Endothelial colony-forming cells (ECFCs) were isolated from the adherent mononuclear cell fraction of human peripheral blood using CD31-coated magnetic beads (Invitrogen, MA, USA) as previously described [11 (link)]. Isolated ECFCs were expanded on 1% gelatin-coated plates (BD Biosciences, NJ, USA) using an endothelial cell growth medium MV 2 (EGM-MV 2 without hydrocortisone; PromoCell, Heidelberg, Germany) supplemented with 10% fetal bovine serum (Atlas Biologicals, CO, USA) and 1% glutamine-penicillin-streptomycin (Gibco, MA, USA). ECFCs between passages seven and ten were used in all of the experiments. The protocol for this section of the study was approved by the institutional review board of Duksung Women's University (IRB Nos. 2017-002-001 and 2018-007-006).
Egm mv 2 without hydrocortisone
Manufactured by PromoCell
Sourced in Germany
EGM-MV 2 without hydrocortisone is a basal medium for the culture of human microvascular endothelial cells. It is formulated to support the growth and proliferation of these cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Cd31 coated magnetic beads, by Thermo Fisher Scientific (2 mentions)
Glutamine penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlas Biologicals (1 mentions)
Glutamine penicillin streptomycin gps, by Thermo Fisher Scientific (1 mentions)
Gelatin coated plates, by BD (1 mentions)
2 protocols using egm mv 2 without hydrocortisone
1
Isolation and Expansion of Human ECFCs
2
Isolation and Expansion of Endothelial Colony-Forming Cells
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The study protocol was approved by the Institutional Review Board (IRB) of Duksung Women's University (IRB No. 2017-002-001, 2018-007-006). ECFCs were isolated from the adherent mononuclear cell fraction of human peripheral blood using CD31-coated magnetic beads (Invitrogen, Waltham, MA, USA) as described previously.14 (link) Isolated ECFCs were expanded on 1% gelatin-coated plates (BD Biosciences, Franklin Lakes, NJ, USA) using EC growth medium MV 2 (EGM-MV 2 without hydrocortisone; Promocell, Heidelberg, Germany) supplemented with 10% fetal bovine serum (FBS; Atlas Biologicals, Fort Collins, CO, USA) and 1% glutamine-penicillin-streptomycin (GPS; Gibco, Waltham, MA, USA). ECFCs obtained between passages 7 and 10 were used for all experiments.
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