Total RNA was extracted from VP-incubated cells utilizing the TRIzol regent (Invitrogen; Thermo Fisher Scientific, Inc.) using established protocol. cDNA was synthesized using the PrimeScript reverse transcription reagent kit (TIANGEN BIOTECH Co., Beijing, China) by following established guidelines. cDNA was assessed using qPCR on a SuperReal PreMix Plus (SYBR Green) system (TIANGEN BIOTECH Co.). The amplification conditions included: 95 °C for 15 min, 40 cycles of 95 °C for 20 s, 56 °C for 30 s and 68 °C for 30 s. The primer sequences used for qPCR were: YAP forward (F) 5′-TGACCCTCGTTTTGCCATGA-3′ and reverse (R), 5′-GTTGCTG CTGGTTGGAGTTG-3′; CTGF F, 5′-TGGAAGAGAACATTAAGAA GGGCA-3′ and R, 5′-TGCAGCCAGAAAGCTCAAAC-3′; AXL F, 5′-ACCCCAG AGGTGCTAATGGA-3′ and R, 5′-GTGGACTGGCTG TGCTTCC-3′; CYR61 F, 5′-GCAAGGAGCTGGGATTCGAT-3′ and R,5′-ATTCCAAAAACAGGGAGCCG-3′; GAPDH F, 5′-GCACCGTCAAGGCTGAGAAC-3′ and R, 5′-TGGTGAAGACGC CAGTGGA-3′. GAPDH functioned as the internal control. Gene expression was measured utilizing the 2-ΔΔCt method.
Superreal premix plus sybr green system
Manufactured by Tiangen Biotech
The SuperReal PreMix Plus (SYBR Green) system is a real-time PCR (polymerase chain reaction) reagent designed for quantitative gene expression analysis. It contains a premixed solution of SYBR Green I dye, Taq DNA polymerase, dNTPs, and buffer components necessary for real-time PCR reactions.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using superreal premix plus sybr green system
1
Quantifying Gene Expression in VP-treated Cells
2
Quantitative Analysis of Gene Expression
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Total RNA was obtained from VP-incubated cells utilizing the Trizol regent (Invitrogen; Thermo Fisher Scienti c, Inc.) as per established protocol. cDNA was made utilizing the PrimeScript reverse transcription reagent kit (TIANGEN BIOTECH Co., Beijing, China) as per established guidelines. cDNA was assessed through qPCR with a SuperReal PreMix Plus (SYBR Green) system (TIANGEN BIOTECH Co.). The ampli cation conditions include: 95˚C for 15 min, and then 40 cycles of 95˚C for 20 sec, 56˚C for 30 sec and 68˚C for 30 sec. The primer sequences used in qPCR were: YAP forward (F) 5'-TGACCCTCGTTTTGCCATGA-3' and reverse (R), 5'-GTT GCTGCTGGTTGGAGTTG-3'; CTGF F, 5'-TGGAAGAGAACATTAAGAAG GGCA-3' and R, 5'-TGCAGCCAGAAAGCTCAAAC-3'; AXL F, 5'-ACCCC AGAGGTGCTAATGGA-3' and R, 5'-GTGGACTGGCTG TGCTTCC-3'; CYR61 F, 5'-GCAAGGAGCTGGGATTCGAT-3' and R, 5'-ATTCCAAAAACAGGGAGCCG-3'; GAPDH F, 5'-GCACCGTCAAGG CTGAGAAC-3' and R, 5'-TGGTGAAGACGC CAGTGGA-3'. GAPDH functioned as internal control. Gene expression was measured utilizing the 2-ΔΔ Ct method.
3
Quantitative PCR Analysis of Cellular Genes
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Total RNA was obtained from VP-incubated cells utilizing the Trizol regent (Invitrogen; Thermo Fisher Scienti c, Inc.) as per established protocol. cDNA was made utilizing the PrimeScript reverse transcription reagent kit (TIANGEN BIOTECH Co., Beijing, China) as per established guidelines. cDNA was assessed through qPCR with a SuperReal PreMix Plus (SYBR Green) system (TIANGEN BIOTECH Co.). The ampli cation conditions include: 95˚C for 15 min, and then 40 cycles of 95˚C for 20 sec, 56˚C for 30 sec and 68˚C for 30 sec. The primer sequences used in qPCR were: YAP forward (F) 5'-TGACCCTCGTTTTGCCATGA-3' and reverse (R), 5'-GTT GCTGCTGGTTGGAGTTG-3'; CTGF F, 5'-TGGAAGAGAACATTAAGAAG GGCA-3' and R, 5'-TGCAGCCAGAAAGCTCAAAC-3'; AXL F, 5'-ACCCC AGAGGTGCTAATGGA-3' and R, 5'-GTGGACTGGCTG TGCTTCC-3'; CYR61 F, 5'-GCAAGGAGCTGGGATTCGAT-3' and R,5'-ATTCCAAAAACAGGGAGCCG-3'; GAPDH F, 5'-GCACCGTCAAGG CTGAGAAC-3' and R, 5'-TGGTGAAGACGC CAGTGGA-3'. GAPDH functioned as internal control. Gene expression was measured utilizing the 2-ΔΔ Ct method.
4
Quantitative Analysis of Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from VP-incubated cells utilizing the TRIzol regent (Invitrogen; Thermo Fisher Scienti c, Inc.) using established protocol. cDNA was synthesized using the PrimeScript reverse transcription reagent kit (TIANGEN BIOTECH Co., Beijing, China) by following established guidelines. cDNA was assessed using qPCR on a SuperReal PreMix Plus (SYBR Green) system (TIANGEN BIOTECH Co.). The ampli cation conditions included: 95˚C for 15 min, 40 cycles of 95˚C for 20 sec, 56˚C for 30 sec and 68˚C for 30 sec. The primer sequences used for qPCR were: YAP forward (F) 5'-TGACCCTCGTTTTGCCATGA-3' and reverse (R), 5'-GTTGCTGCTGGTTGGAGTTG-3'; CTGF F, 5'-TGGAAGAGAACATTAAGAA GGGCA-3' and R, 5'-TGCAGCCAGAAAGCTCAAAC-3'; AXL F, 5'-ACCCCAG AGGTGCTAATGGA-3' and R, 5'-GTGGACTGGCTG TGCTTCC-3'; CYR61 F, 5'-GCAAGGAGCTGGGATTCGAT-3' and R,5'-ATTCCAAAAACAGGGAGCCG-3'; GAPDH F, 5'-GCACCGTCAAGGCTGAGAAC-3' and R, 5'-TGGTGAAGACGC CAGTGGA-3'. GAPDH functioned as the internal control. Gene expression was measured utilizing the 2-ΔΔCt method.
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