Ion personal genome machine hi q sequencing kit

DNA libraries were prepared according to the Ampliseq Library Preparation Kit 2.0—96Lv (Thermo Fisher, San Diego, USA, MAN0006735) standard protocol using 20 ng of input DNA, with samples barcoded using Ion Xpress Barcodes 1–96 (Thermo Fisher). Prepared libraries were diluted to 26 pM using MilliQ water and combined into four samples pools, for sequencing on four 318 chips. Twenty-five microliters of each sample pool was enriched using a One-Touch Two (OT2) machine and automated enrichment system (Thermo Fisher) with the Ion PGM^™ Template OT2 400 Kit (Thermo Fisher) to prepare Template-positive Ion Sphere Particles. Enriched products were prepared for sequencing using the Ion Personal Genome Machine^™ (PGM) Hi-Q Sequencing Kit and run on the Personal Genome Machine using the Ion 318 chip v2 (Thermo Fisher, MAN0009816). In parallel, libraries for each sample were diluted to 70 pM and loaded onto an Ion Chef for sequencing on an Ion Torrent S5 XL^™ using an Ion 530 chip (Thermo Fisher, MAN0010851) (Fig 1).