Infinitylab poroshell 120 ec c18

Manufactured by Agilent Technologies

Sourced in United States

The InfinityLab Poroshell 120 EC-C18 is a high-performance liquid chromatography (HPLC) column designed for efficient and reliable separation of a wide range of analytes. It features a 120 Å porous silica particle structure and a C18 stationary phase, providing consistent and reproducible chromatographic results.

Automatically generated - may contain errors

Lab products found in correlation

19 protocols using infinitylab poroshell 120 ec c18

Quantification of PET and Nylon Hydrolysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The release products after enzymatic hydrolysis of PET and nylon were measured via HPLC. To remove protein impurities from the sample, a methanol precipitation step was performed with ice cold methanol (1:1 sample/methanol) (Quartinello et al., 2017 (link)). Afterwards, samples were centrifuged at 12700 rpm for 15 min. at 4°C (5920 R Centrifuge from Eppendorf) followed by an acidification step with 6 N HCl (6 μL per sample). Before pipetting the samples into the HPLC-vials, a filtration step with a 0.45 μM PA filter was performed. For the measurements of the PET released product (Ta), an Agilent LC-MS system was used with a Poroshell 120 column (InfinityLab Poroshell 120 EC-C18, 4.6 × 50 mm, 4 μM, Agilent), a flow of 0.35 mL/min and a non-linear gradient (Table S1). The released products were detected at 241 nm via UV-Vis spectroscopy. Caprolactam released from nylon-6 was measured using an Agilent LC-MS system with a phenomex® column (Aqua® 5 μm C18, 125 Å, LC Column, 250 x 4.6 mm) with an isocratic gradient (H₂O/MeOH, 60/40 [% v/v]) and flow of 0.5 mL/min for 50 min. Hydrolysates were measured at 210 nm via UV-Vis spectroscopy.

Quartinello F., Kremser K., Vecchiato S., Schoen H., Vielnascher R., Ploszczanski L., Pellis A, & Guebitz G.M. (2019). Increased Flame Retardancy of Enzymatic Functionalized PET and Nylon Fabrics via DNA Immobilization. Frontiers in Chemistry, 7, 685.

+ Open protocol

+ Expand

Etoposide-Loaded Uro-Functionalized Sphingomyelin Nanosystems

Check if the same lab product or an alternative is used in the 5 most similar protocols

UroGm-functionalized sphingomyelin nanosystems (UroGm-SNs) were additionally loaded with the chemotherapeutic drug etoposide (UroGm-Etp-SNs). In this case, up to 250 µg of etoposide (40 mg/mL in DMSO) were placed into the organic phase within the 50 µL of ethanol and injected into the 450 µL of ultrapure water containing UroGm. Nanosystems were isolated using the same conditions as previously described. Encapsulation efficiency was determined by direct quantification of etoposide in the nanosystem using an isocratic HPLC method optimized from the literature^{57 (link)}. Analyses were performed in an HPLC system 1260 Infinity II Agilent (Agilent Technologies, USA) equipped with a pump G7111A, an autosampler G7129A and an UV–Vis detector G7114A set at 254 nm. Separation was achieved on an InfinityLab Poroshell 120 EC-C18 (100 mm × 4.6 mm, 4 µm pore size) Agilent column. The mobile phases were composed of water and acetonitrile (H₂O:ACN,70:30 v/v) at a flow rate of 1 mL/min. Standard calibration curves were linear in the range of 1 to 15 µg/mL (R² = 0.9999) (Limit of quantification, LOQ = 1 ppm).

Bouzo B.L., Lores S., Jatal R., Alijas S., Alonso M.J., Conejos-Sánchez I, & de la Fuente M. (2021). Sphingomyelin nanosystems loaded with uroguanylin and etoposide for treating metastatic colorectal cancer. Scientific Reports, 11, 17213.

