NR8383 cells plated in 24 well plates and treated with IFN-γ or IL-4 for 72 hours. Cells were then detached with Versene (Life Technologies), fixed with 4% paraformaldehyde, washed with PBS, and incubated with FcγR blocking mAb (BD Biosciences, San Jose, CA, USA). Cell membrane permeabilization for intracellular staining was achieved with Permwash solution (BD). Cells were stained with the following antibodies: anti-Nrf2 mouse monoclonal antibody (Abcam, Cambridge, MA, USA), anti-GCLC rabbit polyclonal antibody (Abcam), anti-mouse Pacific Blue (Life Technologies), and anti-rabbit Alexa Fluor 555 (Life Technologies). Data was acquired with an LSR-II flow cytometer in conjunction with FACSDiva software (BD). Mean fluorescence intensities (MFI) were obtained with FlowJo (FlowJo LLC, Ashland, OR, USA).
Anti nrf2 mouse monoclonal antibody
Manufactured by Abcam
Sourced in United States
Anti-Nrf2 mouse monoclonal antibody is a laboratory reagent used to detect the Nrf2 (Nuclear factor erythroid 2-related factor 2) protein in various biological samples. Nrf2 is a transcription factor that regulates the expression of genes involved in the antioxidant response.
Automatically generated - may contain errors
Lab products found in correlation
Versene, by Thermo Fisher Scientific (2 mentions)
Fcγr blocking mab, by BD (2 mentions)
Anti gclc rabbit polyclonal antibody, by Abcam (2 mentions)
Alexa fluor 555 anti rabbit, by Thermo Fisher Scientific (2 mentions)
Perm wash solution, by BD (2 mentions)
Facsdiva software, by BD (2 mentions)
Lsrii flow cytometer, by BD (2 mentions)
2 protocols using anti nrf2 mouse monoclonal antibody
1
Quantifying Nrf2 and GCLC in IFN-γ and IL-4 treated NR8383 cells
2
Quantifying Nrf2 and GCLC in IFN-γ and IL-4 treated NR8383 cells
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NR8383 cells plated in 24 well plates and treated with IFN-γ or IL-4 for 72 hours. Cells were then detached with Versene (Life Technologies), fixed with 4% paraformaldehyde, washed with PBS, and incubated with FcγR blocking mAb (BD Biosciences, San Jose, CA, USA). Cell membrane permeabilization for intracellular staining was achieved with Permwash solution (BD). Cells were stained with the following antibodies: anti-Nrf2 mouse monoclonal antibody (Abcam, Cambridge, MA, USA), anti-GCLC rabbit polyclonal antibody (Abcam), anti-mouse Pacific Blue (Life Technologies), and anti-rabbit Alexa Fluor 555 (Life Technologies). Data was acquired with an LSR-II flow cytometer in conjunction with FACSDiva software (BD). Mean fluorescence intensities (MFI) were obtained with FlowJo (FlowJo LLC, Ashland, OR, USA).
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