Three-hundred and ten non-duplicative diarrheal stool samples were collected from January to December 2015 from 0- to 12-year-old children (165 males and 145 females) admitted to Children’s Medical Center in Tehran, Iran. Suspected colonies were identified by routine microbiological and biochemical tests, including API-20E system kit (BioMerieux, France) and Shigella polyvalent agglutinating antisera (MAST, UK).
Api 20e system kit
Manufactured by bioMérieux
Sourced in France
The API-20E system kit is a biochemical identification test for the identification of Enterobacteriaceae and other non-fastidious Gram-negative rods. It consists of a plastic strip with 20 microtubes containing dehydrated biochemical test substrates. The kit allows for the identification of bacterial isolates through a series of standardized and miniaturized biochemical tests.
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Lab products found in correlation
2 protocols using api 20e system kit
1
Diarrheal Stool Samples from Iranian Children
2
Isolation and Identification of Enterobacteriaceae
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The organs were flamed using a Bunsen burner and cut into small pieces. Each piece was placed in a tube of pre-enrichment broth brain heart infusion broth (heart-brain broth) (Pasteur Institute of Algeria, IPA). After 24 h of incubation at 35°C, a drop from the broth was collected and inoculated on Hecktoen agar (IPA). The various inoculated plates were incubated at 35°C for 18 h. The appeared colonies were observed macroscopically; salmon yellow colonies were selected and seeded on nutrient agar (IPA) and incubated at 35°C for 18 h. The pure colonies were characterized microscopically by motility observation and by Gram-staining. Then, each colony was subsequently identified with an API 20 E system kit (Enterobacteriaceae) (Bio-Merieux, France).
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