The levels of alanine aminotransferase (ALT) (ab234579, Abcam Inc., Cambridge, MA, USA), aspartate aminotransferase (AST) (ab263883, Abcam), IL‐6 (ab178013, Abcam), TNF‐α (ab181421, Abcam), IL‐1β (ab217608, Abcam) and IL‐18 (ab215539, Abcam) was detected using ELISA kits. Briefly, the sample to be tested (containing antibody) was bound to antigen, and then, the labelled enzyme was bound to the complex to form antigen‐antibody‐labelled enzyme complex. The enzyme substrate was added to produce coloured product, and its optical density value was determined by spectrophotometer.
Ab263883
Manufactured by Abcam
Sourced in United States, United Kingdom
Ab263883 is a laboratory reagent. It is a monoclonal antibody directed against a specific target molecule. The core function of this product is to detect and bind to the target molecule for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Ab234579, by Abcam (7 mentions)
Ab108789, by Abcam (3 mentions)
Mbs730053, by MyBioSource (2 mentions)
Ab178013, by Abcam (1 mentions)
Ab217608, by Abcam (1 mentions)
Ab215539, by Abcam (1 mentions)
Ab181421, by Abcam (1 mentions)
Total protein, by Bio-Rad (1 mentions)
Ab83369, by Abcam (1 mentions)
Ab105134 k752 100, by Abcam (1 mentions)
Ab102526, by Abcam (1 mentions)
Csb e04494r, by Cusabio (1 mentions)
Glutathione peroxidase, by Biodiagnostic (1 mentions)
Ab255730, by Abcam (1 mentions)
Ab236712, by Abcam (1 mentions)
Ab214566, by Abcam (1 mentions)
Ab234570, by Abcam (1 mentions)
Mak066, by Merck Group (1 mentions)
Mak085, by Merck Group (1 mentions)
S0024, by Beyotime (1 mentions)
9 protocols using ab263883
1
Quantifying Inflammatory Markers via ELISA
2
Serum Biomarker Quantification
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Serum was utilized to measure the levels of ALT, AST, and albumin by the ab234579, ab263883, and ab108789 kits purchased from Abcam, USA. In addition, the total proteins (BioRad, Hercules, CA, USA) and bilirubin (MBS730053, MyBioSource, San Diego, CA, USA) were measured in all experimental groups.
3
Serum-based Liver Function Assessment
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Serum was utilized, in the current study, for assessment of liver function using commercial kits of ELISA. The levels of alanine aminotransferase (ALT) (ab234579, Abcam, Cambridge, UK), aspartate aminotransferase (AST) (ab263883, Abcam, Cambridge, UK), albumin (ab108789, Abcam, Cambridge, UK), total proteins (BioRad, Hercules, CA, USA), and bilirubin (MBS730053, MyBioSource, San Diego, CA, USA) were measured in all groups [24 (link)].
4
Blood Biomarker Analysis in Anesthetized Animals
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At the end of the reperfusion phase, blood samples were harvested from the abdominal aorta of the anesthetized animals, and the sera were isolated for the measurement of alanine aminotransferase (ALT, ab105134/K752-100), aspartate aminotransferase (AST, ab263883), alkaline phosphatase (ALP, ab83369), and lactate dehydrogenase (LDH, ab102526) using diagnostic kits obtained from Abcam (Boston, MA, USA).
5
Plasma Biomarkers Quantification Protocol
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Plasma levels of ACE (MBS2020292, MyBioSource, San Diego, CA, USA), AngII (CSB-E04494r, Cusabio, Houston, TX, USA), ALT (ab234579, Abcam, Waltham, MA, USA) and AST (ab263883, Abcam, Waltham, MA, USA) were measured via ELISA according to manufacturer’s instructions by a blinded researcher.
6
Doxorubicin-Induced Cardiotoxicity: Evaluation Protocol
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DOX was obtained as an injectable commercial product (Adriblastina vials, Pharmacia Italia S.P.A., Italy). Each vial has 50 mg DOX hydrochloride (dried powder) and kept at 4°C. Before use, each vial's contents were dissolved in 25 ml of sterile saline solution so each ml contains 2 mg DOX.
HES (powder form) was obtained from Sigma Aldrich (St. Louis, MO, USA), kept at 2°C–4°C and freshly dissolved in distilled water just before use.
Kits for Superoxide dismutase (SOD), Catalase (CAT), and Glutathione peroxidase (GPX) were purchased from Biodiagnostic Company, Giza, Egypt.
