Immune cell isolation reagent kits

The RNAi kits of HAT1 and Fas, antibodies of Fas and HAT1 were purchased from Santa Cruz Biotech (Santa Cruz, CA). The recombinant TNF, FasL, PAR2-activating peptides SLIGRL and control peptide LRGILS were purchased from R&D Systems (Minneapolis, MN). The Annexin V kit and propidium iodide were purchased from Sigma Aldrich (St. Louis., MO). The reagents for performing RT-qPCR, Western blotting and gene transfection were purchased from Invitrogen (Carlsbad, CA). Immune cell isolation reagent kits were purchased from Miltenyi Biotech (San Diego, CA).

The EB1089 was purchased from Tocris Bioscience (Bristol, UK). The antibodies of VDR (D-6), CD3 (F7.2.38), CD28 (CD28.2), GATA3 (HG3-31), acH3 (AH3-120), acH4 (B-10), Foxp3 (2A11G9) and TGF-β (C-16) were purchased from Santa Cruz Biotech (Santa Cruz, CA). The fluorescence labeled antibodies for flow cytometry (flow cytometry) were purchased from BD Bioscience (Franklin Lakes, NJ). The ELISA kits of IL-4 and IFN-γ were purchased from R&D Systems (Minneapolis, MN). The immune cell isolation reagent kits were purchased from Miltenyi Biotech (San Diego, CA). The reagents and materials for RT-qPCR and Western blot were purchased from Invitrogen (Carlsbad, CA). The cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin, reagents for IP (immune precipitation) and ChIP (cromatin IP) were purchased from Sigma Aldrich (St. Louis., MO).