Aldosterone

Manufactured by Thermo Fisher Scientific

Sourced in United States

Aldosterone is a laboratory equipment product used for the detection and measurement of aldosterone, a steroid hormone primarily produced by the adrenal glands. The core function of this product is to provide accurate and reliable quantitative analysis of aldosterone levels in biological samples.

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Lab products found in correlation

Cortisone, by Merck Group (3 mentions) Androsterone, by Merck Group (3 mentions) Epiandrosterone, by Merck Group (3 mentions) 11 deoxycortisol, by Merck Group (3 mentions) Testosterone, by Merck Group (3 mentions) Corticosterone, by Merck Group (3 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Dehydroepiandrosterone dhea, by Thermo Fisher Scientific (2 mentions) α estradiol, by Merck Group (2 mentions) β estradiol, by Merck Group (2 mentions) 8 isoprostane, by Cayman Chemical (1 mentions) Potassium acetate, by Merck Group (1 mentions) Lithium acetate, by Merck Group (1 mentions) Cesium acetate, by Merck Group (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Polyalanine, by Merck Group (1 mentions) Sodium acetate, by Merck Group (1 mentions) Corticosterone d4, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions)

4 protocols using aldosterone

Steroid Concentration Measurements

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Testosterone, androsterone, β-estradiol, α-estradiol, epiandrosterone, cortisone, corticosterone, 11-deoxycortisol, lithium acetate, sodium acetate, potassium acetate, and water were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aldosterone, dehydroepiandrosterone (DHEA), and methanol were purchased from Fisher Scientific (Pittsburg, PA, USA). Steroid solutions contained 25 μM of each steroid for the specified mixtures with 100–150 μM of lithium acetate, sodium acetate, or potassium acetate. The complex mixture contained 10 μM of all steroids and 100 μM of lithium acetate and 100 μM of potassium acetate.

Rister A.L., Martin T.L, & Dodds E.D. (2019). Formation of Multimeric Steroid Metal Adducts and Implications for Isomer Mixture Separation by Traveling Wave Ion Mobility Spectrometry. Journal of mass spectrometry : JMS, 54(5), 429-436.

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Quantification of Urinary Oxidative Stress Markers

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The concentration of nitrite/nitrate in urine was measured with a two-step Colorimetric Assay Kit (780001, Cayman, Ann Arbor, MI) for NO metabolite detection. The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step is the addition of the Griess reagent which converts nitrite into a deep purple azo compound and measured at 540 nm (Fisher, Pittsburgh, PA). Urinary nitrite/nitrate was corrected for creatinine concentration. Urine 8-isoprostane (516351, Cayman), 8-hydroxydeoxyguanosine (8-OHdG, 50154038, Fisher Scientific, Waltham, MA), aldosterone (50946889, Fisher), and serum renin (501044785, Fisher) were measured with ELISA kits.

Diao Z., Asico L.D., Villar V.A., Zheng X., Cuevas S., Armando I., Jose P.A, & Wang X. (2017). INCREASED RENAL OXIDATIVE STRESS IN SALT-SENSITIVE HUMAN GRK4γ486V TRANSGENIC MICE. Free radical biology & medicine, 106, 80-90.

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Steroid Analysis with Alkali Acetates

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Polyalanine, testosterone, androsterone, α-estradiol, β-estradiol, epiandrosterone, cortisone, corticosterone, 11-deoxycortisol, lithium acetate, sodium acetate, potassium acetate, rubidium acetate, cesium acetate, and water were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aldosterone, dehydroepiandrosterone (DHEA), methanol, and acetonitrile were purchased from Fisher Scientific (Pittsburg, PA, USA). Polyalanine was prepared at 12.5 μg/mL in 50% acetonitrile and 0.1% formic acid. Steroid solutions contained 50 μM of the given steroid and 100–150 μM of lithium acetate, sodium acetate, potassium acetate, rubidium acetate, or cesium acetate.

Rister A.L., Martin T.L, & Dodds E.D. (2018). Application of Group I Metal Adduction to the Separation of Steroids by Traveling Wave Ion Mobility Spectrometry. Journal of the American Society for Mass Spectrometry, 30(2), 248-255.

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Steroid Analysis by LC-MS/MS

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Testosterone, androsterone, epiandrosterone, cortisone, corticosterone, hydrocortisone, 11-deoxycortisol, corticosterone-d4, lithium acetate, sodium acetate, potassium acetate, and water were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aldosterone, dehydroepiandrosterone (DHEA), and methanol were purchased from Fisher Scientific (Pittsburg, PA, USA). The steroid mixture was made containing all steroids at a concentration of 500 nM in 50% HPLC grade water/methanol (the names, molecular weights, and structures of the steroids studied here are provided in Table S1 of the Supplementary Material). For analysis, 2.5 μL were injected into the LC (approximating 375 pg of each component on column). Water and methanol with lithium acetate added at a concentration of 150 μM (to form lithium ion adducts) were used as the mobile phase.

Rister A.L, & Dodds E.D. (2019). Liquid Chromatography-Ion Mobility Spectrometry-Mass Spectrometry Analysis of Multiple Classes of Steroid Hormone Isomers in a Mixture. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1137, 121941.

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