Tia 1

Manufactured by Beckman Coulter

Sourced in United Kingdom

The TIA‐1 is a laboratory instrument designed for the detection and quantification of specific proteins or analytes in a sample. It utilizes a technology called Time-Resolved Immunoassay (TIA) to perform these measurements. The core function of the TIA‐1 is to provide accurate and reliable analytical results for research and diagnostic applications.

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Lab products found in correlation

Granzyme b, by Agilent Technologies (1 mentions) T bet, by Abcam (1 mentions) Gata3, by Cell Signaling Technology (1 mentions) Mib 1, by Agilent Technologies (1 mentions) Granzyme b, by Leica (1 mentions) Betaf1, by Thermo Fisher Scientific (1 mentions)

2 protocols using tia 1

Immunohistochemical Evaluation of T-cell Markers

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The antibodies used for IHC were CD3 (M7254, DAKO), CD4 (790–4423, VENTANA), CD8 (M7103, DAKO), CD30 (M0751, DAKO), TIA‐1 (IM2550, BECKMAN COULTER), Granzyme B (M7235, DAKO), T‐bet (4B10, Abcam), CXCR3 (1C6, BD), GATA3 (5852, Cell signaling Technology), and CCR4 (1G1, BD). Except for T‐bet, CXCR3, GATA3, and CCR4, the cutoff value for positivity was 30%. If neoplastic cells were positive for TIA‐1 and/or Granzyme B, the patient was considered positive for cytotoxic molecules.

Shimasaki Y., Miyoshi H., Kawamoto K., Yoshida N., Mishina T., Nakashima K., Imamoto T., Sugio T., Yanagida E., Kato T., Yamada K., Takeuchi M., Suzuki T., Moritsubo M., Furuta T., Imaizumi Y., Takizawa J., Kato K., Suzumiya J., Suzuki R, & Ohshima K. (2024). Clinicopathological comparison between PTCL‐TBX21 and PTCL‐GATA3 in Japanese patients. Cancer Medicine, 13(3), e6793.

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Comprehensive Immunohistochemical Profiling of Skin Biopsies

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FFPE sections of skin biopsies were histologically examined in order to evaluate the pattern and composition of the infiltrate.
The large panel of immunohistochemistry tests included CD3, CD4 (Dako, Glostrup, Denmark), CD5 (Novocastra, Newcastle upon Tyne, UK), CD7, CD8, CD30, CD45RO, CD45RA (Dako), CD56 (Novocastra), CCR4 (Abcam, Cambridge, UK), TIA-1 (Beckman Coulter, Indianapolis, USA), granzyme B, perforin (Novocastra), BetaF1 (Thermo Fisher, Waltham, USA), TCR Vδ1, TCR Vδ2 (Beckman Coulter), TCRγ (Thermo Fisher), and MIB-1 (Dako).

Alberti-Violetti S., Maronese C.A., Venegoni L., Merlo V, & Berti E. (2021). Primary Cutaneous Gamma-Delta T Cell Lymphomas: A Case Series and Overview of the Literature. Dermatopathology, 8(4), 515-524.

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