Standard rodent pellet diet

Adult male 3-month-old C57BL/6 J mice (27–30 g) were obtained from Envigo (Barcelona, Spain), while Tg (TcraTcrb)425Cbn (OT-II) mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). The mice were housed at 21 °C in a humidity controlled environment on a 12-h light/dark cycle, fed ad libitum with standard rodent pellet diet (Envigo, Barcelona, Spain) and free access to water. Wild type animals received one intraperitoneal administration of LPS (O111:B4; 5 mg/kg in saline; Sigma-Aldrich, St. Louis, MO, USA) or saline alone, and they were sacrificed 48 h later. All procedures involving animals were carried out in accordance with the Spanish National Research Council’s guide for the care and use of laboratory animals, and the experimental design was approved by the Ethical Committee for Animal Testing at the University of Navarra (ref. 109-18).

Adult male 3 or 4-month-old C57BL6J (20–30 g) mice were obtained from Envigo (Barcelona, Spain). Mice were housed at 21 °C on a 12 h light/dark cycle in a humidity-controlled environment with ad libitum access to food with standard rodent pellet diet (Envigo) and free access to water. All procedures involving animals were carried out in accordance with the Spanish National Research Council’s guide for the care and use of laboratory animals. The experimental designs were approved by the Ethical Committee for Animal Testing at the University of Navarra (Ref. 109 − 18).