B6 cg fbn1tsk j

Tsk mice (B6.Cg-Fbn1Tsk/J. stock #014632^{21 (link)} and C57BL/6J (stock # 00064) were purchased (The Jackson Laboratory) and bred in-house. Tsk homozygotes are embryonically lethal and we used Tsk heterozygote mice for our study. Tsk mice and wild-type (WT) littermates as a control from the colony were used 3–15 months (m) of age. Both females and males were used in all experiments. Inter-strain comparisons involved age-matched mice. The mice were raised and housed in air-filtered clear cages with a bedding of pine shavings, subject to a 12 h light/dark cycle, and fed ad libitum. Mice were anesthetized with a mixture of ketamine (85 mg/Kg, Ketaject, Phoenix Pharmaceutical, Inc.), xylazine (8.5 mg/Kg, AnaSed; Lloyd Laboratories) and acepromazine (2.125 mg/Kg, Boehringer Ingelheim), injected intraperitoneally. Anesthesia was titrated to achieve a depth permitting aqueous humor collection. One drop of topical proparacaine hydrochloride ophthalmic solution (0.5%, Akorn Inc.) was applied to the cornea prior to experiments requiring ocular surface contact. Mice were rested on a warming platform or under a heating blanket to maintain body temperature during experiments.