[18F]DPA-714 was prepared in a GE TRACERlab FX-FN synthesizer in accordance with a published procedure [22 (link)]. In brief, after the one-step synthesis consisting of a nucleophilic substitution of the precursor N,N-diethyl-2-(2-(4-(2-toluenesulfonyloxyethoxy)-phenyl)5,7dimethylpyrazolo[1,5a] pyrimidin-3-yl) acetamid with [18F]fluoride, the crude product mixture was purified by high-performance liquid chromatography (λ, 254 nm; flow, 3.0 mL/min; column, ACE 126–2510, 10 mm × 250 mm; eluent, 0.1 M NH4OAc/EtOH 6/4 (v/v) pH 10.0).The resulting product batch was subjected to a sterile filtration using a 0.2-μm filter (Sterifix® Paed, B. Braun) and dispensed automatically in a closed vial filling in 10-mL vials. The batch was released for human application after quality control (QC) including the determination of the pH value (4.0–8.5), the osmolality (< 3000 mOsm/kg), the radiochemical purity (≥ 95%), the filter integrity (“bubble-point” test), endotoxins (≤ 17.5 EU/mL), and the content of residual solvents and chemical impurities (DMSO, acetonitrile, EtOH, kryptofix® 222).
Tracerlab fx fn synthesizer
Manufactured by GE Healthcare
Sourced in United States
The TRACERlab FX-FN synthesizer is a compact, automated radiopharmaceutical synthesis module designed for the production of fluorine-18 (F-18) labeled compounds. The device provides a controlled environment for the synthesis, purification, and formulation of F-18 tracers, ensuring consistent and reliable production.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using tracerlab fx fn synthesizer
1
Automated Radiosynthesis of [18F]DPA-714
2
Radiosynthesis of [18F]DPA-714 and [18F]FDG
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
[18F]FDG was purchased from the commercially available source Cyclopharma S.A. (Clermont-Limagne, France). [18F]DPA-714 was produced on site according to slight modifications of procedures already reported [20 ] and using a commercially available GE TRACERLab FX-FN synthesizer [21 (link)]. Ready-to-inject, >99 % radiochemical pure [18F]DPA-714 (formulated in physiological saline containing less than 10 % of ethanol) was obtained with 15–20 % non-decay-corrected yields and specific radioactivitiy level at the end of the radiosynthesis ranging from 37 to 111 GBq/μmol.
3
PET-CT Imaging of Brain Glucose Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 6-h fasting for reducing glucose uptake, patients underwent PET-CT examination and intravenous injection of 18F-FDG. Brain imaging was performed 30 min later, and the metabolic process and distribution situation of 18F-FDG were monitored in brain tissue; meanwhile, a CT Tomoscan scan was conducted for reconstructed images. Appearance of continuous high or low metabolic zones in two levels was considered as abnormal, and when the relative intensity of radioactivity was reduced or increased by 15% in bilateral corresponding zones, this was regarded as indicating a metabolic abnormality. The localization diagnosis for epileptic foci was performed in terms of brain function and imageology. The Discovery 16 CT and MINItrace Cyclotron produced by GE Corp. (USA) were used. 18F-FDG PET-CT was performed using a Tracerlab FXFN synthesizer from GE Medical Systems (USA), with a radiochemical purity of FDG >95%. According to scan plan, multilayer CT scanning was initially conducted with parameters as follows: voltage, 120 kV; electric current, 180 mA; 3D model reconstruction, FORE-interactive reconstruction; axial field-of-view (FOV), 25 cm; emission scanning acquisition, 25 min; thickness, 3.75 mm; matrix, 128×128; scanning range, entire head. A total of 40 cross-sectional images were obtained, and image fusion was performed through an Xeleris and AW4.3 workstation.
4
Synthesis and Radiolabeling of Fluspidine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The synthesis of (S)-(−)-[18F]fluspidine for the human application was performed as described by Fischer et al. [8 (link)] with minor modifications. Briefly, the tracer was produced by phase transfer catalyst assisted nucleophilic substitution (100 °C, 15 min) using a precursor molecule with a tosyl-leaving group (2 mg in 1 mL dry CH3CN). Purification and formulation was achieved by semipreparative HPLC and solid phase extraction, respectively. Overall synthesis time was 50 min, radiochemical purity exceeded 97% and specific activity was determined to be 230 ± 160 GBq/μmol (n = 16 syntheses).
For the animal studies, enantiomerically pure (S)-(−)-[18F]fluspidine and (R)-(+)-[18F]fluspidine was prepared on a TRACERlab FX F-N synthesizer (GE Healthcare) as described in previous publications [9 (link),47 (link)]. The radiochemical purity of (R)-(+) or (S)-(−)-[18F]fluspidine was >99%, and the specific activity at the end of the synthesis was 650 and 870 GBq/μmol, respectively [48 ].
For the animal studies, enantiomerically pure (S)-(−)-[18F]fluspidine and (R)-(+)-[18F]fluspidine was prepared on a TRACERlab FX F-N synthesizer (GE Healthcare) as described in previous publications [9 (link),47 (link)]. The radiochemical purity of (R)-(+) or (S)-(−)-[18F]fluspidine was >99%, and the specific activity at the end of the synthesis was 650 and 870 GBq/μmol, respectively [48 ].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!