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L lactate assay kit 1

Manufactured by Eton Bioscience
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The L-Lactate Assay Kit is a colorimetric assay that quantifies the concentration of L-lactate in biological samples. The kit uses an enzymatic reaction to produce a colored product, the intensity of which is proportional to the L-lactate concentration.

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Lab products found in correlation

Glucose assay kit, by Eton Bioscience (2 mentions) Iron sl assay, by Sekisui (2 mentions) Glucose assay kit 1, by Eton Bioscience (2 mentions) Picoprobe acetyl coa fluorometric assay kit, by Abcam (1 mentions) Glucose hk assay kit, by Merck Group (1 mentions) 2 nbdg, by Abcam (1 mentions) Azd3965, by MedChemExpress (1 mentions) 2 deoxy d glucose, by Merck Group (1 mentions) Sodium l lactate, by Merck Group (1 mentions) Dimethyl 2 oxoglutarate, by Merck Group (1 mentions) L type tg m assay, by Fujifilm (1 mentions) Erastin, by Merck Group (1 mentions) Celltracker green, by Thermo Fisher Scientific (1 mentions) Nocodazole ncz, by Merck Group (1 mentions) Microtubules tubulin in vivo assay kit, by Cytoskeleton (1 mentions) Paclitaxel ptx, by Merck Group (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by American Type Culture Collection (1 mentions) Hyaluronan duoset elisa, by R&D Systems (1 mentions) 660 nm protein assay kit, by Thermo Fisher Scientific (1 mentions)

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Lactate Assay Kit I (2 citations)

19 protocols using l lactate assay kit 1

1

Lactate Production Quantification Assay

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Lactate production was analyzed in media from cells treated with vehicle control or everolimus using the L-Lactate Assay Kit I (Eton Bioscience, San Diego, California) per manufacturer’s instructions. Conditioned media were collected by centrifuging cells at 2000g and removing supernatant. Media were immediately frozen at −80°C and thawed immediately prior to analysis. A standard curve was generated using lactate and bovine serum albumin standards provided in the respective kits. The lactate concentration of samples was determined by plotting results on the standard curve, normalized to protein concentration and accounting for the lactate concentration of media.
Akhenblit P.J., Hanke N.T., Gill A., Persky D.O., Howison C.M., Pagel M.D, & Baker A.F. (2016). Assessing Metabolic Changes in Response to mTOR Inhibition in a Mantle Cell Lymphoma Xenograft Model Using AcidoCEST MRI. Molecular imaging, 15, 1536012116645439.
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2

Plasma EPO and Lactic Acid Assay

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Whole blood (100 μl) collection was performed as described as above. The plasma was separated by centrifugation at 4,000 rpm for 15 min and stored at −80 °C until assayed. EPO levels were measured using a Mouse Erythropoietin/EPO ELISA Kit (LifeSpan BioSciences) according to the manufacturer’s instructions. Lactic acid was assayed using a L-lactate Assay Kit I (Eton Bioscience) according to the manufacturer’s instructions.
Luo S.T., Zhang D.M., Qin Q., Lu L., Luo M., Guo F.C., Shi H.S., Jiang L., Shao B., Li M., Yang H.S, & Wei Y.Q. (2017). The Promotion of Erythropoiesis via the Regulation of Reactive Oxygen Species by Lactic Acid. Scientific Reports, 7, 38105.
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3

Metabolite Detection in Cell Culture

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The concentration of glucose and lactate in the cell culture medium and of PEP and acetyl-CoA in cell lysate was detected by a glucose assay kit (Eton Bioscience), L-lactate assay kit I (Eton Bioscience), PEP Fluorometric Assay Kit (Cayman) and the PicoProbe™ Acetyl-CoA Fluorometric Assay Kit (Biovision), respectively. The assays were performed as instructed by the manufacturer.
Zhao J., Li J., Fan T.W, & Hou S.X. (2017). Glycolytic reprogramming through PCK2 regulates tumor initiation of prostate cancer cells. Oncotarget, 8(48), 83602-83618.
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4

Glucose and Lactate Quantification in Osteogenic DDCs

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DDCs transduced with LV-NS or LV-181a/b-1 were seeded in 6-well plates (1 × 106 cells/well) containing 1ml phenol red-free osteogenic induction medium. After 24 h in culture, the medium was collected and the glucose concentration determined using the Glucose (HK) Assay Kit (Sigma, GAHK-20) following the manufacturer’s protocol. Lactate concentration in the culture medium was measured using the L-Lactate Assay Kit I (Eton Bioscience Inc; #1200011002) following the manufacturer’s recommendation.
Zheng H., Liu J., Tycksen E., Nunley R, & McAlinden A. (2019). MicroRNA-181a/b-1 over-expression enhances osteogenesis by modulating PTEN/PI3K/AKT signaling and mitochondrial metabolism. Bone, 123, 92-102.
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5

