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2 protocols using ninj2

1

Immunodetection of Neural Markers

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The antibodies used in immunofluorescence and western blot included Ninj2 (R&D systems, Cat# AF5056), Cleaved-Caspase 3 (Cell Signaling Technology, Cat# 9661), phosphorylated MLKL (abcam, Cat# ab196436), Laminin 211 (abcam, Cat# ab7463), Lama2 (Boster, Cat# BA3201-2), Sox10 (abcam, Cat# ab155279), Sox10 (abcam, Cat# ab216020), Mpz (abcam, Cat# ab31851), Neurofilament (abcam, Cat# ab8135), Ki67 (Gene Tex, Cat# GTX16667), phosphorylated FAK (abcam, Cat# ab4792), GAPDH (Proteintech, Cat# 60004-1-lg), HA (Santa Cruz, Cat# sc-7392), and FLAG (sigma, Cat# B3111).
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2

Immunofluorescence and Western Blot Antibodies

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The antibodies used in immunofluorescence and western blot included Ninj2 (Cat# AF5056, R&D systems), MBP (Cat# SMI‐94R, Covance), Olig2 (Cat# AB9610, Millipore), CC1 (Cat# OP80, Calbiochem), PDGFRα (Cat# SC‐338, Santa Cruz Biotechnology), Cleaved‐caspase 3 (Cat# 9661, Cell Signaling Technology), phosphorylated MLKL (Cat# ab196436, abcam), MLKL (Cat# 66675‐1‐lg, proteintech), TNFα (Cat# 3707, Cell Signaling Technology), BrdU (Cat# ab6326, abcam), Ki67 (Cat# GTX16667, Gene Tex), CNP (Cat# 5664, Cell Signaling Technology), GAPDH (Cat# 60004‐1‐lg, Proteintech), HA (Cat# SC‐7392, Santa Cruz Biotechnology), Flag (Cat# F‐1804, Sigma), and 488/555 donkey anti‐mouse/rabbit/rat secondary antibodies (Invitrogen).
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