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Ipgell kit

Manufactured by Genostaff
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Sourced in Japan

The IPGell kit is a laboratory equipment used for isoelectric focusing, a technique to separate proteins based on their isoelectric point. The kit includes all the necessary components to perform this analysis.

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IPGell (34 citations)

2 protocols using ipgell kit

1

Harvesting and Analyzing HepaMN Cells

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HepaMN cells were harvested with a cell scraper and collected into tubes. The cells were analyzed with an iPGell kit (GenoStaff, Tokyo, Japan).
Nishiwaki M., Toyoda M., Oishi Y., Ishida S., Horiuchi S.I., Makino-Itou H., Kimura T., Ohno S.I., Ohkura T., Enosawa S., Akutsu H., Nakazawa A., Kasahara M., Kiyono T, & Umezawa A. (2020). Immortalization of human hepatocytes from biliary atresia with CDK4R24C, cyclin D1, and TERT for cytochrome P450 induction testing. Scientific Reports, 10, 17503.
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2

Cell Fixation and Histological Analysis

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Cells were harvested with a cell scraper and collected into tubes. The cells were fixed in 4% paraformaldehyde for 10 min at 4 °C. The cells were analyzed with an iPGell kit (GenoStaff, Tokyo, Japan). Paraffin-embedded tissue was sliced and stained with hematoxylin and eosin.
Miyata S., Saku N., Akiyama S., Javaregowda P.K., Ite K., Takashima N., Toyoda M., Yura K., Kimura T., Nishina H., Nakazawa A., Kasahara M., Nonaka H., Kiyono T, & Umezawa A. (2022). Puromycin-based purification of cells with high expression of the cytochrome P450 CYP3A4 gene from a patient with drug-induced liver injury (DILI). Stem Cell Research & Therapy, 13, 6.
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