lab equipment product offered by Abcam. It serves as a core laboratory tool, but a detailed description of its function cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using ab51317

Immunofluorescent Analysis of Mouse Lung Tissue

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

We followed the methods of Sun et al. [17 (link)]. Four percent paraformaldehyde-fixed mouse lung tissues were cut into 4 μm thick sections. After dehydration, the sections were collected on Superfrost Plus glass slides. Sections were rinsed with PBS and permeabilized with a 1% Triton solution for 5 min. The cells were then blocked with 1% BSA for 1 h. Primary antibodies against α-SMA (Abcam, Ab240654, 1:200), SENP1 (Abcam, Ab236094, 1:200), and Sca-1 (Abcam, Ab51317, 1:200) were incubated with the sections overnight at 4 °C. Secondary antibodies were incubated with the samples for 1 h at room temperature. The sections were mounted with Fluorescent Mounting Media with DAPI and observed under a Leica SP8 confocal laser scanning microscope at magnifications of × 200.

Sun W., Liu X., Yang X., Jing X., Duan C., Yang G., Wu C., Huang H., Luo Q., Xia S., Zhang Q., Yang Y, & Xu Z. (2022). SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression. Cell Communication and Signaling : CCS, 20, 104.

+ Open protocol

+ Expand

Multiparametric Immunofluorescence Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies were directed to Ki-67 (AB16667, Abcam), calponin (SC136987, Santa Cruz), BECN1 (sc-11427, Santa Cruz), LC3B (#3868, Cell Signaling), LAMP1 (sc-17768, Santa Cruz), F4/80 (ab6640, Abcam), CD11b (PA5-18727, Invitrogen), Sca1 (ab-51317, Abcam), Osteopontin (ab8488, Abcam), LC3A/B (ab58610, Abcam). Secondary antibodies for immunofluorescence were Alexa Green 488 nm and Alexa Orange-Red 546 nm (Invitrogen). 4,6-diamidino-2-phenylindole (DAPI) (D3571), Prolong Gold Anti-fade mounting reagent (P36930), Slow-Fade Anti-fade reagent (S2828). Osteopontin was obtained from SIGMA (Cat. # O2260).

Pestell T.G., Jiao X., Kumar M., Peck A.R., Prisco M., Deng S., Li Z., Ertel A., Casimiro M.C., Ju X., Di Rocco A., Di Sante G., Katiyar S., Shupp A., Lisanti M.P., Jain P., Wu K., Rui H., Hooper D.C., Yu Z., Goldman A.R., Speicher D.W., Laury-Kleintop L, & Pestell R.G. (2017). Stromal cyclin D1 promotes heterotypic immune signaling and breast cancer growth. Oncotarget, 8(47), 81754-81775.

+ Open protocol

+ Expand

Comprehensive Antibody Panel for Vascular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For Western blotting and immunostainings, the following antibodies were used: VE‐CADHERIN rat (550548, BD Biosciences); VE‐CADHERIN goat (sc‐6458, Santa Cruz); PECAM1 hamster (MAB1398Z, Millipore); PECAM1 rat (553370, BD); PECAM1 rabbit (ab28364, Abcam); mKLF4 goat (AF3158, R&D); hKLF4 goat (AF3640, R&D); FSP1 rabbit (07‐2274, Millipore); ID1 rabbit (BCH‐1/37‐2, BIOCHECK); SCA1 rat (ab51317, Abcam); hCLAUDIN5 rabbit (ab53765, Abcam); pSMAD1/5 rabbit (9516, Cell Signaling); SMAD1 rabbit (9644, Cell Signaling); Glucose transporter type 1 (GLUT1) rabbit (RB9052P1, Thermo Scientific); ERK5 rabbit (07‐039, Upstate); VE‐PTP rabbit (produced and purified by New England Peptide); MEKK3 rabbit (5727, Cell Signaling); MEK5 mouse (610957, BD); BMP6 sheep (LS‐C150156, LS‐BIO); GAPDH mouse (SC‐32233, Santa Cruz); tubulin mouse (T9026, Sigma); vinculin mouse (V9264, Sigma); horseradish peroxidase (HRP)‐linked anti‐mouse, anti‐rat, and anti‐rabbit (Cell Signaling); and HRP‐linked anti‐goat (Promega). Biotin‐conjugated isolectin B4 (Vector Lab) was used to identify retinal vasculature. ALEXA FLUOR 488, 555, and 647 donkey secondary antibodies were from Life Technologies.