+ Open protocol

+ Expand

Quantification of Polyhydroxybutyrate by HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The quantification of PHB was performed by measuring the crotonic acid absorbance at 215 nm following HPLC. From 20 mL of cell culture, the pellet was harvested by centrifugation (15,317× g, 10 min, 4 °C) and washed two times with equal volumes of acetone and ethanol. The conversion of PHB to crotonic acid was completed after digestion of the pellet in 1 mL of concentrated sulfuric acid (Merck, Darmstadt, Germany) for 30 min at 105 °C. After the digestion, the samples were diluted with nanopure H₂O in a 1:5 volume ratio and filtered using 0.22 μm filters. The filtered samples were analyzed by the Agilent 1260 Infinity II LC System (Agilent, Santa Clara, CA, USA). The samples were loaded on the reversed-phase column InfinityLab Poroshell 120 EC-C18 (4 μm pore size, 4.6 × 150 mm, Agilent, Santa Clara, USA) and eluted with 0.5 mL/min, 85% phosphoric acid solution (0.1 M, Honeywell, NC, USA), and 15% (v/v) acetonitrile (Fisher Scientific, Portsmouth, NH, USA), at 30 °C. Furthermore, the crotonic acid in the samples was detected by a diode array detector at 215 nm and quantified based on a standard curve.

Drakonaki A., Mathioudaki E., Geladas E.D., Konsolaki E., Vitsaxakis N., Chaniotakis N., Xie H, & Tsiotis G. (2023). Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source. Microorganisms, 11(6), 1592.

+ Open protocol

+ Expand

Cecum Metabolite Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

20 µL of supernatant from each cecum sample and standard pool dilution were reacted with 20 µL of 200 mM N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride in 5% pyridine and 40 µL of 100 mM 2-nitrophenylhydrazine in 80% ACN/H₂O (v/v) with 50 mM HCl. Mixtures were incubated for 30 min at 40 °C before adding 400 µL of 10% ACN/H₂O (v/v) to each sample. Samples were centrifuged and transferred into a 96-well injection plate for triple quadrupole LC-MS/MS analysis (using Agilent 6490 triple quadrupole mass spectrometer equipped with Agilent 1290 infinity LC system, and an Agilent InfinityLab Poroshell 120 EC-C18, 2.1 × 100 mm, 1.9 µm column). The samples were detected in positive mode using a dynamic multiple reaction monitoring MRM method.

Lee S., Goodson M., Vang W., Kalanetra K., Barile D, & Raybould H. (2020). 2′-Fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice. Nutrients, 12(4), 1003.

+ Open protocol

+ Expand

Cannabinoid Quantification by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

The extracted cannabinoid samples were analysed using LC-MS (1260 infinity liquid chromatograph coupled to Agilent 6470 tri quadrupole mass spectrometer) equipped with reverse phase C18 column (InfinityLab Poroshell 120 EC-C18, 3.0 × 100 mm 2.7-Micron, Agilent). The mobile phase was set as solvent A (Water with 0.05 % formic acid) and solvent B (acetonitrile with 0.05 % formic acid). The compounds were separated via gradient elution as follows: linearly increased from 30 % B to 40 % B in 3.0 min, then increased to 80 % B in 4.8 min, increased from 80 % B to 97 % B in 3.0 min, held at 97 % B for 2.0 min, decreased from 97 % B to 30 % B in 0.1 min, and held at 30 % B for 2.1 min. The flow rate was held at 0.5 ml min⁻¹ and total liquid chromatography run time was 15.0 min. The sample panel and column compartment were set at 15 °C and 40 °C, respectively. The tri quadrupole mass spectrometer was set as follows: gas temperature 300 °C, gas flow 5 L min⁻¹, nebuliser 45 psi, sheath gas temperature 250 °C, sheath gas flow 11 L min⁻¹. Electrospray ionisation was conducted in the negative/positive ion mode and capillary voltage of 3500 V as used. The data files were processed with Agilent MassHunter Qualitative Analysis software.

Shaw W.M., Zhang Y., Lu X., Khalil A.S., Ladds G., Luo X, & Ellis T. (2022). Screening microbially produced Δ9-tetrahydrocannabinol using a yeast biosensor workflow. Nature Communications, 13, 5509.

