The largest database of trusted experimental protocols

Mueller hinton broth and agar

Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom

Mueller Hinton (MH) broth and agar are culture media used for the cultivation and susceptibility testing of bacteria. MH broth provides nutrients to support the growth of a wide range of microorganisms, while MH agar is a solidified version of the broth used for the isolation and enumeration of bacteria.

Automatically generated - may contain errors

10 protocols using mueller hinton broth and agar

1

Antimicrobial and Antioxidant Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The medium Nutrient agar, Mueller–Hinton (agar and broth), and antibiotics discs were manufactured by Oxoid (Basingstoke, Hampshire, England). Folin-Ciocalteu reagent, quercetin, 2, 2-diphenyl-1picrylhydrazyl (DPPH), nitrite of sodium at 5%, and gallic acid were manufactured by Sigma Chemical (Saint-Louis, USA). All the other chemicals were analytical grade.
+ Open protocol
+ Expand
2

Reagent Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following medium: Nutrient agar, Mueller-Hinton (agar and broth) and antibiotics discs were from Oxoid (Basingstoke, Hampshire, England). Folin-Ciocalteu reagent, quercetin, 2, 2-diphenyl-1picrylhydrazyl (DPPH), 2, 4, 6- tripyridyl-s-trazine (TPTZ) and gallic acid were obtained from Sigma Chemical (Saint-Louis, USA). All the other chemicals were analytical grade.
+ Open protocol
+ Expand
3

Antimicrobial Susceptibility Testing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mueller Hinton agar and broth, Tryptone soya agar, Nutrient agar and broth and agar were obtained from Oxoid, Hampshire, England in dehydrated form.
Domperidone, atorvastatin, nifuroxazide, sulfamethoxazole-trimethoprim, ampicillin-sulbactam and mebeverine were obtained from Egyptian Pharmaceutical Industries Company (EPICO), Egypt, while vildagliptin and metformin were obtained from National Pharmaceutical Company (NAPCO), Egypt. Imipenem as Tienam® powder for IV infusion was the marketed pharmaceutical product manufactured by Merck & Co., Inc, USA. Linezolid, levofloxacin, amikacin and doxycycline were obtained from PHARCO Company, Egypt. Clindamycin was kindly granted from PFIZER Company. PCR master mix, Agarose DNA grade, DNA marker (100 bp), Tris-acetate-EDTA (TAE) buffer (50X), verapamil, vancomycin and the PCR primers13 (link) (table 1), provided by Integrated DNA Technologies were purchased from Sigma Aldrich, St. Louis, Mo, USA. DNase free water used for PCR reaction mixture was the product of Thermo Fisher Scientific, USA.
+ Open protocol
+ Expand
4

Microbial Culture Media Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
MacConkey agar, Mueller Hinton agar and broth, Triple sugar iron agar, Simmons citrate agar, Cystine lactose electrolyte deficient (CLED medium) and antibiotic discs were purchased from Oxoid, Hampshire, UK. Nutrient agar was obtained from Lab M limited, Lancoshine, UK. ESBL and AmpC D68C detection set discs were the products of MASTDISCS ID TM . Urea broth medium and Motility medium were prepared and sterilized according to Atlas (Atlas, 2004) . Other chemicals were of pharmaceutical grade.
+ Open protocol
+ Expand
5

Secnidazole Antimicrobial Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Secnidazole was purchased from Sigma-Aldrich (St. Louis, MO, USA). All the used solvents and chemicals were of pharmaceutical grade. All used media—Mueller–Hinton (MH) broth and agar, Luria–Bertani (LB) broth and agar, and Tryptone soy broth (TSB)—were purchased from Oxoid (Hampshire, UK).
+ Open protocol
+ Expand
6

