Male human Bronchial/Tracheal Epithelial Cells (HBTEC, Lifeline Cell Technology Cat# FC-0035; lot# 02196) were grown at 37°C in BronchiaLife Medium Complete Kit (Lifeline Cell Technology catalog number: LL-0023) in a 5% CO2 incubator until they reached confluence. Cells were then incubated 3 days more without addition of fresh media and were either mock infected or infected with the indicated Ad5-based vectors in the conditioned medium. All other cell lines were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum.
Bronchialife medium complete kit
Manufactured by Lifeline Cell Technology
The BronchiaLife medium complete kit is a laboratory product designed for culturing human bronchial epithelial cells. It provides a complete set of essential components, including basal medium, growth supplements, and other necessary additives, to support the growth and maintenance of these cell types in vitro.
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Lab products found in correlation
3 protocols using bronchialife medium complete kit
1
Culturing and Infecting Human Bronchial/Tracheal Cells
2
HBTEC Cell Culture and Infection
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Human bronchial/tracheal epithelial cells (HBTEC; catalog number FC-0035, lot number 02196; Lifeline Cell Technology) were grown at 37°C in a BronchiaLife medium complete kit (LL-0023; Lifeline Cell Technology) in a 5% CO2 incubator until they reached confluence. Cells were then incubated 3 days more without addition of fresh medium and were infected with the indicated Ad mutants under the conditioned medium.
3
HBTEC Infection and A-485 Treatment
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Human bronchial/tracheal epithelial cells (HBTEC; catalog number FC-0035, lot number 02196; Lifeline Cell Technology) were grown at 37°C in a BronchiaLife medium complete kit (LL-0023; Lifeline Cell Technology) in a 5% CO2 incubator until they reached confluence. Cells were then incubated 3 days more without addition of fresh medium and were infected for 12 hours with the indicated HAdV-5 mutants in the conditioned medium. A-485 (MedChemExpress) was added to a final concentration of 10 µM, or the same volume of DMSO vehicle was added, and cells were incubated for an additional 2 h.
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