Lc q tof ms 6520 spectrometer

All reagents from commercial suppliers were used without further purification. All solvents were freshly distilled before use from appropriate drying agents. All other reagents were recrystallized or distilled when necessary. Analytical TLCs were performed with silica gel 60 F254 plates. Visualization was accomplished by UV light. Column chromatography was carried out using silica gel 60 (230–400 mesh ASTM). NMR spectra were obtained on a Bruker Avance 500 MHz spectrometers and recorded at 25°C. Chemical shifts for ¹H-NMR spectra are reported in ppm downfield from TMS, chemical shifts for ¹³C-NMR spectra are recorded in ppm relative to internal deuterated chloroform (δ = 77.2 ppm for ¹³C). Coupling constants (J) are reported in Hertz. The terms m, s, d, t, q refers to multiplet, singlet, doublet, triplet, quartet. ¹³C-NMR were broadband decoupled from hydrogen nuclei. High resolution mass spectra (HRMS) was measured by EI method with a Agilent LC-Q-TOF-MS 6520 spectrometer.

All reagents from commercial suppliers were used without further purification. All solvents were freshly distilled before use from appropriate drying agents. All other reagents were recrystallized or distilled when necessary. Reactions were performed under a dry nitrogen atmosphere. Analytical TLCs were performed with silica gel 60 F₂₅₄ plates. Visualization was accomplished by UV light. Column chromatography was carried out using silica gel 60 (230–400 mesh ASTM). Melting points were determined with an Electrothermal IA9100 Digital Melting Point Apparatus without correction. NMR spectra were obtained on a Bruker Avance 400 MHz and a Varian VXR 300 MHz spectrometer and recorded at 25 °C. Chemical shifts for ¹H NMR spectra are reported in ppm downfield from TMS, chemical shifts for ¹³C NMR spectra are recorded in ppm relative to internal deuterated chloroform (δ = 77.2 ppm for ¹³C), chemical shifts for ¹⁹F NMR are reported in ppm downfield from fluorotrichloromethane (CFCl₃). Coupling constants (J) are reported in Hertz. The terms m, s, d, t, q refer to multiplet, singlet, doublet, triplet, quartet. ¹³C NMR and ¹⁹F NMR were broadband decoupled from hydrogen nuclei. High-resolution mass spectra (HRMS) were measured by the EI method with an Agilent LC-Q-TOF-MS 6520 spectrometer.

All reagents from commercial suppliers were used without further purification. All solvents were freshly distilled before use from appropriate drying agents. All other reagents were recrystallized or distilled when necessary. Reactions were performed under a dry nitrogen atmosphere. Analytical thin layer chromatography (TLC) was performed with silica gel 60 F₂₅₄ plates. Visualization was accomplished by UV light. Column chromatography was carried out using silica gel 60 (230–400 mesh ASTM). Melting points were determined with an Electrothermal IA9100 Digital Melting Point Apparatus without correction. NMR spectra were obtained on Bruker Avance 400 MHz and Varian VXR 300 MHz spectrometers and recorded at 25 °C. Chemical shifts for ¹H NMR spectra are reported in ppm downfield from TMS, chemical shifts for ¹³C NMR spectra are recorded in ppm relative to internal deuterated chloroform (δ = 77.2 ppm for ¹³C), and chemical shifts for ¹⁹F NMR are reported in ppm downfield from fluorotrichloromethane (CFCl₃). Coupling constants (J) are reported in Hertz. The terms m, s, d, t, and q refer to multiplet, singlet, doublet, triplet, and quartet, respectively. ¹³C NMR and ¹⁹F NMR were broadband decoupled from hydrogen nuclei. High-resolution mass spectra (HRMS) were measured by EI method with an Agilent LC-Q-TOF-MS 6520 spectrometer.