Metabolic Regulation of Peritoneal Macrophages

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Peritoneal macrophages were stimulated with MLE-12 or LLC Exo. (40μg/mL) for 16 hours. Cells were then washed and incubated in glucose free RPMI for 30 minutes before 2-NBDG (400μM, BioVision) was added. Cells were incubated with 2-NBDG for 15 minutes before washing and staining with viability dye and F4/80 for analysis via flow cytometry. The following concentrations of metabolic inhibitors or substrates were used for 16-hour cell culture: 2-deoxy-D-glucose (1mM, Sigma), SEITU (2–4mM, Cayman Chemical), Dimethyl-2-oxoglutarate (1mM-5mM, Sigma), Sodium-L-Lactate (20mM, Sigma), AZD3965 (250nM, MedChemExpress). Levels of L-lactate in the supernatants were measured by L-Lactate Assay Kit I (Eton Bioscience, San Diego, CA) according to manufacturer’s instructions.
Morrissey S.M., Zhang F., Ding C., Montoya-Durango D.E., Hu X., Yang C., Wang Z., Yuan F., Fox M., Zhang H.G., Guo H., Tieri D., Kong M., Watson C.T., Mitchell R.A., Zhang X., McMasters K.M., Huang J, & Yan J. (2021). Tumor-derived Exosomes Drive Immunosuppressive Macrophages in a Pre-metastatic Niche through Glycolytic Dominant Metabolic Reprogramming. Cell metabolism, 33(10), 2040-2058.e10.
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6

Postmortem Blood Biomarker Evaluation

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Briefly, immediately following euthanasia whole blood was removed via 27‐gauge needle from the abdominal aorta, centrifuged at 2,000 g for 2 min in EDTA‐coated tubes. Assessment of plasma lactate was determined using the Eton Bioscience (San Diego, CA, USA) l‐Lactate Assay Kit I Protocol Version 7. Plasma iron level was determined using the Iron‐SL Assay (Sekisui Diagnostics, Lexington, MA, USA) following the manufacturer's protocol. Assessment of plasma triglyceride was determined using the L‐Type TG M Assay (Wako Diagnostics, Mountain View, CA, USA). Assessment of plasma glucose was determined using HemoCue Glucose 201 Systems glucometer.
Moore T.M., Zhou Z., Strumwasser A.R., Cohn W., Lin A.J., Cory K., Whitney K., Ho T., Ho T., Lee J.L., Rucker D.H., Hoang A.N., Widjaja K., Abrishami A.D., Charugundla S., Stiles L., Whitelegge J.P., Turcotte L.P., Wanagat J, & Hevener A.L. (2020). Age‐induced mitochondrial DNA point mutations are inadequate to alter metabolic homeostasis in response to nutrient challenge. Aging Cell, 19(11), e13166.
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7

Cell Line Metabolic Profiling Protocol

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RPMI 1640, F-12K, and Eagle’s minimum essential medium were purchased from American Tissue Culture Collection (Manassas, VA); ChemBridge DIVERSet screening library from ChemBridge Corporation (San Diego, CA); tetramethylrhodamine methylester (TMRM), CellTracker Green, and Hoechst 33342 from Invitrogen (Carlsbad, CA); erastin, nocodazole (NCZ), and paclitaxel (PTX) from Sigma-Aldrich (St. Louis, MO); Microtubules/Tubulin In Vivo Assay Kit from Cytoskeleton (Denver, CO); and L-Lactate Assay Kit I from Eton Bioscience (San Diego, CA). All other reagents were analytical grade.
DeHart D.N., Lemasters J.J, & Maldonado E.N. (2017). Erastin-Like Anti-Warburg Agents Prevent Mitochondrial Depolarization Induced by Free Tubulin and Decrease Lactate Formation in Cancer Cells. SLAS discovery : advancing life sciences R & D, 23(1), 23-33.
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8

Extracellular Glucose and Lactate Assay

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Extracellular glucose and lactate were measured using the glucose assay kit and l-lactate assay kit I (Eton Bioscience) respectively as per the manufacturer’s instructions.
Jones N., Vincent E.E., Cronin J.G., Panetti S., Chambers M., Holm S.R., Owens S.E., Francis N.J., Finlay D.K, & Thornton C.A. (2019). Akt and STAT5 mediate naïve human CD4+ T-cell early metabolic response to TCR stimulation. Nature Communications, 10, 2042.
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9

Extracellular Lactate Quantification

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Extracellular lactate was measured using the l-Lactate Assay Kit I (120001100A; Eton Bioscience). Samples and standards were appropriately diluted and mixed with the l-lactate assay solution and incubated at 37 °C for 30 min. Absorbance was then measured at 490 nm and concentrations calculated from the linear regression of the standard curve.
Jones N., Blagih J., Zani F., Rees A., Hill D.G., Jenkins B.J., Bull C.J., Moreira D., Bantan A.I., Cronin J.G., Avancini D., Jones G.W., Finlay D.K., Vousden K.H., Vincent E.E, & Thornton C.A. (2021). Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation. Nature Communications, 12, 1209.
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10

Evaluating Hyaluronan Production and Lactate Levels in IL-1β-Treated Cells

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Cells were incubated for 12 h with 0.1 ng/ml IL-1β in fresh serum-free culture medium with or without 1.0 mM 4-MU. The culture supernatant fluids were collected and the HA was quantified by enzyme-linked sandwich assay (Hyaluronan DuoSet ELISA, DY3614-05, R&D Systems) following the manufacturer’s instructions. The lactate concentration in culture supernatants was determined using l-Lactate Assay Kit I (Eton Bioscience, Research Triangle, NC, USA) according to the manufacturer’s instructions.
Idota M., Ishizuka S., Hiraiwa H., Yamashita S., Oba H., Kawamura Y., Sakaguchi T., Haga T., Mizuno T., Kawashima I., Kuriyama K, & Imagama S. (2021). 4-Methylumbelliferone suppresses catabolic activation in anterior cruciate ligament-derived cells via a mechanism independent of hyaluronan inhibition. Journal of Orthopaedic Surgery and Research, 16, 507.
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