Immunohistochemistry was performed on 4-μm sections from formalin-fixed, paraffin-embedded (FFPE) tissue using a Ventana Benchmark XT automated stainer (Ventana Medical Systems, Tucson, AZ). The following antibodies were used: Melan-A (monoclonal mouse anti-human anti-body, A103; DAKO; 1:100), TFE3 (monoclonal mouse anti-human antibody, MRQ37, Cell Marque; prediluted), PAX8 (monoclonal mouse anti-human antibody, MRQ50, Cell Marque; prediluted), cytokeratin AE1/AE3 (monoclonal mouse anti-human antibody, AE1/AE3, Ventana; prediluted), cytokeratin 7 (monoclonal mouse anti-human antibody, OV-TL 12/30, Dako; 1:800), CAM 5.2 (monoclonal mouse anti-human antibody, BD, 1:100), Cathepsin K (monoclonal mouse anti-human antibody, 3F9, Cell marque, prediluted) and HBM45 (monoclonal mouse antihuman antibody, Dako; 1:60). The corresponding positive and negative controls were shown to be adequate.
Mrq37
Manufactured by Cell Marque
Sourced in United States
The MRQ37 is a piece of laboratory equipment designed for the analysis of biological samples. It is a compact and versatile instrument capable of performing various analytical tasks. The core function of the MRQ37 is to provide accurate and reliable data, enabling researchers and scientists to make informed decisions in their respective fields of study.
Automatically generated - may contain errors
4 protocols using mrq37
1
Immunohistochemistry Profiling of Tissue Samples
2
Immunocytochemical Staining of TFE3
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For immunocytochemical studies, 5 μm-thick sections were processed in an automated Ventana BenchMark IHC/ISH instrument using XT ultraview DAB v3 protocols (Ventana System, Tucson, AZ). A rabbit monoclonal anti-TFE3 antibody (MRQ-37, Cell Marque) was applied.
3
Immunohistochemical Analysis of TFE3 and Cathepsin K
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All 185 cases were analyzed for TFE3 and cathepsin K expression by immunohistochemistry. FFPE sections (4 μm in thickness) were cut and BOND-MAX autostainer and reagents (Leica BioSystems, Newcastle, UK) were used according to the manufacturer's instructions. Sections were labeled with primary antibodies for TFE3 (MRQ-37; Cell Marque, Rocklin, CA, USA; 1:100) and cathepsin K (3F9; Abcam, Cambridge, MA, USA; 1:1600). TFE3-positive cases were subdivided into moderate (2+) and strong (3+) expression groups based on labeling intensity, according to guidelines of a previous study [13 (link)]. Weak immunoreactivity (1+) was defined as subtle nuclear staining at low magnification. Weak (1+) or undetectable (0) TFE3 expression was considered as the TFE3-negative group [13 (link), 27 ].
4
Immunohistochemistry Profiling of Tissue Samples
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Immunohistochemistry was performed on 4-μm sections from formalin-fixed, paraffin-embedded (FFPE) tissue using a Ventana Benchmark XT automated stainer (Ventana Medical Systems, Tucson, AZ). The following antibodies were used: Melan-A (monoclonal mouse anti-human anti-body, A103; DAKO; 1:100), TFE3 (monoclonal mouse anti-human antibody, MRQ37, Cell Marque; prediluted), PAX8 (monoclonal mouse anti-human antibody, MRQ50, Cell Marque; prediluted), cytokeratin AE1/AE3 (monoclonal mouse anti-human antibody, AE1/AE3, Ventana; prediluted), cytokeratin 7 (monoclonal mouse anti-human antibody, OV-TL 12/30, Dako; 1:800), CAM 5.2 (monoclonal mouse anti-human antibody, BD, 1:100), Cathepsin K (monoclonal mouse anti-human antibody, 3F9, Cell marque, prediluted) and HBM45 (monoclonal mouse antihuman antibody, Dako; 1:60). The corresponding positive and negative controls were shown to be adequate.
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