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Conditioned freezing test chambers

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Conditioned freezing test chambers are designed to assess the freezing behavior of rodents in response to various stimuli. These chambers provide a controlled environment to study the animal's fear conditioning and memory processes.

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2 protocols using conditioned freezing test chambers

1

Contextual and Cued Fear Conditioning

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On the day following CWM, rats were placed in conditioned freezing test chambers (Coulbourn Instruments, Allentown, PA). On day-1, rats were placed in a test chamber for 10 min to habituate to the environment. Next, rats received 3 tone (82 dB, 2 kHz, 30 s duration) foot-shock pairings separated by 180 s. The tone (CS) foot-shock (US) pairings consisted of a 30 s tone with a 0.5 mA foot-shock during the last 1 s of the tone interval. Day-2 was the test of contextual memory. Rats were returned to the chamber for 6 min and freezing was measured with no tone or foot-shock. Day-3 was the test of cued memory. Rats were placed in the test chamber with a different, solid floor, for 6 min. During the first 3 min, no tone was presented. During the second 3 min the tone was presented without foot-shock. To assess extinction, after the 3 min tone, rats remained in the test chamber and received 30 alternating trials, each lasting 60 s with 30 s of tone followed by 30 s of no-tone. FreezeFrame software (Coulbourn Instruments) was used to determine the percentage of time spent freezing.
Sprowles J.L., Vorhees C.V, & Williams M.T. (2021). Impact of preweaning stress on long-term neurobehavioral outcomes in Sprague-Dawley rats: differential effects of barren cage rearing, pup isolation, and the combination. Neurotoxicology and teratology, 84, 106956.
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2

Conditioning and Contextual Fear Recall in Rats

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On P114, rats were placed in conditioned freezing test chambers (Coulbourn Instruments, Allentown, PA). Rats in each group were subdivided in two conditions each lasting 15 min: (a) tone pre-exposed (PE) and (b) not tone pre-exposed (NPE). PE rats were placed in a chamber and received 30 tones (82 dB, 2 kHz, 30 s duration) separated by 30 s intervals for 15 min. NPE rats received no tone during a comparable 15 min session. Following this rats were given 3 tone-footshock pairings (180 s between CS-US pairings) of 0.5 mA lasting 1 s. Contextual conditioning was assessed 24 h later. Rats were returned to the chamber for 6 min and freezing measured. Twenty-four hours later, rats were placed in the chambers with a different floor (grid rather than bars). Rats were assessed for 3 min with no-tone followed by 3 min with continuous tone. FreezeFrame software (Coulbourn Instruments) was used to determine the percentage of time spent freezing.
Sprowles J.L., Amos-Kroohs R., Braun A.A., Sugimoto C., Vorhees C.V, & Williams M.T. (2018). Developmental manganese, lead, and barren cage exposure have adverse long-term neurocognitive, behavioral and monoamine effects in Sprague-Dawley Rats. Neurotoxicology and teratology, 67, 50-64.
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