+ Open protocol

+ Expand

Quantitative Analysis of Ciclesonide by RP-HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quantitation of Ciclesonide was done by reversed phase high performance liquid chromatography (RP-HPLC) with UV detection. An Agilent 1260 Infinity Series module HPLC system equipped with a UV detector (Agilent Technologies, Santa Clara, CA, USA) was used. Separation was achieved with an Agilent InfinityLab Poroshell 120 EC-C18, 3.0 × 150 mm, 2.7 μm column (P/N 693975-302) maintained at 40°C and gradient separation using water:trifluoroacetic acid (0.025% v/v) and acetonitrile:trifluoroacetic acid (0.025% v/v) operated at 0.6 mL/min. The autosampler was maintained at 2–8 °C and a 40 μL injection volume was used. Ciclesonide detection was performed at a wavelength of 242 ± 2 nm. Quantitation was performed by comparison to an external standard. Method linearity was established across a quantitation range from 0.08 to 200 μg/mL.

Miller D.P., Tarara T.E, & Weers J.G. (2021). Targeting of Inhaled Therapeutics to the Small Airways: Nanoleucine Carrier Formulations. Pharmaceutics, 13(11), 1855.

+ Open protocol

+ Expand

Comprehensive Untargeted Metabolomics of Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots (40 µL for (positive mode) and 120 µL for (negative mode)) of thawed plasma (NIST SRM 1950 Metabolites in Frozen Plasma, Sigma, St. Louis USA) were each extracted using a modified Folch extraction procedure [36 (link)] and reconstituted in 100 µL of a methanol/chloroform mixture (9:1, v/v). LC separation was performed on an Agilent 1290 Infinity II LC System, with a 19 min gradient time on a reverse phase C18 column (Agilent InfinityLab Poroshell 120 EC-C18, 3.0 × 100 mm, 2.7 µm). Mobile phase consisted of 10 mM ammonium acetate and 0.2 mM ammonium fluoride in 9:1 water/methanol, while mobile phase B consisted of 10 mM ammonium acetate and 0.2 mM ammonium fluoride in 2:3:5 acetonitrile/methanol/isopropanol. Negative and positive polarity data was acquired on the Agilent 6546 LC/Q-TOF using iterative MS/MS acquisition mode on 6 injections of extracted plasma for each polarity [37 ]. Detailed experimental methods for chromatography and mass spectrometry can be found in Supplemental Table S1 and Table S2, respectively, and in the Agilent application note 5994-0775en [37 ]. Two methods were used, a high-load and a low-load method, to determine the effect of high injection volumes/concentration on the number of annotations using the Agilent 6546 LC/Q-TOF.

Koelmel J.P., Li X., Stow S.M., Sartain M.J., Murali A., Kemperman R., Tsugawa H., Takahashi M., Vasiliou V., Bowden J.A., Yost R.A., Garrett T.J, & Kitagawa N. (2020). Lipid Annotator: Towards Accurate Annotation in Non-Targeted Liquid Chromatography High-Resolution Tandem Mass Spectrometry (LC-HRMS/MS) Lipidomics Using a Rapid and User-Friendly Software. Metabolites, 10(3), 101.

+ Open protocol

+ Expand

HPLC Analysis of Organic Acids in Bitter Gourd

Check if the same lab product or an alternative is used in the 5 most similar protocols

The concentration of organic acids was analyzed using an HPLC system (Thermo Vanquish) as per the method described previously [77 (link)] with modifications. The bitter gourd sample (0.1 g) was dissolved in distilled water (10 mL), sonicated (45 min), and centrifuged (5000 rpm, 10 min). A column (Agilent InfinityLab Poroshell 120 EC-C18; 4.6 × 50 mm, 2.7 μm) was used to separate the supernatant at 30 °C; the mobile phase was a 95:5 mixture of water and acetonitrile (both in 0.1% formic acid, HPLC grade). The retention time and calibration peaks were determined using standard solutions of oxalic acid, succinic acid, citric acid, and malic acid (Sigma-Aldrich, St. Louis, MO, USA) prepared in pure deionized water.

