Cc 2545

Manufactured by Lonza

CC-2545 is a laboratory instrument designed for cell culture applications. It provides a controlled environment for maintaining and growing cell cultures. The core function of this product is to regulate temperature, humidity, and gas composition within the chamber to support optimal cell growth conditions.

Automatically generated - may contain errors

Lab products found in correlation

C 12281, by PromoCell (1 mentions) Cc 3162, by Lonza (1 mentions) Axiovert 200m inverted microscope, by Zeiss (1 mentions) C 12212, by PromoCell (1 mentions) Pcs 100 011, by American Type Culture Collection (1 mentions) C 12203, by PromoCell (1 mentions) Fetal bovine serum (fbs), by Omega Scientific (1 mentions) Cc 2583, by Lonza (1 mentions)

2 protocols using cc 2545

Endothelial Cell Culture and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human ECs were purchased from different vendors to capture a broad spectrum of sampling. Specifically, we obtained HUVECs (C2519A; Lonza; C-12203; PromoCell), HSaVECs (HSVEC/A; VEC-Technologies; cAP-0019; Angio-Proteomie), HAECs (PCS-100-011; ATCC; 6100; ScienCell), HIAECs (CC-2545; Lonza; cAP-0020; Angio-Proteomie), HBMVECs (ACBRI 376 V; Cell-Systems; cAP-0002; Angio-Proteomie), HUMVECs (C-12295; PromoCell; 7000; ScienCell), HLMVECs (3000; ScienCell; C-12281; PromoCell), HAMVECs (7200; ScienCell), and HDMECs (C-12212; PromoCell; 2000; ScienCell). All types of ECs were cultured in EGM-2 endothelial growth medium (CC-3162; Lonza) supplemented with 5% FBS (Omega Scientific) and were maintained at 37°C with 5% CO₂. Similar EC passages 4–6 were used for final experiments. Human dermal fibroblasts were a gift from W. Lowry (University of California, Los Angeles, Los Angeles, CA), expanded in DMEM supplemented with 10% FBS, and cultured in EMG-2 medium over 48 h before RNA expression experiments. Brightfield images were acquired by using an Axiovert 200M inverted microscope (Zeiss).

Hilfenhaus G., Nguyen D.P., Freshman J., Prajapati D., Ma F., Song D., Ziyad S., Cuadrado M., Pellegrini M., Bustelo X.R, & Iruela-Arispe M.L. (2018). Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers. The Journal of Cell Biology, 217(8), 2813-2830.

+ Open protocol

+ Expand

Isolation and Characterization of Human Coronary Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human primary coronary artery endothelial cells (CAEC) and CAVSMCs from healthy individuals were purchased from Lonza (CC-2545 and CC-2583). Human primary coronary artery vascular smooth muscle cells from T2DM patients with CVD were isolated as previously reported by Dr. Pereira (Dinardo et al., 2015 ; Dinardo et al., 2014 (link); Vaquero et al., 2012 (link)).

Toyohara T., Roudnicky F., Florido M.H., Nakano T., Yu H., Katsuki S., Lee M.J., Torsten M., Friesen M., Davidow L.S., Ptaszek L., Abe T., Rubin L.L., Pereira A.C., Aikawa M, & Cowan C.A. (2020). Patient hiPSCs identify vascular smooth muscle arylacetamide deacetylase as protective against atherosclerosis. Cell stem cell, 27(1), 147-157.e7.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!