3-Carboxy-5-nitrophenylboronic acid pinacol ester was obtained from Combi-Blocks (San Diego, CA). Monomethylterminated poly ethylene glycol monoamine (MeO-PEG-NH2, Mw: 5000 Da) was purchased from Rapp Polymere (Tübingen, Germany). PTX was purchased from AK Scientific Inc. (Mountain View, CA). Taxol (Mayne Pharma Paramus, NJ) was obtained from the Comprehensive Cancer Center of University of California, Davis. (Fmoc)lys(Boc)eOH, (Fmoc)Lys(Dde)eOH, (Fmoc)Lys(Fmoc)eOH, and (Fmoc) Ebes-OH were obtained from AnaSpec Inc. (San Jose, CA). 1,1 ’-dioctadecyl-3, 3, 3’, 3’-tetramethylindodicarbocyanine perchlorate (DiD), DiOC18 (DiO) and the red-orange dye rhodamine B were purchased from Invitrogen (Carlsbad, CA). Cholic acid and all other chemicals were purchased from Sigma Aldrich (St. Louis, MO).
Fmoc ebes oh
Manufactured by AnaSpec
The (Fmoc) Ebes-OH is a chemical reagent used in solid-phase peptide synthesis. It is a protecting group that can be selectively removed to facilitate the coupling of the next amino acid during the synthesis process.
Automatically generated - may contain errors
2 protocols using fmoc ebes oh
1
Synthesis of Polymer-Drug Conjugates
2
Synthesis and Purification of TAMRA-Labeled Peptides
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Rink amide MBHA resin LL and all Fmoc-protected amino acids were purchased from Novabiochem. Fmoc-Ebes-OH was purchased from Anaspec. N-Methyl-2-pyrrolidone was purchased from Merck. Other solvents were purchased from Sigma-Aldrich. Peptide was synthesized on Rink Amide MBHA resin LL using standard Fmoc protocols via a Tribute™ peptide synthesizer (Protein Technologies, Inc.). The peptides were purified by reverse-phase high-performance liquid chromatography (HPLC, water-acetonitrile with 0.1 % TFA). Concentration was determined spectrophotometrically using a molar extinction coefficient of TAMRA (80,400 M−1 cm−1) at 547 nm.
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