Alcl3

Manufactured by Fujifilm

Sourced in Japan

AlCl3, or aluminum chloride, is a chemical compound used in various laboratory applications. It serves as a versatile Lewis acid catalyst, facilitating a wide range of chemical reactions. Its primary function is to catalyze organic synthesis processes.

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Lab products found in correlation

Diisopropyl ether, by Fujifilm (1 mentions) Tetrahydrofuran thf, by Fujifilm (1 mentions) Sodium chloride (nacl), by Kanto Chemical (1 mentions) Penicillin, by Nacalai Tesque (1 mentions) Streptomycin, by Nacalai Tesque (1 mentions) Fecl3, by Fujifilm (1 mentions) Cocl2, by Fujifilm (1 mentions) Cuso4, by Fujifilm (1 mentions) Nicl2, by Fujifilm (1 mentions) Zncl2, by Fujifilm (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions)

3 protocols using alcl3

Synthesis and Characterization of Magnesium Salt

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Example 15

(1) Synthesis of a Magnesium Salt

Under argon gas atmosphere, diphenylsilanediol (8.65 g, 40 mmol) (Tokyo Chemical Industry Co., Ltd.) was dissolved in tetrahydrofuran (THF) (20 mL) (Wako Pure Chemical Industries, Ltd.), and then a THF solution of phenylmagnesium chloride (PhMgCl) (40 mL, 80 mmol) (Tokyo Chemical Industry Co., Ltd., 2 M) was added dropwise, and the solution was stirred for 1 hour. After that, powder generated by concentration and drying of the solution, was washed with diisopropyl ether (50 mL) (Wako Pure Chemical Industries, Ltd.). The powder was filtered off and dried to obtain diphenylsilanedioxy bis(magnesium chloride), (Ph₂Si(OMgCl)₂).

[Figure (not displayed)]

Measurement result of ¹H-NMR is shown below.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 6.90-8.00 (m, 10H)

(2) Preparation of the Electrolytic Solution

Under argon gas atmosphere, into diphenylsilanedioxy bis(magnesium chloride) (Ph₂Si(OMgCl)₂) (0.83 g, 2.5 mmol), THF (20 mL) (Wako Pure Chemical Industries, Ltd.) was mixed. The mixture was heated to 50° C., and then aluminum chloride (AlCl₃) (0.67 g, 5 mmol) (Wako Pure Chemical Industries, Ltd.) was added to the mixture. After maintaining the resulting mixture at 50° C. for 10 minutes, it was cooled to obtain the electrolytic solution 14, “a solution of diphenylsilanedioxy bis(magnesium chloride)-aluminum chloride/THF”.

US10367231B2. Magnesium-containing electrolytic solution (2019-07-30). FUJIFILM Wako Pure Chemical Corporation [JP]. Inventors: Kazuhiko Sato [JP], Takahiro Kiyosu [JP], Hironori Mizuta [JP], Goro Mori [JP], Kuniaki Okamoto [JP].

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Quantifying Stress Tolerance of Microbial Isolates

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To assess the tolerance of isolates to different NaCl concentrations, 5 μL of the culture solutions (10⁹ cells mL⁻¹) of isolates was incubated on YMA supplemented with 0 (the control), 1, 2, 3, or 4% (w/v) NaCl (Kanto Chemical, Tokyo, Japan). Plates were incubated at 28°C for 4–5 d (22 (link)), and the growth rate of each colony was scored as follows: no growth (−), 10–20% more growth than the control (+), 30–60% more growth than the control (++), and 70–100% more growth than the control (+++).
To assess the Al tolerance of isolates, 5 μL of the culture solution (10⁹ cells mL⁻¹) of isolates was incubated on YMA supplemented with AlCl₃ (Wako Pure Chemical Industries, Osaka, Japan) concentrations of 0.1 (as the control), 0.5, 1, or 2 mM in combination with acidic (pH 4.5) and neutral pH (pH 6.8). The plates were incubated at 28°C for 4–15 d (22 (link), 45 (link)). The evaluation of the Al tolerance of isolates used the same scoring method as that for NaCl tolerance. Experiments were performed in triplicate.

Ramírez M.D., España M., Aguirre C., Kojima K., Ohkama-Ohtsu N., Sekimoto H, & Yokoyama T. (2019). Burkholderia and Paraburkholderia are Predominant Soybean Rhizobial Genera in Venezuelan Soils in Different Climatic and Topographical Regions. Microbes and Environments, 34(1), 43-58.

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Metal Exposure Effects on HepG2 Cells

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The cell lines present in this study were obtained from RIKEN BRC CELL BANK. HepG2 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, GIBCO) supplemented with 10% fetal bovine serum (FBS, Biowest), 100 U mL⁻¹ penicillin, and 100 U mL⁻¹ streptomycin (Nacalai Tesque) at 37°C in a 5% CO₂ atmosphere. The metal exposure was performed as follows: 2 × 10⁴ cells were seeded in a 3.5-cm culture dish and first cultured with normal medium for 48 h; subsequently, they were washed with phosphate-buffered saline (PBS) and then treated with medium including FeCl₃, AlCl₃, CoCl₂, CuSO₄, NiCl₂ and ZnCl₂ (FUJIFILM Wako Pure Chemical Corporation), ranging from 20 to 200 μM, separately. After incubation for 24 h, the cells were fixed using the following steps. First, cells were digested with .25% trypsin/EDTA, followed by PBS washing. Then, the cells were gently suspended in a hypotonic solution (75 mM KCl) and allowed to stand for 8 min at room temperature. After the same volume of Carnoy’s solution (fixative solution, methanol/acetic acid (3/1, v/v)) was added, cells were mixed gently. After centrifugation at 1,500 rpm, the supernatant was discarded, and an equal volume of fresh Carnoy’s solution was added. This procedure was repeated twice. Fixed cells were stored in Carnoy’s solution at −20°C.

Liu S., Morihiro K., Takeuchi F., Li Y, & Okamoto A. (2023). Interstrand crosslinking oligonucleotides elucidate the effect of metal ions on the methylation status of repetitive DNA elements. Frontiers in Chemistry, 11, 1122474.

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