The ZnO NPs (diameter = 50 nm) were purchased from Nanjing Haitai Nanoparticles Ltd. The stock solution of ZnO NPs (0.5 g/L) was prepared before the exposure treatments. Nanoparticles with a diameter around 25 nm were suspended in Milli-Q water, which was further processed in an ice-water bath for half an hour using the ultrasonic vibration at 40 kHz. The concentration of the stock ZnO NPs solution was 0.5 g/L, which was then diluted to six levels, including 0 mg/L, 5 mg/L, 10 mg/L, 20 mg/L, 50 mg/L, and 100 mg/L. In order to assess the morphological configuration of ZnO NPs, H-7650 scanning electron microscopy (SEM, Hitachi, H-7650, Tokyo, Japan) was employed for the suspension.
Zno nps
Manufactured by Hitachi
Sourced in Japan
ZnO NPs are zinc oxide nanoparticles. They are a type of engineered nanomaterial with a size range between 1 to 100 nanometers. ZnO NPs exhibit unique physical and chemical properties due to their nano-scale dimensions.
Automatically generated - may contain errors
Lab products found in correlation
H 7500, by Hitachi (2 mentions)
Zno np, by Merck Group (2 mentions)
H 7650, by Hitachi (2 mentions)
D8 advance x ray powder diffractometer, by Bruker (1 mentions)
S 3000n scanning electron microscope, by Hitachi (1 mentions)
Zetapals, by Brookhaven Instruments (1 mentions)
S 4700, by Hitachi (1 mentions)
S 4800 fesem, by Hitachi (1 mentions)
Ultima 4, by Rigaku (1 mentions)
S 4800 field, by Hitachi (1 mentions)
7 protocols using zno nps
1
Characterization of ZnO Nanoparticles
2
Comprehensive Characterization of ZnO Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ZnO NPs (about 20 nm) were obtained from Beijing DK nanotechnology Co. Ltd. The size, shape, and dispersion of ZnO NPs were evaluated by transmission electron microscopy (TEM, H-7500; Hitachi Ltd., Tokyo, Japan). X-ray diffraction (XRD) data were collected on aD8 Advance X-ray Powder Diffractometer (Bruker Optik GmbH, Ettlingen, Germany). Ultraviolet-visible (UV-vis) spectra were recorded using an OPTIZEN spectrophotometer (Hitachi Ltd.). The surface chemical bonding and composition of NPs were characterized using a Fourier transform infrared spectroscopy (FTIR) instrument (Spectroscopy GX; PerkinElmer Inc., Branford, CT, USA). Atomic force microscopy (AFM) was used for evaluating the surface morphology and properties of the ZnO NPs.
3
Characterization of ZnO Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were carried out on an ultraclean bench to prevent interference of external factors. Highly purified (99.9%) ZnO NPs were purchased from Sigma Aldrich (St. Louis, USA). Stock solutions of ZnO NPs were prepared in Dulbecco's modified Eagle's medium (DMEM) containing 50 μg/mL fetal bovine serum (FBS). To avoid particle aggregation, the prepared solutions were sonicated three times (20 s/time) prior to use [10 (link), 11 (link)]. ZnO NPs in DMEM were characterized in terms of morphology, diameter, tendency of aggregation, and intracellular distribution using a scanning electron microscope (SEM, Hitachi S-4800, Japan). Zeta potential analysis of ZnO NPs in DMEM was performed by using dynamic light scattering (Malvern Zetasizer ZS9, Worcestershire, UK).
4
Characterization of ZnO Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ZnO NPs (about 70 nm) were obtained from Beijing DK nanotechnology Co. Ltd (Beijing, People’s Republic of China). The size, shape, and dispersion of ZnO NPs were evaluated by transmission electron microscopy (TEM) (H-7500; Hitachi Ltd., Tokyo, Japan) and dynamic light scattering (DLS) (ZetaPals; Brookhaven Instruments Corporation, Long Island, NY, USA). X-ray diffraction (XRD) data were collected on a D8 Advance X-ray Powder Diffractometer (Bruker Optik GmbH, Ettlingen, Germany). The specimens were examined using an S-3000N scanning electron microscope (SEM) system (Hitachi Ltd.) operated at 15 kV. The surface chemical bonding and composition of NPs were characterized using a Fourier transform infrared spectroscopy (FTIR) instrument (PerkinElmer Spectroscopy GX, Branford, CT, USA). Atomic force microscopy (AFM) was used for evaluating the surface morphology and properties of the ZnO NPs.
5
Characterization of ZnO Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ZnO-NPs were purchased from UniRegion Bio-Tech (New Taipei City, Taiwan), and the morphology of ZnO-NPs was observed under a scanning electron microscope (SEM; Hitachi S-4700) with an accelerating voltage of 15 kV (Figure S3 ). Particles were coated with a platinum film by sputtering physical vapor deposition. ZnO-NPs were dispersed in dimethyl sulfoxide (DMSO), without any surfactant or modification, for all assays. DMSO was used as the vehicle control in the experiments.
6
Characterization of ZnO Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zinc oxide nanoparticles (ZnO NPs) were obtained from Sigma-Aldrich in the USA with the particle size ≤50 nm. The ultrastructure and mean size of 1 mM ZnO NPs were analyzed by transmission electron microscope (Hitachi H-7650, Japan). The hydrodynamic diameter and zeta potential of ZnO NPs at 1 mM in double distilled water and cell culture medium were, respectively, determined by dynamic light scattering method using Nano Brook 90Plus Zeta (Brookhaven, USA).
7
Characterization of ZnO Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ZnO NPs were purchased from Shanghai Aladdin Reagent Co., Ltd, with a purity of 99.5 % and particle size of 30± 10 nm provided by the producer. The size of ZnO NPs measured in our laboratory using a Hitachi S-4800 Field Emission Scanning Electron Microscope (Hitachi S-4800 FE-SEM) was 30±12 nm (Supplementary Material Fig. S1). X-ray difffaction analysis (XRD, Rigaku Ultima IV) of the nanoparticles revealed a pure ZnO minerals composed of zincite (Supplementary Material Fig. S2). The specific surface area determined using the multi-point Brunauer-Emmett-Teller (BET) method was 42.0 m 2 g -1 . All other chemicals of analytical reagent grade were purchased from Sinopharm Chemical Regent Co., Ltd, China.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!