Zhang Y., Lu P., Jin H., Cui J., Miao C., He L., Yu J., Ding X, & Zhang H. (2023). Integrated Secondary Metabolomic and Antioxidant Ability Analysis Reveals the Accumulation Patterns of Metabolites in Momordica charantia L. of Different Cultivars. International Journal of Molecular Sciences, 24(19), 14495.

+ Open protocol

+ Expand

Peptide and Oligonucleotide Conjugate Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For peptides, LC-MS analyses were performed on an Agilent Technologies 1260 Infinity attached to a 6120 Quadrupole MS and a Peak Scientific NM32LA nitrogen generator. An Agilent InfinityLab Poroshell 120 EC-C18, 4.6 × 50 mm analytical LC column was used with a flow rate of 0.4 mL/min (solvent A: water + 0.1% TFA; solvent B: acetonitrile + 0.1% TFA). At the end of each run, columns were flushed with 100% solvent B for 4 min.
For PIP-CpG and NBP-CpG conjugates, MS analyses were performed on an Agilent 1290 Infinity LC attached to an Agilent 6530 Q-TOF mass spectrometer (no quadrupole filtering was used). LC method: an Agilent AdvancedBio Oligonucleotides (2.1 × 50 mm) analytical LC column was used with a flow rate of 0.5 mL/min at 50°C (solvent A: 15 mM triethylamine and 400 mM hexafluoroisopropanol in water; solvent B: methanol). Isocratic hold at 10% solvent B for 1 min, followed by linear gradient from 10% to 50% solvent B for 9 min during which time the conjugates eluted. Columns were flushed with 95% solvent B at the end of each run. MS method: Dual AJS ion source, negative polarity, 4 GHz resolution, 450–3200 m/z mass range, 3 spectra/sec MS scan rate. Reported deconvoluted mass spectra were obtained from 10 scans using Agilent’s MassHunter BioConfirm software.

Miller C.L., Sagiv-Barfi I., Neuhöfer P., Czerwinski D.K., Artandi S.E., Bertozzi C.R., Levy R, & Cochran J.R. (2021). Systemic delivery of a targeted synthetic immunostimulant transforms the immune landscape for effective tumor regression. Cell chemical biology, 29(3), 451-462.e8.

+ Open protocol

+ Expand

HPLC Analysis of Pharmaceutical Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC analysis was performed on a Waters HPLC system equipped with an
autosampler and binary pump (model e2695 separations module), dual absorbance
detector (model e2489 UV/Vis detector). Quantification of griseofulvin was
achieved using an Agilent InfinityLab Poroshell 120 EC-C18, 150 mm × 4.6
mm column maintained at 30°C. The mobile phase for griseofulvin comprised
of 40% acetonitrile, and 60% of 0.1% phosphoric acid solution in ultrapure
water. A flow rate of 1.0 mL/min and an injection volume of 5 μL were
used. The UV absorbance detector was set to 230 nm. Quantification of
ketoconazole was achieved using an Agilent Zorbax Eclipse XDB-C18, 150 mm
× 4.6 mm column maintained at 37°C. The mobile phase for
ketoconazole comprised of 47% acetonitrile, 52.9% of phosphoric acid solution
adjusted to pH 3.3, and 0.1% triethylamine. A flow rate of 1.2 mL/min and an
injection volume of 5 μL were used. The UV absorbance detector was set to
270 nm. Quantification of ibuprofen was achieved using an Agilent Zorbax SB
C-18, 150 mm × 4.6 mm column maintained at 30°C. The mobile phase
for ibuprofen comprised of 65% acetonitrile, 35% of 0.1% phosphoric acid in
ultrapure water. A flow rate of 1 mL/min and an injection volume of 5 μL
were used. The UV absorbance detector was set to 220 nm.

Jamil R, & Polli J.E. (2022). Prediction of In Vitro Drug Dissolution into Fed-state Biorelevant Media: Contributions of Solubility Enhancement and Relatively Low Colloid Diffusivity. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 173, 106179.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!