In addition, Cardiac markers assessment Kits; Troponin I (CTnI) ELISA Kits were obtained from IMMUNOSPEC Corporation, USA, CAT. No. E29-061. Creatine Kinase Activity (CK) (ab155901), Creatine Kinase MB (CK-MB) (ab285275), and Aspartate Aminotransferase (AST) (ab263883) kits were purchased from Abcam, UK. Lactate dehydrogenase enzyme (LDH) kits were purchased from the Egyptian Company of Biotechnology, EGYPT.
ELISA Kits for the exact measurement of Interleukin 1β (IL-1β) Cat. No. E0094Hu, Tumor necrosis factor α (TNF-α) Cat. No. E0082Hu and Interferon γ (IFN-γ) Cat. No. E0105Hu were purchased from Bioassay Technology Laboratory Company, China.
HES (powder form) was obtained from Sigma Aldrich (St. Louis, MO, USA), kept at 2°C–4°C and freshly dissolved in distilled water just before use.
Kits for Superoxide dismutase (SOD), Catalase (CAT), and Glutathione peroxidase (GPX) were purchased from Biodiagnostic Company, Giza, Egypt.
In addition, Cardiac markers assessment Kits; Troponin I (CTnI) ELISA Kits were obtained from IMMUNOSPEC Corporation, USA, CAT. No. E29-061. Creatine Kinase Activity (CK) (ab155901), Creatine Kinase MB (CK-MB) (ab285275), and Aspartate Aminotransferase (AST) (ab263883) kits were purchased from Abcam, UK. Lactate dehydrogenase enzyme (LDH) kits were purchased from the Egyptian Company of Biotechnology, EGYPT.
ELISA Kits for the exact measurement of Interleukin 1β (IL-1β) Cat. No. E0094Hu, Tumor necrosis factor α (TNF-α) Cat. No. E0082Hu and Interferon γ (IFN-γ) Cat. No. E0105Hu were purchased from Bioassay Technology Laboratory Company, China.
7
Liver Function and Inflammatory Markers
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Enzyme-linked immunosorbent assay (ELISA) kits of AST (ab263883, abcam, USA), ALT (ab234579, abcam, USA), tumor necrosis factor (TNF)-α (ab236712, abcam, USA), interleukin (IL)-1β (ab255730, abcam, USA), IL-6 (ab234570, abcam, USA) and IL-10 (ab214566, abcam, USA), and LDH kit (MAK066, Merck, German) were used to detect the levels of liver function markers and inflammatory factors in the serum according to the manufacturer’s instructions.
After washing the liver with a normal saline solution, 100 mg of liver tissue was cut and made into a 10% homogenate. The homogenate was centrifuged at 2,500 r/minute for 15 minutes to obtain the supernatant. The malondialdehyde (MDA) kit (MAK085, Merck, German), superoxide dismutase (SOD) kit (MM-0386R2, MEIMIAN, China), and nitric oxide (NO) kit (S0024, Beyotime, China) were used to detect the levels of MDA, SOD, and NO, respectively, in the supernatant.
After washing the liver with a normal saline solution, 100 mg of liver tissue was cut and made into a 10% homogenate. The homogenate was centrifuged at 2,500 r/minute for 15 minutes to obtain the supernatant. The malondialdehyde (MDA) kit (MAK085, Merck, German), superoxide dismutase (SOD) kit (MM-0386R2, MEIMIAN, China), and nitric oxide (NO) kit (S0024, Beyotime, China) were used to detect the levels of MDA, SOD, and NO, respectively, in the supernatant.
8
Evaluating Hepatoprotective Effects of Mel-PLGA NPs
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Liver function parameters were measured in order to evaluate the hepatoprotective effect of Mel-PLGA NPs. The levels of aspartate aminotransferase (AST), alanine transaminase (ALT), albumin (ab234579, ab263883, ab108789, abcam, USA) and total bilirubin (MBS9389057, MyBiosource, USA) were measured by rat ELISA kits according to Farid et al.27 .
9
Quantifying Cardiac Biomarkers
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Corresponding enzyme-linked immunosorbent assay kits (ab246529, ab285275, ab263883, and ab234579; Abcam) were employed to measure the contents of Troponin I, creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). The aforementioned biochemical indexes were measured strictly per the specific operation steps and cautions in the protocols of the corresponding kits. The optical density was analyzed and calculated with a microplate reader (Beckman Coulter, CX7, Brea, CA, USA) and the concentrations of the samples were calculated based on the corresponding formula or standard curve and regression equation of biomarkers.
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