Cuttano R., Rudini N., Bravi L., Corada M., Giampietro C., Papa E., Morini M.F., Maddaluno L., Baeyens N., Adams R.H., Jain M.K., Owens G.K., Schwartz M., Lampugnani M.G, & Dejana E. (2015). KLF4 is a key determinant in the development and progression of cerebral cavernous malformations. EMBO Molecular Medicine, 8(1), 6-24.

+ Open protocol

+ Expand

Protein Expression Analysis of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell culture media and sera were purchased from Thermo Fisher Scientific, whereas cell culture supplements, growth factors, and inhibitors were purchased from Sigma. The XBP1u and XBP1s antibodies were raised by GenScript using peptide CRSSQRTQKDPVPY (XBP1u) and DSGGIDSSDSESDIC (XBP1s). The antibodies against Sca-1 (ab51317), IRE1α (ab37073), COL4A1 (ab6586), Col15A1 (ab58717), and Col18A1 (ab52022) were purchased from Abcam; antibody for GAPDH (sc-25778) was from Santa Cruz Biotechnology; antibody for FLAG (A4596 and F1804), alpha SMA (A5228), and all other chemicals from Sigma; all secondary antibodies were purchased from Dakocytomation.

Angbohang A., Huang L., Li Y., Zhao Y., Gong Y., Fu Y., Mao C., Morales J., Luo P., Ehteramyan M., Gao Y., Margariti A., Gu W., Zhang M., Smith A., Shah A.M., Li T., Kong W, & Zeng L. (2021). X-box binding protein 1–mediated COL4A1s secretion regulates communication between vascular smooth muscle and stem/progenitor cells. The Journal of Biological Chemistry, 296, 100541.

+ Open protocol

+ Expand

Profiling SMC and Stem Cell Markers by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The SMC differentiation markers and stem cell markers using flow cytometry. The cells were cultured in a Fluorescence-Activated Cell Sorting (FACS) buffer containing 2% FBS and PBS. After fixation and permeabilization, SMC-specific markers myosin heavy chain 11 (Myh11; ab224805, 1:80; Abcam), calponin 1 (ab46794, 1:100; Abcam), Smoothelin2 (ab236034, 1:50; Abcam) and stem cell markers CD44 (ab238464, 0.474 μg/ml; Abcam), stem cell antigen-1 (Sca1; ab51317, 0.2 μg/10⁶ cells; Abcam) and S10 calcium-binding protein B (S100β; Thermo Fisher Scientific, Waltham, MA, USA) were added. All antibodies were used at the manufacturer’s recommended dilutions and cellular concentrations. Subsequently, the signals of labelled cells were detected by flow cytometry and the data were analyzed by FlowJo software (Beckman Coulter, Fullerton, CA, USA).

Zhang Z., Mu X, & Zhou X. (2022). Dexmedetomidine alleviates inflammatory response and oxidative stress injury of vascular smooth muscle cell via α2AR/GSK-3β/MKP-1/NRF2 axis in intracranial aneurysm. BMC Pharmacology & Toxicology, 23, 81.

+ Open protocol

+ Expand

Surface Marker Expression Analysis of Cell Spheroids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The expression levels of the CSps surface markers CD31 (1:200, GB11063-3, Servicebio), CD34 (1:200, ab81289, Abcam), CD90 (1:200, ab225, Abcam), CD105 (1:200, ab156756, Abcam), Sca-1 (1:200, ab51317, Abcam), and KDR (1:200, sc6251, Santa Cruz) were determined by flow cytometry. After a 3-day cultivation, cells and CSps from different substrates were obtained and digested into single cells. After incubation with the primary antibodies for 1 h and the corresponding secondary antibodies for 30 min, Alexa Fluor 488 goat anti-mouse IgG (1:200, ab150113, Abcam) or Alexa Fluor 488 goat anti-rabbit IgG (1:200, ab150077, Abcam) was used. The staining results were analyzed by flow cytometry (BD FACSCanto), and a negative isotypic control was used during the analysis.

Wang Y., Li Q., Zhao J., Chen J., Wu D., Zheng Y., Wu J., Liu J., Lu J., Zhang J, & Wu Z. (2023). Mechanically induced pyroptosis enhances cardiosphere oxidative stress resistance and metabolism for myocardial infarction therapy. Nature Communications, 14, 6148.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!