CRISPR-Cas9 Editing of Salmonella Typhimurium

Check if the same lab product or an alternative is used in the 5 most similar protocols
S. enterica serovar Typhimurium NCTC 12023, and the S. Typhimurium ΔssaV mutant were kindly provided by Hensel’s lab (Germany). Plasmids pCRISPR and pCas9 were obtained from Addgene (http://www.addgene.org/, accessed on 12 May 2021) with No. 42875 and 42876, respectively [25 (link)]. Plasmids were introduced into S. enterica strains by electroporation, and recombinant strains were cultured in medium containing kanamycin (50 µg/mL), or chloramphenicol (25 µg/mL). All enzymes used to clone CRISPR plasmids and restriction endonuclease were provided from New England Biolabs, USA. Tryptone soy broth (TSB), Tryptic Soy Agar (TSA), Mueller Hinton (MH) broth and agar and Luria–Bertani (LB) broth and agar were purchased from Oxoid (Hampshire, UK). Dulbecco′s Modified Eagle′s Medium (DMEM) medium was obtained from Sigma-Aldrich (St. Louis, MO, USA). The used N-acylhomoserine lactones is N-hexanoyl-DL-homoserine lactone (CAS Number: 106983-28-2) was ordered from Sigma-Aldrich (St. Louis, MO, USA). All used chemicals were of pharmaceutical grade.
+ Open protocol
+ Expand
7

Bacterial Strains and Chemicals Used

Check if the same lab product or an alternative is used in the 5 most similar protocols
The used media, Luria–Bertani (LB) broth and agar, Mueller Hinton (MH) broth and agar, tryptone, and tryptone soya broth (TSB) were purchased from Oxoid (Hampshire, UK). All used chemicals were of pharmaceutical grade. The chemicals dimethyl sulphoxide (DMSO), glacial acetic acid, allopurinol, N-hexanoyl homoserine, elastin congo red, crystal violet, and lactone were purchased from Sigma–Aldrich (St. Louis, MO, USA). The bacterial P. aeruginosa PAO1 strain was obtained from the Department of Microbiology, Faculty of Pharmacy, Mansoura University, and the C. violaceum CV026 mutant strain was obtained from the Department of Microbiology, Faculty of Pharmacy, Ain Shams University.
+ Open protocol
+ Expand
8

Antimicrobial Assays for Beta-Blockers

Check if the same lab product or an alternative is used in the 5 most similar protocols
All microbiological media, Luria-Bertani (LB) broth and agar, Mueller Hinton (MH) broth and agar, and Tryptone soya broth (TSB) were obtained from Oxoid (Hampshire, UK). P. aeruginosa PAO1 (ATCC BAA-47-B1), C. violaceum CV026 (ATCC 31532) and S. enterica serovar typhimurium (NCTC 12023) were used in this study. β-blockers atenolol, esmolol, and metoprolol (CAS Numbers: 29122-68-7, 81161-17-3 and 56392-17-7, respectively) were ordered from Sigma-Aldrich (St. Louis, MO, USA). All the chemicals used were of pharmaceutical grade.
+ Open protocol
+ Expand
9

Antimicrobial Activity of Terazosin α-Blockers

Check if the same lab product or an alternative is used in the 5 most similar protocols
P. aeruginosa PAO1 (ATCC BAA-47-B1) and C. violaceum CV026 (ATCC 31532) were employed in this study. Terazosin α-blockers (CAS numbers: 63074-08-8) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Luria–Bertani (LB) broth and agar, tryptone soya broth (TSB), and Mueller–Hinton (MH) broth and agar were obtained from Oxoid (Hampshire, UK). The chemicals were of pharmaceutical grade.
+ Open protocol
+ Expand
10

Evaluating Anti-Virulence Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experiments were conducted using the model strain Pseudomonas aeruginosa PAO1 (ATCC BAA47B1) and Chromobacterium violaceum CV026 ATCC 31,532 that was used in the violacein inhibition assay. Tryptone soy broth (TSB), Mueller Hinton (MH) broth and agar and Luria–Bertani (LB) broth and agar were the products of Oxoid (Hampshire, UK). Vildagliptin (CAS 274901-16-5), sitagliptin (CAS 654671-77-9) and metformin (CAS 1115-70-4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Other used chemicals were of pharmaceutical grade. The dissolved drugs in dimethyl sulfoxide (DMSO) were usually prepared prior to each experiment and if needed they were kept at room temperature in dark place.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!